Inducible gene manipulations in brain serotonergic neurons of transgenic rats

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  Weber, Tillmann ^a   Schönig, Kai ^a   Tews, Björn ^b   Bartsch, Dusan ^a,c  

^a CENTRAL INSTITUTE OF MENTAL HEALTH   (Germany)

^b University of Zurich Brain Research Institute   (Switzerland)

^c UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CRE RECOMBINASE; ENHANCED GREEN FLUORESCENT PROTEIN; SEROTONIN; TAMOXIFEN; TRYPTOPHAN HYDROXYLASE 2;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; AUTONOMIC NERVOUS SYSTEM FUNCTION; CONTROLLED STUDY; GENE CASSETTE; GENE DELETION; GENE DOSAGE; GENE OVEREXPRESSION; GENETIC ENGINEERING; GENETIC RECOMBINATION; INDUCIBLE GENE MANIPULATION; LOXP SITE; NONHUMAN; PROTEIN EXPRESSION; RAT; REGULATORY SEQUENCE; SEROTONINERGIC NERVE CELL; SEROTONINERGIC SYSTEM; TISSUE SPECIFICITY; TRANSGENIC RAT;

ANIMALS; BETA-GALACTOSIDASE; BRAIN; GENE DOSAGE; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; INTEGRASES; MICE; RAPHE NUCLEI; RATS; RATS, TRANSGENIC; RECOMBINATION, GENETIC; SEROTONERGIC NEURONS; TRYPTOPHAN HYDROXYLASE;

EID: 82255162415     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0028283     Document Type: Article

References (46)

