Cloning, expression, purification and characterization of a single chain variable fragment specific to tumor necrosis factor alpha in Escherichia coli

Journal of Biotechnology

Volumn 156, Issue 4, 2011, Pages 238-244

  Sushma, Krishnan ^a   Vijayalakshmi, Mookambeswaran A ^a,b   Krishnan, Venkataraman ^a   Satheeshkumar, Padikara Kutty ^a  

^a VIT UNIVERSITY   (India)

^b UNIVERSITÉ DE TECHNOLOGIE DE COMPIÈGNE   (France)

Author keywords

Cytotoxicity; Immobilized metal affinity chromatography (IMAC); Single chain variable fragment (ScFv); Soluble protein; Tumor necrosis factor (TNF )

Indexed keywords

AFFINITY CHROMATOGRAPHY; CYTOTOXICITY; DISEASES; ESCHERICHIA COLI; GLYCOPROTEINS; MACROPHAGES; METAL IONS; MOLECULES; MONOCLONAL ANTIBODIES; PURIFICATION; SUPPORT VECTOR MACHINES; TUMORS;

ANION EXCHANGE CHROMATOGRAPHY; CONVECTIVE INTERACTION MEDIA (CIM); IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; METHACRYLATE MONOLITHS; SINGLE CHAIN VARIABLE FRAGMENTS; SOLUBLE PROTEINS; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTORS;

CHAINS;

COPPER ION; IMMUNOGLOBULIN G ANTIBODY; METHACRYLIC ACID; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR ALPHA ANTIBODY;

ANION EXCHANGE CHROMATOGRAPHY; ANTIBODY PRODUCTION; ARTICLE; CELL STRAIN MCF 7; CONTROLLED STUDY; CYTOTOXICITY; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IC 50; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ESCHERICHIA COLI;

EID: 82955187801     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.06.039     Document Type: Article

References (58)

