Procaryotic expression of single-chain variable-fragment (scFv) antibodies: Secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coli

Applied and Environmental Microbiology

Volumn 64, Issue 12, 1998, Pages 4862-4869

  Rippmann, Jörg F ^a   Klein, Michaela ^a   Hoischen, Christian ^b   Brocks, Bodo ^a   Rettig, Wolfgang J ^c   Gumpert, Johannes ^b   Pfizenmaier, Klaus ^a   Mattes, Ralf ^a   Moosmayer, Dieter ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b FRITZ LIPMANN INSTITUTE   (Germany)

^c BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; IMMUNOGLOBULIN FRAGMENT;

ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BACTERIAL GROWTH; CONCENTRATION (PARAMETERS); CYTOPLASM; ESCHERICHIA COLI; GROWTH INHIBITION; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR CLONING; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEUS MIRABILIS; PROTOPLAST; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA; MIRABILIS; NEGIBACTERIA; PROKARYOTA; PROTEUS MIRABILIS;

EID: 0031766998     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.12.4862-4869.1998     Document Type: Article

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