Synthesis, cloning and expression of the single-chain Fv gene of the HPr-specific monoclonal antibody, Jel42. Determination of binding constants with wild-type and mutant HPrs

Protein Engineering

Volumn 12, Issue 7, 1999, Pages 623-630

  Smallshaw, Joan E ^a,c   Georges, Fawzy ^b   Lee, Jeremy S ^a   Waygood, E Bruce ^a  

^a UNIVERSITY OF SASKATCHEWAN   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^c UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

Antibody; Gene synthesis; HPr; Protein binding constant; Protein folding; Single chain Fv

Indexed keywords

CARRIER PROTEIN; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; PHOSPHOENOLPYRUVATE SUGAR PHOSPHOTRANSFERASE; RESTRICTION ENDONUCLEASE; RNA POLYMERASE II;

ANTIBODY SPECIFICITY; ANTIBODY STRUCTURE; ANTIGEN BINDING; ARTICLE; CARBOXY TERMINAL SEQUENCE; CODON USAGE; DISSOCIATION CONSTANT; DNA SEQUENCE; IMMUNOGLOBULIN GENE; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN FOLDING;

EID: 0032803526     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/12.7.623     Document Type: Article

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