Dual salt precipitation for the recovery of a recombinant protein from Escherichia coli

Biotechnology Progress

Volumn 27, Issue 5, 2011, Pages 1306-1314

  Balasundaram, Bangaru ^a   Sachdeva, Soam ^a   Bracewell, Daniel G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Dual salt precipitation; Fab; Primary recovery; Scale down centrifugation; Synergistic effects

Indexed keywords

ALTERNATIVE PROCESS; AMMONIUM SULFATE; ANTAGONISTIC EFFECTS; BIOPHARMACEUTICALS; CITRATE IONS; CLARIFICATION PERFORMANCE; FAB; INTRACELLULAR PROTEINS; PRECIPITATING AGENTS; RECOMBINANT PROTEIN; SALT PRECIPITATION; SALTING-OUT EFFECT; SCALE-DOWN; SODIUM CITRATE; SOLUBILITY STUDIES; SYNERGISTIC EFFECT;

AMMONIUM COMPOUNDS; CENTRIFUGATION; CENTRIFUGES; CLARIFIERS; ESCHERICHIA COLI; IMPURITIES; PROTEINS; RECOVERY; SODIUM SULFATE; SOLUBILITY;

PRECIPITATION (CHEMICAL);

IMMUNOGLOBULIN F(AB) FRAGMENT; INORGANIC SALT; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; PARTICLE SIZE; PRECIPITATION; SOLUBILITY; STATIC ELECTRICITY;

CHEMICAL PRECIPITATION; ESCHERICHIA COLI; IMMUNOGLOBULIN FAB FRAGMENTS; PARTICLE SIZE; RECOMBINANT PROTEINS; SALTS; SOLUBILITY; STATIC ELECTRICITY;

ESCHERICHIA COLI;

EID: 80053929111     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.645     Document Type: Article

References (31)

