Characterization of metal chelate methacrylate monolithic disk for purification of polyclonal and monoclonal immunoglobulin G

Journal of Chromatography A

Volumn 1217, Issue 23, 2010, Pages 3660-3667

  Prasanna, Rajasekar R ^a   Vijayalakshmi, Mookambeswaran A ^a,b  

^a VIT UNIVERSITY   (India)

^b UNIVERSITÉ DE TECHNOLOGIE DE COMPIÈGNE   (France)

Author keywords

Buffers; Convective interaction media (CIM) monolithic disk; Dynamic binding capacity; IgG purification; Immobilized metal affinity chromatography (IMAC)

Indexed keywords

AS-LIGANDS; BINDING CAPACITIES; BINDING PROPERTIES; BIOLOGICAL SAMPLES; BUFFER SYSTEM; CONVECTIVE INTERACTION MEDIA; DYNAMIC BINDING CAPACITY; HIGH-FLOW RATE; HUMAN IGG; HUMAN IMMUNOGLOBULIN; HUMAN SERUM; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOGLOBULIN G; LARGE BIOMOLECULES; LOW BACK; METAL CHELATE; METHACRYLATE MONOLITHS; MONOLITHIC DISKS; POLYCLONAL; STATIONARY PHASE;

ADSORPTION; AFFINITY CHROMATOGRAPHY; BINDING ENERGY; CHELATION; DESORPTION; DISKS (STRUCTURAL COMPONENTS); METAL RECOVERY; METALS; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; PURIFICATION; ZINC;

METAL REFINING;

COPPER; IMMUNOGLOBULIN G; METAL CHELATE; METHACRYLIC ACID DERIVATIVE; NICKEL; PARAPROTEIN; ZINC;

ADSORPTION; ARTICLE; BINDING AFFINITY; CHEMICAL ANALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; DESORPTION; HUMAN; LIGAND BINDING; PH; PRIORITY JOURNAL; PROTEIN BINDING; PURIFICATION;

ADSORPTION; ANIMALS; ANTIBODIES, MONOCLONAL; ASCITES; BUFFERS; CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; METALS, HEAVY; METHACRYLATES; MICE; PROTEIN BINDING;

EID: 77952668608     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.03.058     Document Type: Article

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