Step change in the efficiency of centrifugation through cell engineering: Co-expression of Staphylococcal nuclease to reduce the viscosity of the bioprocess feedstock

Biotechnology and Bioengineering

Volumn 104, Issue 1, 2009, Pages 134-142

  Balasundaram, B ^a   Nesbeth, D ^a   Ward, J M ^a   Keshavarz Moore, E ^a   Bracewell, D G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Cell disruption; Cell engineering; Clarification; Nuclease; Viscosity

Indexed keywords

BIOPROCESSES; BIOPROCESSINGS; CELL DEBRIS; CELL DISRUPTION; CELL ENGINEERING; CLARIFICATION PERFORMANCE; CO-EXPRESSION; DOWNSTREAM-PROCESSING; E. COLI; LABORATORY SCALE; NUCLEASE; PROCESS STREAMS; RECOMBINANT PROTEIN; SCALE-DOWN; STAPHYLOCOCCAL NUCLEASE; STAPHYLOCOCCUS AUREUS; STEP CHANGES; VISCOSITY REDUCTION;

CENTRIFUGATION; CENTRIFUGES; CLARIFICATION; CLARIFIERS; DEBRIS; ESCHERICHIA COLI; NUCLEIC ACIDS; VISCOSITY;

CELL MEMBRANES;

NUCLEASE; NUCLEIC ACID;

ARTICLE; BACTERIAL STRAIN; BIOMASS; BIOPROCESS; CELL SIZE; CELLULAR DISTRIBUTION; CENTRIFUGATION; CONTROLLED STUDY; ESCHERICHIA COLI; NONHUMAN; PLASMID; PROTEIN EXPRESSION; STAPHYLOCOCCUS AUREUS; VISCOSITY;

CENTRIFUGATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MICROCOCCAL NUCLEASE; RECOMBINANT PROTEINS; VISCOSITY;

ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS;

EID: 67650692398     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22369     Document Type: Article

References (45)