1. Cools R, Roberts AC, Robbins TW, (2008) Serotoninergic regulation of emotional and behavioural control processes. Trends Cogn Sci 12: 31-40.
2. Murphy DL, Fox MA, Timpano KR, Moya PR, Ren-Patterson R, et al. (2008) How the serotonin story is being rewritten by new gene-based discoveries principally related to SLC6A4, the serotonin transporter gene, which functions to influence all cellular serotonin systems. Neuropharmacology 55: 932-960.
3. Leonardo ED, Hen R, (2008) Anxiety as a developmental disorder. Neuropsychopharmacology 33: 134-140.
4. Alenina N, Kikic D, Todiras M, Mosienko V, Qadri F, et al. (2009) Growth retardation and altered autonomic control in mice lacking brain serotonin. Proc Natl Acad Sci U S A 106: 10332-10337.
5. Liu C, Maejima T, Wyler SC, Casadesus G, Herlitze S, et al. (2010) Pet-1 is required across different stages of life to regulate serotonergic function. Nat Neurosci 13: 1190-1198.
6. Zhao ZQ, Gao YJ, Sun YG, Zhao CS, Gereau RWt, et al. (2007b) Central serotonergic neurons are differentially required for opioid analgesia but not for morphine tolerance or morphine reward. Proc Natl Acad Sci U S A 104: 14519-14524.
7. Kiyasova V, Fernandez SP, Laine J, Stankovski L, Muzerelle A, et al. (2011) A Genetically Defined Morphologically and Functionally Unique Subset of 5-HT Neurons in the Mouse Raphe Nuclei. J Neurosci 31: 2756-2768.
8. Hodges MR, Wehner M, Aungst J, Smith JC, Richerson GB, (2009) Transgenic mice lacking serotonin neurons have severe apnea and high mortality during development. J Neurosci 29: 10341-10349.
9. Dai JX, Han HL, Tian M, Cao J, Xiu JB, et al. (2008) Enhanced contextual fear memory in central serotonin-deficient mice. Proc Natl Acad Sci U S A 105: 11981-11986.
10. Song NN, Xiu JB, Huang Y, Chen JY, Zhang L, et al. (2011) Adult raphe-specific deletion of Lmx1b leads to central serotonin deficiency. PLoS One 6: e15998.
11. Audero E, Coppi E, Mlinar B, Rossetti T, Caprioli A, et al. (2008) Sporadic autonomic dysregulation and death associated with excessive serotonin autoinhibition. Science 321: 130-133.
12. Buehr M, Meek S, Blair K, Yang J, Ure J, et al. (2008) Capture of authentic embryonic stem cells from rat blastocysts. Cell 135: 1287-1298.
13. Homberg JR, Olivier JD, Smits BM, Mul JD, Mudde J, et al. (2007) Characterization of the serotonin transporter knockout rat: a selective change in the functioning of the serotonergic system. Neuroscience 146: 1662-1676.
14. Sato Y, Endo H, Ajiki T, Hakamata Y, Okada T, et al. (2004) Establishment of Cre/LoxP recombination system in transgenic rats. Biochem Biophys Res Commun 319: 1197-1202.
15. Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, et al. (2009) Knockout rats via embryo microinjection of zinc-finger nucleases. Science 325: 433.
16. Weber T, Bohm G, Hermann E, Schutz G, Schönig K, et al. (2009) Inducible gene manipulations in serotonergic neurons. Front Mol Neurosci 2: 24.
17. Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, et al. (2001) Green fluorescent protein-transgenic rat: a tool for organ transplantation research. Biochem Biophys Res Commun 286: 779-785.
18. Michalkiewicz M, Michalkiewicz T, Geurts AM, Roman RJ, Slocum GR, et al. (2007) Efficient transgenic rat production by a lentiviral vector. Am J Physiol Heart Circ Physiol 293: H881-894.
19. Schönig K, Kentner D, Gossen M, Baldinger T, Miao J, et al. (2011) Development of a BAC vector for integration-independent and tight regulation of transgenes in rodents via the Tet system. Transgenic Res 20: 709-720.
20. Giraldo P, Montoliu L, (2001) Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res 10: 83-103.
21. Niwa H, Yamamura K, Miyazaki J, (1991) Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108: 193-199.
22. Walther DJ, Peter JU, Bashammakh S, Hortnagl H, Voits M, et al. (2003) Synthesis of serotonin by a second tryptophan hydroxylase isoform. Science 299: 76.
23. Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, et al. (2003) A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425: 917-925.
24. Chandler KJ, Chandler RL, Broeckelmann EM, Hou Y, Southard-Smith EM, et al. (2007) Relevance of BAC transgene copy number in mice: transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression. Mamm Genome 18: 693-708.
25. Leone DP, Genoud S, Atanasoski S, Grausenburger R, Berger P, et al. (2003) Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells. Mol Cell Neurosci 22: 430-440.
26. Lagace DC, Whitman MC, Noonan MA, Ables JL, DeCarolis NA, et al. (2007) Dynamic contribution of nestin-expressing stem cells to adult neurogenesis. J Neurosci 27: 12623-12629.
27. Mori T, Tanaka K, Buffo A, Wurst W, Kuhn R, et al. (2006) Inducible gene deletion in astroglia and radial glia-a valuable tool for functional and lineage analysis. Glia 54: 21-34.
28. Jacob HJ, Lazar J, Dwinell MR, Moreno C, Geurts AM, (2010) Gene targeting in the rat: advances and opportunities. Trends Genet 26: 510-518.
29. Li MA, Bradley A, (2011) Crafting rat genomes with zinc fingers. Nat Biotechnol 29: 39-41.
30. Cui X, Ji D, Fisher DA, Wu Y, Briner DM, et al. (2010) Targeted integration in rat and mouse embryos with zinc-finger nucleases. Nat Biotechnol 29: 64-67.
31. Li T, Huang S, Zhao X, Wright DA, Carpenter S, et al. (2011) Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes. Nucleic Acids Res.
32. Zan Y, Haag JD, Chen KS, Shepel LA, Wigington D, et al. (2003) Production of knockout rats using ENU mutagenesis and a yeast-based screening assay. Nat Biotechnol 21: 645-651.
33. Smits BM, Mudde JB, van de Belt J, Verheul M, Olivier J, et al. (2006) Generation of gene knockouts and mutant models in the laboratory rat by ENU-driven target-selected mutagenesis. Pharmacogenet Genomics 16: 159-169.
34. Izsvak Z, Frohlich J, Grabundzija I, Shirley JR, Powell HM, et al. (2010) Generating knockout rats by transposon mutagenesis in spermatogonial stem cells. Nat Methods 7: 443-445.
35. Brown BD, Naldini L, (2009) Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications. Nat Rev Genet 10: 578-585.
36. Ebert MS, Neilson JR, Sharp PA, (2007) MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods 4: 721-726.
37. Boyden ES, Zhang F, Bamberg E, Nagel G, Deisseroth K, (2005) Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci 8: 1263-1268.
38. Deisseroth K, Feng G, Majewska AK, Miesenbock G, Ting A, et al. (2006) Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci 26: 10380-10386.
39. Scott MM, Wylie CJ, Lerch JK, Murphy R, Lobur K, et al. (2005) A genetic approach to access serotonin neurons for in vivo and in vitro studies. Proc Natl Acad Sci U S A 102: 16472-16477.
40. Hodges MR, Tattersall GJ, Harris MB, McEvoy SD, Richerson DN, et al. (2008) Defects in breathing and thermoregulation in mice with near-complete absence of central serotonin neurons. J Neurosci 28: 2495-2505.
41. Buchanan GF, Richerson GB, (2010) Central serotonin neurons are required for arousal to CO2. Proc Natl Acad Sci U S A 107: 16354-16359.
42. Zhao ZQ, Chiechio S, Sun YG, Zhang KH, Zhao CS, et al. (2007a) Mice lacking central serotonergic neurons show enhanced inflammatory pain and an impaired analgesic response to antidepressant drugs. J Neurosci 27: 6045-6053.
43. Kim JC, Cook MN, Carey MR, Shen C, Regehr WG, et al. (2009) Linking genetically defined neurons to behavior through a broadly applicable silencing allele. Neuron 63: 305-315.
44. Dayan P, Huys QJ, (2009) Serotonin in affective control. Annu Rev Neurosci 32: 95-126.
45. Rogers RD, (2011) The roles of dopamine and serotonin in decision making: evidence from pharmacological experiments in humans. Neuropsychopharmacology 36: 114-132.
46. Aitman TJ, Critser JK, Cuppen E, Dominiczak A, Fernandez-Suarez XM, et al. (2008) Progress and prospects in rat genetics: a community view. Nat Genet 40: 516-522.