Fast and efficient separation of immunoglobulin M from immunoglobulin G using short monolithic columns

Journal of Chromatography A

Volumn 1144, Issue 1, 2007, Pages 120-125

  Brne, P ^a   Podgornik, A ^a   Bencina K ^a   Gabor, B ^a   Strancar A ^a   Peterka, M ^a  

^a BIA SEPARATIONS D O O   (Slovenia)

Author keywords

Chromatography; CIM monoliths; IgG; IgM

Indexed keywords

CHROMATOGRAPHY; IMMUNOLOGY; ION EXCHANGE; PURIFICATION; SEPARATION;

CIM MONOLITHS; CONVECTIVE INTERACTION MEDIA (CIM); IGG; IGM;

PROTEINS;

IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; METHACRYLIC ACID; SERUM ALBUMIN;

ANION EXCHANGE; ARTICLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; FLOW RATE; HUMAN; HUMAN CELL; ION EXCHANGE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PURIFICATION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; REPRODUCIBILITY OF RESULTS; SERUM ALBUMIN;

EID: 33847039612     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2006.12.044     Document Type: Article

References (17)

1. Nezlin R. In: Van Oss C.J., and Van Regenmortel Marck H.V. (Eds). Immunochemistry (1994), Marcel Dekker, New York 3
2. Josic D., and Buchacher A. J. Biochem. Biophys. Methods 49 (2001) 153
3. Coffinier Y., Legallais C., and Vijayalakshmi M.A. J. Membr. Sci. 208 (2002) 13
4. Verdoliva A., Pannone F., Rossi M., Catello S., and Manfredi V. J. Immunol. Methods 271 (2002) 77
5. Lowe C.R., Lowe A.R., and Gupta G. J. Biochem. Biophys. Methods 49 (2001) 561
6. Tornoe I., Titlestad I.L., Kejling K., Erb K., Ditzel H.J., and Jensenius J.C. J. Immun. Methods 205 (1997) 11
7. Nevens J., Mallia A.K., Wendt M.W., and Smith P. J. Chromatogr. 597 (1992) 247
8. One Step Purifications of IgM and IgY with HiTrap Purification Columns, Life Science News 3, Amersham, Uppsala, 1999.
9. Johnson R.B., and Libby R. J. Clin. Microbiol. 12 (1980) 451
10. Babesia microti IgM IFA Antibody Kit; Fuller Laboratories, Fullerton, CA, 1999.
11. Graham D.A., Mawhinney K.A., German A., Foster J.C., Adair B.M., and Merza M. J. Vet. Diagn. Invest. 11 (1999) 127
12. Caul E.O., Hobbs S.J., Roberts P.C., and Clarke S.K.R. J. Med. Virol. 2 (1978) 153
13. Skaug K., and Gaarder P.I. Acta Pathol. Microbiol. Scand. Sect. C 86 (1978) 33
14. Sugg U., Schneider W., Frosner G.G., and Schmidt K. Vox. Sang. 36 (1979) 25
15. Krech U., and Wilhem J.A. J. Gen. Virol. 44 (1979) 281
16. Štrancar A., Podgornik A., Barut M., and Necina R. In: Freitag R. (Ed). Advances in Biochemical Engineering/Biotechnology (Modern Advances in Chromatography vol. 76 (2002), Springer, Heidelberg 49
17. Štrancar A., Barut M., Podgornik A., Koselj P., Josić D., and Buchacher A. LC-GC Int. 11 (1998) 660