1. Andersen D.C., Reilly D.E. Production technologies for monoclonal antibodies and their fragments. Curr. Opin. Biotechnol. 2004, 15:456-462.
2. Arbabi-Ghahroudi M., Tanha J., MacKenzie R. Prokaryotic expression of antibodies. Cancer Metastasis Rev. 2005, 24:501-519.
3. Bird R.E., Hardman K.D., Jacobsen J.W., Johnson S., Kaufman B.M., Lee S.M. Single-chain antigen-binding proteins. Science 1988, 242:426-432.
4. Bradford M.M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
5. Brne P., Podgornik A., Benčina K., Gabor B., Štrancar A., Peterka M. Fast and efficient separation of immunoglobulin M from immunoglobulin G using short monolithic columns. J. Chromatogr. A 2007, 1144(1):120-125.
6. Caron de Fromentel C., Gruel N., Venot C., Debussche L., Conseiller E., Dureuil C., Teillaud J.L., Tocque B., Bracco L. Restoration of transcriptional activity of p53 mutants in human tumour cells by intracellular expression of anti p53 single chain Fv fragments. Oncogene 1999, 18:551-557.
7. Casey J.L., Keep P.A., Chester K.A., Robson L., Hawkins R.E., Begen R.H.J. Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography. J. Immunol. Methods 1995, 179:105-116.
8. Cazzola M., Polosa R. Anti TNF-α and Th1 cytokine directed therapies for the treatment of asthma. Curr. Opin. Allergy Clin. Immunol. 2006, 6:43-50.
9. Chang H., Qin W., Li Y., Zhang J., Lin Z., Lv M., Sun Y., Feng J., Shen B. A novel human ScFv fragment against TNF-α from denovo design method. Mol. Immunol. 2007, 44:3789-3796.
10. Choi G.H., Lee D.H., Min W.K., Cho Y.J., Kweon D.H., Son D.H., Park K., Seo J.H. Cloning, expression, and characterization of single-chain variable fragment antibody against mycotoxin deoxynivalenol in recombinant Escherichia coli. Protein Expr. Purif. 2005, 35:84-92.
11. Denzin L.K., Voss E.W. Construction, characterization, and mutagenesis of an anti fluorescein single chain antibody idiotype family. J. Biol. Chem. 1992, 267:8925-8931.
12. Desplancq D., King D.J., Lawson A.D.G., Mountain A. Multimerization behavior of single chain Fv variants for the tumour-binding antibody B72.3. Protein Eng. 1994, 7:1027-1033.
13. Diaz-Ricci J.C., Hernández M.E. Plasmid effects on Escherichia coli metabolism. Crit. Rev. Biotechnol. 2000, 20:79-108.
14. Dübel S., Breitling F., Klewinghaus I., Little M. Regulated secretion and purification of recombinant antibodies in E. coli. Cell Biophys. 1992, 21:69-79.
15. Etzel M.R., Bund T. Monoliths for the purification of whey protein-dextran conjugates. J. Chromatogr. A 2011, 1218:2445-2450.
16. Forstberg G., Forsgren M., Jaki M., Norin M., Sterky C., Enhörning A., Larsson K., Ericsson M., Björk P. Identification of framework residues in a secreted recombinant antibody fragment that control production level and localization in E. coli. J. Biol. Chem. 1997, 272:12430-12436.
17. Geng S., Chang H., Qin W., Li Y., Feng J., Shen B. Overexpression, effective renaturation, and bioactivity of novel single-chain antibodies against TNF-α. Prep. Biochem. Biotechnol. 2008, 38:74-86.
18. Grim J., Deshane J., Feng M., Lieber A., Kay M., Curiel D.T. erbB-2 knockout employing an intracellular single-chain antibody (ScFv) accomplishes specific toxicity in erbB-2-expressing lung cancer cells. Am. J. Respir. Cell Mol. Biol. 1996, 15:348-354.
19. Hoedemaeker F.J., Signorelli T., Johns K., Kuntz D.A., Rose D.R. A single chain Fv fragment of P-glycoprotein-specific monoclonal antibody C219. Design, expression and crystal structure at 2.4Å resolution. J. Biol. Chem. 1997, 272:29784-29789.
20. Hoffmann F., Rinas U. Stress induced by recombinant protein production in Escherichia coli. Adv. Biochem. Eng. Biotechnol. 2004, 89:73-92.
21. Huston J.S., Levinson D., Mudgett-Hunter M., Tai M.S., Novotny J., Margolies M.N. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. 1988, 85:5879-5883.
22. Isobe K., Kawakami Y. Application of two types of CIM tube column for purification of microbial enzymes. J. Chromatogr. A 2005, 1065:129-134.
23. Joosten V., Lokman C., Van Den Hondel C.A., Punt P.J. The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. Microb. Cell Fact. 2003, 2:1-3.
24. Josic D., Buchacher A., Jungbauer A. Monoliths as stationary phases for separation of proteins and polynucleotides and enzymatic conversion. J. Chromatogr. B 2001, 752(2):191-205.
25. Kavanaugh A., McInnes I., Mease P., Krueger G.G., Gladman D., Gomez-Reino J., Papp K., Zrubek J., Mudivarthy S., Mack M., Visvanathan S., Beutler A. Golimumab, a new human tumor necrosis factor alpha antibody, administered every four weeks as a subcutaneous injection in psoriatic arthritis: twenty-four-week efficacy and safety results of a randomized, placebo-controlled study. Arthritis Rheum. 2009, 60:976-986.
26. Keane J., Gershon S., Wise R.P., Mirabile-Levens E., Kasznica L., Schwieterman W.D., Siegel J.N., Braun M.M. Tuberculosis associated with Infliximab, a tumor necrosis factor-alpha neutralizing agent. N. Engl. J. Med. 2001, 345:1098-1104.
27. Kollias G., Douni E., Kassiotis G., Kontoyiannis D. On the role of tumor necrosis factor and receptors in models of multi organ failure, rheumatoid arthritis, multiple sclerosis and inflammatory bowel disease. Immunol. Rev. 1999, 169:175-194.
28. Laemmli U.K. Cleavage of a structural protein during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
29. Lim K.P., Li H., Nathan S. Expression and purification of a recombinant ScFv towards the exotoxin of the pathogen, Burkholderia pseudomallei. J. Microbiol. 2004, 42:126-132.
30. Lipsky P.E., van der Heijde D.M., St Clair E.W., Furst D.E., Breedveld F.C., Kalden J.R., Smolen J.S., Weisman M., Emery P., Feldmann M. Infliximab and methotrexate in the treatment of rheumatoid arthritis, anti-tumor necrosis factor trial in rheumatoid arthritis with concomitant therapy study group. N. Engl. J. Med. 2000, 343:1594-1602.
