Hydrolysis of casein catalyzed by papain in n-propanol/NaCl two-phase system

Enzyme and Microbial Technology

Volumn 46, Issue 6, 2010, Pages 438-443

  Zhang, Yan ^a   Shi, Guqin ^a   Zhao, Fengsheng ^a  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

Author keywords

Amino acid; Casein; Enzymatic transformation; Hydrolysis; Papain; Two phase system

Indexed keywords

AROMATIC AMINO ACID; ENZYMATIC REACTION; ENZYMATIC TRANSFORMATION; FREE AMINO ACIDS; N-PROPANOL; PAPAIN; PARTITION COEFFICIENT; SINGLE PHASE; TWO PHASE SYSTEMS;

AMINO ACIDS; CASEIN; ORGANIC ACIDS; PHASE DIAGRAMS; PHOSPHATASES; PROPANOL; SODIUM CHLORIDE;

ENZYMATIC HYDROLYSIS;

CASEIN; HEXANE; MACROGOL; PAPAIN; PHENYLALANINE; PHOSPHATE; PROPANOL; SODIUM CHLORIDE; TRYPTOPHAN; TYROSINE; WATER;

AQUEOUS SOLUTION; ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SUBSTRATE; HYDROLYSIS; PH;

EID: 77950338653     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.01.003     Document Type: Article

References (19)

1. Heipieper H.J., Neumann G., Cornelissen S., Meinhardt F. Solvent-tolerant bacteria for biotransformations in two-phase fermentation systems. Appl Microbiol Biotechnol 2007, 74:961-973.
2. Wendhausena R., Frigato M., Fernandes P., Carvalho C.C.C.R., Cruza A., Pinheiro H.M., et al. Chrysotile as a support for the immobilisation of Mycobacterium sp. NRRL B-3805 cells for the bioconversion of γ-sitosterol in an organic-aqueous two-liquid phase system. J Mol Catal B: Enzym 2005, 32:61-65.
3. Lee I.Y., Volm T.G., Rosazza J.P.N. Decarboxylation of ferulic acid to 4-vinylguaiacol by Bacillus pumilus in aqueous-organic solvent two-phase systems. Enzyme Microb Technol 1998, 23:261-266.
4. Faizal I., Dozen K., Hong C.S., Kuroda A., Takiguchi N., Ohtake H., et al. Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system. J Ind Microbiol Biotechnol 2005, 32:542-547.
5. Parka O.J., Hollanda H.L., Khanb J.A., Vulfsonb E.N. Production of flavour ketones in aqueous-organic two-phase systems by using free and microencapsulated fungal spores as biocatalysts. Enzyme Microb Technol 2000, 26:235-242.
6. Cruz A., Fernandes P., Cabral J.M.S., Pinheiro H.M. Solvent partitioning and whole-cell sitosterol bioconversion activity in aqueous-organic two-phase systems. Enzyme Microb Technol 2004, 34:342-353.
7. Cruz A., Fernandes P., Cabral J.M.S., Pinheiro H.M. Whole-cell bioconversion of β-sitosterol in aqueous-organic two-phase systems. J Mol Catal B: Enzym 2001, 11:579-585.
8. Staebler A., Cruz A., van der Goot W., Pinheiro H.M., Cabral J.M.S., Fernandes P. Optimization of androstenedione production in an organic-aqueous two-liquid phase system. J Mol Catal B: Enzym 2004, 29:19-23.
9. Bie S., Du L., Zhang L., Lu F. Bioconversion of methyl-testosterone in a biphasic system. Proc Biochem 2005, 40:3309-3313.
10. Sandford V., Breuer M., Hauer B., Rogers P., Rosche B. (R)-phenylacetylcarbinol production in aqueous/organic two-phase systems using partially purified pyruvate decarboxylase from Candida utilis. Biotechnol Bioeng 2005, 91:190-198.
11. Rosche B., Breuer M., Hauer B., Rogers P.L. Biphasic aqueous/organic biotransformation of acetaldehyde and benzaldehyde by Zymomonas mobilis pyruvate decarboxylase. Biotechnol Bioeng 2004, 86:788-794.
12. Fujiwara S., Yamanaka A., Yamada Y., Hirooka K., Higashibata H., Fukuda W., et al. Efficient synthesis of trans-polyisoprene compounds using two thermostable enzymes in an organic-aqueous dual-liquid phase system. Biochem Biophys Res Commun 2008, 365:118-123.
13. Teotia S., Sharma S., Gupta M.N. Casein hydrolysis in PEG-salt two-phase system by eudragit-chymotrypsin bioconjugate. Biotechnol Lett 2003, 25:637-639.
14. Chilow G.G., Svedas V.K. Enzymatic hydrolysis of β-lactam antibiotics at low pH in a two-phase " aqueous solution-water-immiscible organic solvent" system. Can J Chem 2002, 80:699-707.
15. Sharma S., Teotia S., Gupta M.N. Bioconversion in an aqueous two-phase system using a smart biocatalyst: casein hydrolysis by a α-chymotrypsin derivative. Enzyme Microb Technol 2003, 32:337-339.
16. Azarkan M., Moussaoui A.E., van Wuytswinkel D., Dehon G., Looze Y. Fractionation and purification of the enzymes stored in the latex of Carica papaya. J Chromatogr B 2003, 790:229-238.
17. Baines B.S., Brocklehurst K. Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active center properties that differ from those of papain by using 2,2′-dipyridyl disulfide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Biochem J 1982, 205:205-211.
18. Barrett A.J., Rawlings N.D., Woessner J.F. Introduction: cysteine peptidases and their clans. Handbook of proteolytic enzymes 1998, Academic Press, San Diego, p. 545-66.
19. Morrice N.A., Carrey E.A. Peptide mapping. Protein structure: a practical approach 1997, 123. Oxford University Press, New York. 2nd ed. T.E. Creighton (Ed.).