Production of knockout rats using ENU mutagenesis and a yeast-based screening assay

Nature Biotechnology

Volumn 21, Issue 6, 2003, Pages 645-651

  Zan, Yunhong ^a   Haag, Jill D ^a   Chen, Kai Shun ^a   Shepel, Laurie A ^a   Wigington, Don ^a   Wang, Yu Rong ^a   Hu, Rong ^a   Lopez Guajardo, Christine C ^a   Brosel, Heidi L ^a   Porter, Katherine I ^a   Leonard, Rachel A ^a   Hitt, Andrew A ^a   Schommer, Stacy L ^a   Elegbede, Anu F ^a   Gould, Michael N ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMEDICAL ENGINEERING; GENES; TUMORS; YEAST;

KNOCKOUT RATS;

BIOTECHNOLOGY;

BRCA1 PROTEIN; BRCA2 PROTEIN; CHIMERIC PROTEIN; ETHYLNITROSOUREA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BREAST CANCER; CONTROLLED STUDY; GENE DISRUPTION; GERM LINE; HERITABILITY; KNOCKOUT RAT; MALE; MALE FERTILITY; MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; RAT; RAT STRAIN; REPORTER GENE; SUPPRESSOR GENE; YEAST;

ANIMALS; BRCA1 PROTEIN; BRCA2 PROTEIN; CELLS, CULTURED; ETHYLNITROSOUREA; GENE EXPRESSION REGULATION; GENE TARGETING; MALE; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RATS; RATS, MUTANT STRAINS; RATS, SPRAGUE-DAWLEY; RECOMBINANT PROTEINS; YEASTS;

ANIMALIA; RODENTIA;

EID: 0037685273     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt830     Document Type: Article

References (20)

1. Jacob, H.J. & Kwitek, A.E. Rat genetics: attaching physiology and pharmacology to the genome. Nat. Rev. Genet. 3, 33-42 (2002).
2. Justice, M.J., Noveroske, J.K., Weber, J.S., Zheng, B. & Braduley, A. Mouse ENU mutagenesis. Hum. Mol. Genet. 8, 1955-1963 (1999).
3. Noveroske, J.K., Weber, J.S. & Justice, M.J. The mutagenic action of N-ethyl-Nnitrosourea in the mouse. Mamm. Genome 11, 478-483 (2000).
4. Russell, W.L. et al. Specific-locus test shows ethylnitrosourea to be the most potent mutagen in the mouse. Proc. Natl. Acad. Sci. USA 76, 5918-5922 (1979).
5. Beier, D.R. Sequence-based analysis of mutagenized mice. Mamm. Genome 11, 594-597 (2000).
6. Justice, M.J. et al. Effects of ENU dosage on mouse strains. Mamm. Genome 11, 484-488 (2000).
7. Ishioka, C. et al. Screening patients for heterozygous p53 mutations using a functional assay in yeast. Nat. Genet. 5, 124-129 (1993).
8. Kataoka, A. et al. Development of a yeast stop codon assay readily and generally applicable to human genes. Am. J. Pathol. 159, 1239-1245 (2001).
9. Culbertson, M.R. RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer. Trends Genet. 15, 74-80 (1999).
10. Frischmeyer, P.A. & Dietz, H.C. Nonsense-mediated mRNA decay in health and disease. Hum. Mol. Genet. 8, 1893-1900 (1999).
11. Kuramoto, T., Nomoto, T., Sugimura, T. & Ushijima, T. Cloning of the rat agouti gene and identification of the rat nonagouti mutation. Mamm. Genome 12, 469-471 (2001).
12. Suzuki, A. et al. Brca2 is required for embryonic cellular proliferation in the mouse. Genes Dev. 11, 1242-1252 (1997).
13. Connor, F. et al. Tumorigenesis and a DNA repair defect in mice with a truncating Brca2 mutation. Nat. Genet. 17, 423-430 (1997).
14. Freidman, L.S. et al. Thymic lymphomas in mice with a truncating mutation in Brca2. Cancer Res. 58, 1338-1343 (1998).
15. Flaman, J.-M. et al. A simple p53 functional assay for screening cell lines, blood, and tumors. Proc. Natl. Acad. Sci. USA 92, 3963-3967 (1995).
16. Andreutti-Zaugg, C., Scott, R.J. & Iggo, R. Inhibition of nonsense-mediated messenger RNA decay in clinical samples facilitates detection of human MSH2 mutations with an in vivo fusion protein assay and conventional techniques. Cancer Res. 57, 3288-3293 (1997).
17. Nakatsukasa, E. et al. Generation of live rat offspring by intrauterine insemination with epididymal spermatozoa cryopreserved at -196 °C. Reproduction 122, 463-467 (2001).
18. Nakagata, N. Cryopreservation of mouse spermatozoa. Mamm. Genome 11, 572-576 (2000).
19. Coghill, E.L. et al. A gene-driven approach to the identification of ENU mutants in the mouse. Nat. Genet. 30, 255-256 (2002).
20. Yamamoto, K. et al. A functional and quantitative mutational analysis of p53 mutations in yeast indicates strand biases and different roles of mutations in DMBA- and BBN-induced tumors in rats. Int. J. Cancer 83, 700-705 (1999).