Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy

Biochemical Journal

Volumn 385, Issue 2, 2005, Pages 511-517

  Natalello, Antonino ^a,b   Ami, Diletta ^a,b   Brocca, Stefania ^a   Lotti, Marina ^a   Doglia, Silvia M ^a,b  

^a UNIVERSITY OF MILANO BICOCCA   (Italy)

^b Istituto Nazionale per la Fisica della Materia Milan   (Italy)

Author keywords

Candida rugosa lipase; Conformational stability; Heterologous protein glycosylation; Infrared spectroscopy; MS; Secondary structure

Indexed keywords

CONFORMATIONS; DATA ACQUISITION; FERMENTATION; FOURIER TRANSFORM INFRARED SPECTROSCOPY; THERMODYNAMIC STABILITY; X RAYS;

GLYCOSYLATION; LIPASE 1; PROTEIN SUGAR MOIETIES; PROTEIN UNFOLDING;

ENZYME KINETICS;

CARBOHYDRATE; MANNOSE; TRIACYLGLYCEROL LIPASE; WATER;

ABSORPTION; ALPHA HELIX; ARTICLE; CANDIDA RUGOSA; DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME STRUCTURE; FERMENTATION; HIGH TEMPERATURE PROCEDURES; INFRARED SPECTROSCOPY; MASS SPECTROMETRY; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; STRUCTURE ANALYSIS; X RAY ANALYSIS;

AMIDES; CANDIDA; CARBOXYLIC ESTER HYDROLASES; FUNGAL PROTEINS; GLYCOSYLATION; HEAT; LIPASE; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; SOLUTIONS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

CANDIDA RUGOSA; PICHIA PASTORIS;

EID: 12844274977     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041296     Document Type: Article

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