Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers

BioTechniques

Volumn 44, Issue 7, 2008, Pages 924-926

  Vroom, Jonathan A ^a   Wang, Clifford L ^a  

^a Stanford University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LIGASE; OLIGONUCLEOTIDE; PLASMID DNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; MOLECULAR RECOGNITION; NUCLEOTIDE SEQUENCE; PLASMID; TECHNIQUE;

BASE SEQUENCE; CLONING, MOLECULAR; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PLASMIDS;

EID: 47549095527     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112808     Document Type: Article

References (6)

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2. Aslanidis, C. and P.J. de Jong. 1990. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18:6069-6074.
3. Zhu, B., G. Cai, E.O. Hall, and G.J. Freeman. 2007. In-fusion assembly: seam-less engineering of multidomain fusion proteins, modular vectors, and mutations. BioTechniques 43:354-359.
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6. Smith, H.O., C.A. Hutchison III, C. Pfannkoch, and J.C. Venter. 2003. Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides. Proc. Natl. Acad. Sci. USA 100:15440-15445.