31. Liu M., Wang X., Yin C., Zhang Z., Lin Q., Zhen Y., Huang H. A novel bivalent single-chain variable fragment (ScFv) inhibits the action of tumour necrosis factor alpha. Biotechnol. Appl. Biochem. 2008, 50:173-179.
32. Lu M., Gong X.G., Yu H., Li J.Y. Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag. J. Zhejiang Univ. Sci. 2005, 6B8:832-837.
33. Mallender W.D., Carrero J., Voss E.W. Comparative properties of the single chain antibody and Fv derivatives of mAb 4-4-20. J. Biol. Chem. 1996, 271:5338-5346.
34. Maini R., Clair E.W.St., Breedveld F., Frust D., Kalden J., Weisman M., Smolen J., Emery P., Harriman G., Feldmann M. Infliximab (chimeric anti-tumor necrosis factor α monoclonal antibody) versus placebo in rheumatoid arthritis patients receiving concomitant methotrexate: a randomized phase III trial. Lancet 1999, 354:1932-1939.
35. Maini R.N., Brennan F.M., Williams R. TNF-alpha in rheumatoid arthritis and prospects of anti TNF therapy. Clin. Exp. Rheumatol. 1993, 11:173-175.
36. Merk H., Stiege W., Tsumoto K., Kumagai I., Erdmann V.A. Cell-free expression of two single-chain monoclonal antibodies against lysozyme: effect of domain arrangement on the expression. J. Biochem. 1999, 125:328-333.
37. Miller K.D., Weaver-Feldhaus J., Gray S.A., Siegel R.W., Feldhaus M.J. Production, purification, and characterization of human ScFv antibodies expressed in Saccharomyces cerevisiae, Pichia pastoris, and Escherichia coli. Protein Expr. Purif. 2005, 42:255-267.
38. Mönstera A., Hiller O., Grügerb D., Blasczykb R., Kaspera C. Isolation and purification of blood group antigens using immuno-affinity chromatography on short monolithic columns. J. Chromatogr. A 2011, 1218:706-710.
39. Mukai Y., Okamoto T., Kawamura M., Shibata H., Sugita T., Imai S., Abe Y., Nagano K., Nomura T., Kamada, Tsutsumi Y., Mayumi T., Nakagawa S., Tsunoda S. Optimization of anti-tumor necrosis factor-alpha single chain Fv displayed on phages for creation of functional antibodies. Pharmazie 2006, 61:889-890.
40. Palladino M.A., Bahjat F.R., Theodorakis E.A., Moldawer L.L. Anti-TNF-α therapies: the next generation. Nat. Rev. Drug Discov. 2003, 2:736-746.
41. Peterka M., Jarc M., Banjac M., Francovic V., Bendcina K., Merhar M., Menart V., Strancar A., Podgornik A. Characterization of metal chelate methacrylate monoliths. J. Chromatogr. A 2006, 1109(1):80-85.
42. Porath J., Olin B. Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials: serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 1983, 22:1621-1630.
43. Power B.E., Hudson P.J. Synthesis of high avidity antibody fragments for cancer imaging. J. Immunol. Methods 2000, 242:193-204.
44. Prasanna R.R., Vijayalakshmi M.A. Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G. J. Chromatogr. A 2010, 1217(23):3660-3667.
45. Price M.R., Sekowski M., Tendler S.J.B. Purification of anti-epithelical mucin monoclonal antibodies by epitope affinity chromatography. J. Immunol. Methods 1991, 139:83-90.
46. Richardson J.H., Marasco W.A. Intracellular antibodies: development and therapeutic potential. Trends Biotechnol. 1995, 13:306-310.
47. Rippmann J.F., Klein M., Hoischen C., Brocks B., Rettig W.J., Gumpert J., Pfizenmaier K., Mattes R., Moosmayer D. Prokaryotic expression of ScFv antibodies: secretion in L-form cells of Proteus mirabilis leads to active and overcomes the limitations of periplasmic expression in E. coli. Appl. Environ. Microbiol. 1998, 64:4862-4869.
48. Smallshaw J.E., Georges F., Lee J.S., Waygood E.B. Synthesis, cloning & expression of single-chain Fv gene of the Hpr specific monoclonal antibody, Jel 42. Determination of binding constants with wild-type and mutant HPrs. Protein Eng. 1999, 12:623-630.
49. Sulkowski E. The saga of IMAC and MIT. Bioessays 1989, 10:170-175.
50. Svec F., Tennikova T.B., Deyl Z. Historical review. Monolithic Materials: Preparation, Properties, and Applications 2003, Elsevier, Amsterdam, pp. 1-18.
51. Tennikova T.B., Freitag R. An introduction to monolithic disks as stationary phases for high performance biochromatography. J. High Resolut. Chromatogr. 2000, 23:27-38.
52. Tennikova T.B., Svec F. High-performance membrane chromatography: highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes. J. Chromatogr. 1993, 646:279-288.
53. Todorova-Balvay D., Pitiot O., Bourhim M., Srikrishnan T., Vijayalakshmi M. Immobilized metal-ion affinity chromatography of human antibodies and their proteolytic fragments. J. Chromatogr. B 2004, 808(1):57-62.
54. Tong Q., Liu K., Lu X.M., Shu X.G., Wang G.B. Construction and characterization of a novel fusion protein MG7-ScFv/SEB against gastric cancer. J. Biomed. Biotechnol. 2010, 121094-121102.
55. Vancan S., Miranda E.A., Bueno S.M.A. IMAC of human IgG: studies with IDA-immobilized copper, nickel, zinc, and cobalt ions and different buffer systems. Process Biochem. 2002, 37(6):573-579.
56. Wang H., Dai J., Li B., Fan K., Peng L., Zhang D. Expression, purification, and characterization of an immunotoxin containing a humanized anti-CD25 single chain fragment variable antibody fused to a modified truncated Pseudomonas exotoxin A. Protein Expr. Purif. 2008, 58:140-147.
57. Yang J., Chen R., Wei J., Zhang F., Zhang Y., Jia L., Yan Y., Luo W., Cao Y., Yao L., Sun J., Xu Z., Yang A. Production and characterization of a recombinant single-chain antibody against Hantaan virus envelop glycoprotein. Appl. Microbiol. Biotechnol. 2010, 86:1067-1075.
58. Yokota T., Milenic D., Whitlow M., Schlom J. Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms. Cancer Res. 1992, 52:19923402-19923408.