Relevance of BAC transgene copy number in mice: Transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression

Mammalian Genome

Volumn 18, Issue 10, 2007, Pages 693-708

  Chandler, Kelly J ^a   Chandler, Ronald L ^a   Broeckelmann, Eva M ^a   Hou, Yue ^a   Southard Smith, E Michelle ^b   Mortlock, Douglas P ^a  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CONTROLLED STUDY; GENE EXPRESSION; GENE FUNCTION; GENE REPLICATION; GENETIC ANALYSIS; MOUSE; NONHUMAN; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSGENE; TRANSGENICS;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DNA; GENE EXPRESSION; GENETIC TECHNIQUES; MICE; MICE, TRANSGENIC; MODELS, GENETIC; NUCLEIC ACID HYBRIDIZATION; PEDIGREE; POLYMORPHISM, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEPHAROSE; TRANSGENES;

BACTERIA (MICROORGANISMS); MUS; MUS MUSCULUS;

EID: 36148952528     PISSN: 09388990     EISSN: 14321777     Source Type: Journal    
DOI: 10.1007/s00335-007-9056-y     Document Type: Article

References (24)

1. Alexander GM, Erwin KL, Byers N, Deitch JS, Augelli BJ, et al. (2004) Effect of transgene copy number on survival in the G93A SOD1 transgenic mouse model of ALS. Brain Res Mol Brain Res 130:7-15
2. Ballester M, Castello A, Ibanez E, Sanchez A, Folch JM (2004) Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals. Biotechniques 37:610-613
3. Bishop J (1996) Chromosomal insertion of foreign DNA. Reprod Nutr Dev 36:607-618
4. Bishop JO, Smith P (1989) Mechanism of chromosomal integration of microinjected DNA. Mol Biol Med 6:283-298
5. Bitgood MJ, McMahon AP (1995) Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo. Dev Biol 172:126-138
6. Burkhart CA, Norris MD, Haber M (2002) A simple method for the isolation of genomic DNA from mouse tail free of real-time PCR inhibitors. J Biochem Biophys Methods 52:145-149
7. Chandler RL, Chandler KJ, McFarland KA, Mortlock DP (2007) Bmp2 transcription in osteoblast progenitors is regulated by a distant 3′ enhancer located 156.3 kilobases from the promoter. Mol Cell Biol 27:2934-2951
8. Copeland NG, Jenkins NA, Court DL (2001) Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet 2:769-779
9. Deal KK, Cantrell VA, Chandler RL, Saunders TL, Mortlock DP, et al. (2006) Distant regulatory elements in a Sox10-beta GEO BAC transgene are required for expression of Sox10 in the enteric nervous system and other neural crest-derived tissues. Dev Dyn 235:1413-1432
10. DiLeone RJ, Russell LB, Kingsley DM (1998) An extensive 3′ regulatory region controls expression of Bmp5 in specific anatomical structures of the mouse embryo. Genetics 148:401-408
11. Furuta Y, Piston DW, Hogan BL (1997) Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development. Development 124:2203-2212
12. Giraldo P, Montoliu L (2001) Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res 10:83-103
13. Giraldo P, Rival-Gervier S, Houdebine LM, Montoliu L (2003) The potential benefits of insulators on heterologous constructs in transgenic animals. Transgenic Res 12:751-755
14. Gong S, Zheng C, Doughty ML, Losos K, Didkovsky N, et al. (2003) A gene expression atlas of the central nervous system based on bacterial artificial chromosomes. Nature 425:917-925
15. Hamada T, Sasaki H, Seki R, Sakaki Y (1993) Mechanism of chromosomal integration of transgenes in microinjected mouse eggs: sequence analysis of genome-transgene and transgene-transgene junctions at two loci. Gene 128:197-202
16. Heaney JD, Bronson SK (2006) Artificial chromosome-based transgenes in the study of genome function. Mamm Genome 17:791-807
17. Heintz N (2001) BAC to the future: the use of bac transgenic mice for neuroscience research. Nat Rev Neurosci 2:861-870
18. Jaenisch R (1988) Transgenic animals. Science 240:1468-1474
19. Jones CM, Lyons KM, Hogan BL (1991) Involvement of Bone Morphogenetic Protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse. Development 111:531-542
20. Lee EC, Yu D, Martinez de Velasco J, Tessarollo L, Swing DA, et al. (2001) A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73:56-65
21. Li S, Hammer RE, George-Raizen JB, Meyers KC, Garrard WT (2000) High-level rearrangement and transcription of yeast artificial chromosome-based mouse Ig kappa transgenes containing distal regions of the contig. J Immunol 164:812-824
22. Liu W, Sun X, Braut A, Mishina Y, Behringer RR, et al. (2005) Distinct functions for Bmp signaling in lip and palate fusion in mice. Development 132:1453-1461
23. Mortlock DP, Guenther C, Kingsley DM (2003) A general approach for identifying distant regulatory elements applied to the Gdf6 gene. Genome Res 13:2069-2081
24. Tesson L, Heslan JM, Menoret S, Anegon I (2002) Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Res 11:43-48