1. Ferrer-Miralles N, Domingo-Espín J, Corchero JL, Vázquez E, Villaverde A. Microbial factories for recombinant pharmaceuticals. Microb Cell Fact. 2009; 8: 17-25.
2. Andersen DC, Reilly DE. Production technologies for monoclonal antibodies and their fragments. Curr Opin Biotechnol. 2004; 15: 456-462.
3. Przybycien TM, Pujar NS, Steele LM. Alternative bioseparation operations: life beyond packed-bed chromatography. Curr Opin Biotechnol. 2004; 15: 469-478.
4. Low D, O'Leary R, Pujar NS. Future of antibody purification. J Chrom B. 2007; 848: 48-63.
5. Mohanty JG, Elazhary Y. Purification of IgG from serum with caprylic acid and ammonium sulfate precipitation is not superior to ammonium sulfate precipitation alone. Comp lmmun Microb Inject Dis. 1989; 12: 153-160.
6. McDonald P, Victa C, Carter-Franklin JN, Fahrner R. Selective antibody precipitation using polyelectrolytes: a novel approach to the purification of monoclonal antibodies. Biotechnol Bioeng. 2009; 102: 1141-1151.
7. Venkiteshwaran A, Heider P, Teysseyre L, Belfort G. Selective precipitation-assisted recovery of immunoglobulins from bovine serum using controlled-fouling crossflow membrane microfiltration. Biotechnol Bioeng. 2008; 101: 957-966.
8. Persson J, Lester P. Purification of antibody and antibody-fragment from E. coli homogenate using 6, 9-diamino-2-ethoxyacridine lactate as precipitation agent. Biotechnol Bioeng. 2004; 87: 424-434.
9. Richardson P, Hoare M, Dunnill P. A new biochemical engineering approach to the fractional precipitation of proteins. Biotechnol Bioeng. 1990; 36: 354-366.
10. Tsoka S, Ciniawskyj OC, Thomas OR, Titchener-Hooker NJ, Hoare M. Selective flocculation and precipitation for the improvement of virus-like particle recovery from yeast homogenate. Biotechnol Prog. 2000; 16: 661-667.
11. Hunter AK, Wang X, Suda EJ, Herberg JT, Shell RE, Thomas KE, Dufield RL, Gustafson ME, Mozier NM, Ho SV. Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I. Biotechnol Prog. 2009; 25: 446-453.
12. Senczuk AM, Klinke R, Arakawa T, Vedantham G, Yigzaw Y. Hydrophobic interaction chromatography in dual salt system increases protein binding capacity. Biotechnol Bioeng. 2009; 103: 930-935.
13. Tustian AD, Salte H, Willoughby N, Hassan I, Rose MH, Baganz F, Hoare M, Titchener-Hooker NJ. Adapted ultra scale-down approach for predicting the centrifugal separation behavior of high cell density cultures. Biotechnol Prog. 2007; 23: 1404-1410.
14. Foster PR, Dunnill P, Lilly MD. The kinetics of protein salting-out: precipitation of yeast enzymes by ammonium sulfate. Biotechnol Bioeng. 1976; 18: 545-580.
15. Butler M, Montgomery H. The solubility of the plasma proteins: I. Dependence of salt and plasma concentrations in concentrated solutions of potassium phosphate. J Biol Chem. 1932; 99: 173-195.
16. Burns DB, Zydney AL. Buffer effects on the zeta potential of ultrafiltration membranes. J Memb Sci. 2000; 172: 39-48.
17. Salte H, King JMP, Baganz F, Hoare M, Titchener-Hooker NJ. A methodology for centrifuge selection for the separation of high solids density cell broths by visualization of performance using windows of operation. Biotechnol Bioeng. 2006; 95: 1218-1227.
18. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng. 2006; 95: 483-491.
19. Boychyn M, Yim SSS, Bulmer M, More J, Bracewell D, Hoare M. Performance prediction of industrial centrifuges using scale-down models. Bioprocess Biosyst Eng. 2004; 26: 385-391.
20. Boychyn M, Doyle W, Bulmer M, More J, Hoare M. Laboratory scale-down of protein purification processes involving fractional precipitation and centrifugal recovery. Biotechnol Bioeng. 2000; 69: 1-10.
21. Schoengen A, Schreiner T, Anselstetter V, Binder T, Galle J, Weber L, Heimpel H. Cryocrystalglobulinemia: pH-dependent precipitation of a monoclonal IgG-kappa-immunoglobulin. Blut. 1989; 58: 255-260.
22. Balasundaram B, Nesbeth D, Ward JM, Bracewell DG. Step change in the efficiency of centrifugation through cell engineering: co-expression of Staphylococcal nuclease to reduce the viscosity of the bioprocess feedstock. Biotechnol Bioeng. 2009; 104: 134-142.
23. Berrill A, Ho SV, Bracewell DG. Ultra scale-down to define and improve the relationship between flocculation and disc-stack centrifugation. Biotechnol Prog. 2008; 24: 426-431.
24. Neal G, Christie J, Ayazi-Shamlou P, Keshavarz-Moore E. An ultra scale-down approach for the prediction of full-scale recovery of ovine polyclonal immunoglobulins used in the manufacture of snake venom-specific Fab' fragment. Biotechnol Bioeng. 2003; 81: 149-157.
25. Zaman F, Allan CM, Ho SV. Ultra scale-down approaches for clarification of mammalian cell culture broths in disc-stack centrifuges. Biotechnol Prog. 2009; 25: 1709-1716.
26. Clarkson AI, Bulmer M, Titchener-Hooker NJ. Pilot-scale verification of a computer-based simulation for the centrifugal recovery of biological particles. Bioprocess Eng. 1996; 14: 81-89.
27. Melander W, Horvath C. Salt effects on hydropobic interactions in precipitation and chromatography of proteins: an interpretation of the lyotropic sereies. Arch Biochem Biophys. 1977; 183: 200-215.
28. Harinarayan C, Skidmore K, Kao Y, Zydney L, van Reis R. Small molecule clearance in ultrafiltration/diafiltration in relation to protein interactions: study of citrate binding to a Fab. Biotechnol Bioeng. 2009; 102: 1718-1722.
29. Shih YC, Prausnitz JM, Blanch HW. Some characteristics of protein precipitation by salts. Biotechnol Bioeng. 1992; 40: 1155-1164.
30. Iyer HV, Przybycien TM. Protein precipitation: effects of mixing on protein solubility. AIChE J. 1994; 40: 349-360.
31. Kent UM. Purification of antibodies using ammonium sulfate fractionation or gel filtration. Immunocytochemical methods and protocols. Methods Mol Biol. 1999; 34: 13-21.