1. Agerkvist I, Enfors S-O. 1990. Characterization of E. coli cell disintegrates from a bead mill and high pressure homogenizers. Biotechnol Bioeng 36:1083-1089.
2. Allen T. 1975. Particle size measurement. London: Chapman and Hall Ltd, p 99.
3. Andersen DC, Reilly DE. 2004. Production technologies for monoclonal antibodies and their fragments. Curr Opin Biotechnol 15:456-462. (Pubitemid 39304044)
4. Anfinsen CB, Cuatrecasas P, Taniuchi H. 1971. Staphylococcal nuclease, chemical properties and catalysis. In: Boyer P, editor. The enzymes, Vol.4. New York: Academic Press, p 177-204.
5. Anspach FB, Curbelo D, Hartmann R, Garke G, Deckwer WD. 1999. Expanded-bed chromatography in primary protein purification. J Chromatogr A 865:129-144.
6. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, editors. 1987. Current protocols in molecular biology. New York: Greene Publishing Associates and Wiley-Interscience, 2.1.3-2.1.4, 15.3.8-15.3.11p.
7. Bachmann BJ. 1996. Derivatives and genotypes of some mutant derivatives of Escherichia coli K-12. In: Neidhardt FC, et al. editors. Escherichia coli and Salmonella: Cellular and molecular biology, 2nd edn. Washington: ASM Press, p 2460-2488.
8. Bailey SM, Meagher MM. 1997. Crossflow microfiltration of recombinant Escherichia coli lysates after high pressure homogenization. Biotechnol Bioeng 56:304-310.
9. Bailey SM, Blum P, Meagher MM. 1995. Improved homogenization of recombinant Escherichia coli following pretreatment with guanidine hydrochloride. Biotechnol Prog 11:533-539.
10. Ball TK, Saurugger PN, Benedik MJ. 1987. The extracellular nuclease gene of Serratia marcescens and its secretion from Escherichia coli. Gene 57:183-192.
11. Boychyn M, Doyle W, Bulmer M, More J, Hoare M. 2000. Laboratory scaledown of protein purification processes involving fractional precipitation and centrifugal recovery. Biotechnol Bioeng 69:1-10. (Pubitemid 30418256)
12. Boychyn M, Yim SSS, Bulmer M, More J, Bracewell DG, Hoare M. 2004. Performance prediction of industrial centrifuges using scale-down models. Bioprocess Biosyst Eng 26:385-391.
13. Bracewell DG, Boychyn M, Baldascini H, Storey SA, Bulmer M, More J, Hoare M. 2008. Impact of clarification strategy on chromatographic separations: Pre-processing of cell homogenates. Biotechnol Bioeng 100:941-949.
14. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
15. Ciccolini LAS, Ayazi Shamlou P, Titchener-Hooker NJ, Ward JM, Dunnill P. 1999. Rheological properties of chromosomal and plasmid DNA during alkaline lysis reaction. Bioprocess Eng 21:231-237. (Pubitemid 29439076)
16. Cooke GD, Cranenburgh RM, Hanak JAJ, Ward JM. 2003. A modified Escherichia coli protein production strain expressing staphylococcal nuclease, capable of auto-hydrolysing host nucleic acid. J Biotechnol 101:229-239.
17. Datar R, Rosen CG. 1987. Centrifugal separation in the recovery of intracellular protein from E. coli. Chem Eng J 34:B49-B56. (Pubitemid 17572199)
18. Enfors S-O, Hellebust H, Köhler K, Strandberg L, Veide A. 1990. Impact of genetic engineering on downstream processing of proteins produced in E. coli. Adv Biochem Eng/Biotechnol 43:31-42.
19. Fane AG, Radovich GM. 1990. Membrane systems. In: Asenjo JA, editor. Separation processes in biotechnology. New York; Basel: Marcel Dekker, Inc., p 209-262.
20. Frej AKB, Hjorth R, Hammarstrom A. 1994. Pilot scale recovery of recombinant annexin V from unclarified Escherichia coli homogenate using expanded bed adsorption. Biotechnol Bioeng 44:922. (Pubitemid 24308540)
21. Furste JP, Pansegrau W, Frank R, Blcker H, Scholz P, Bagdasarian M, Lanka E. 1986. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131. (Pubitemid 17006084)
22. Grabski A, McCormick M, Mierendorf O. 1999. BugBuster™ and Benzonase®: The clear solutions to simple efficient extraction of E. coli proteins. Innovations 10:17-19.
23. Hagen AJ, Oliver CN, Sitrin RD. 1996. Optimization of poly(ethylene glycol) precipitation of hepatitis A virus used to prepare a highly purified inactivated vaccine. Biotechnol Prog 12:406-412.
24. He YB, Laskowski JS, Klein B. 2001. Particle movement in non-Newtonian slurries: The effect of yield stress on dense medium separation. Chem Eng Sci 56:2991-2998. (Pubitemid 32548519)
25. Hoare M. 1982. Protein precipitation and precipitate ageing. Trans Inst Chem Eng 60:79-87.
26. Humphreys DP, Heywood SP, King LM, Bowering LC, Turner JP, Lane SE. 2004. Engineering of Escherichia coli to improve the purification of periplasmic Fab? fragments: Changing the pI of the chromosomally encoded PhoS/PstS protein. Protein Expression Purif 37:109-118.
27. Hutchinson N, Bingham N, Murrell N, Farid S, Hoare M. 2006. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol Bioeng 95:483-491. (Pubitemid 44495383)
28. Huyghe BG, Liu X, Sutjipto S, Sugarman BJ, Horn MT, Shepard HM, Scandella CJ, Shabram P. 1995. Purification of a type 5 recombinant adenovirus encoding human p53 by column chromatography. Human Gene Ther 6:1403-1416.
29. Keshavarz E. 1991. New findings on fundamentals of cell disruption in high pressure homogenisers. In: White MD, Reuveny S, Shafferman A, editors. Biologicals from recombinant microorganisms and animal cells production and recovery. Weinheim: Balaban publishers, p 277-292.
30. Keshavarz E, Bonnerjea J, Hoare M, Dunnill P. 1990. Disruption of a fungal organism, Rhizopus nigricans, in a high-pressure homogenizer. Enz Microb Technol 12:494-498. (Pubitemid 20331658)
31. Kleinig AR, Mansell CJ, Nguyen QD, Badalyan A, Middelberg APJ. 1995. Influence of broth dilution on the disruption of Escherichia coli. Biotechol Tech 9:759-762.
32. Lee CT, Morreale G, Middelberg APJ. 2004. Combined in-fermenter extraction and cross-flow microfiltration for improved inclusion body processing. Biotechnol Bioeng 85:103-113. (Pubitemid 38090135)
33. Leung WS, Swartz JR. 2003. Process for recovering heterologous polypeptides from bacterial cells. EP19990971031.
34. Low D, O'Leary R, Pujar NS. 2007. Future of antibody purification. J Chromatogr B 848:48-63.
35. Neal G, Christie J, Ayazi-Shamlou P, Keshavarz-Moore E. 2003. An ultra scale-down approach for the prediction of full-scale recovery of ovine polyclonal immunoglobulins used in the manufacture of snake venomspecific Fab? fragment. Biotechnol Bioeng 81:149-157. (Pubitemid 36077291)
36. Przybycien TM, Pujar NS, Steele LM. 2004. Alternative bioseparation operations: Life beyond packed-bed chromatography. Curr Opin Biotechnol 15:469-478.
37. Rawlings DE, Tietze E. 2001. Comparative biology of IncQ and IncQ-like plasmids. Microbiol Mol Biol Rev 65:481-496. (Pubitemid 33123515)
38. Salte H, King JM, Baganz F, Hoare M, Titchener-Hooker NJ. 2006. A methodology for centrifuge selection for the separation of high solids density cell broths by visualisation of performance using windows of operation. Biotechnol Bioeng 95:1218-1227. (Pubitemid 44901337)
39. Shu-Sen W. 1988. Effect of solution viscosity on ultrafiltration flux. J Membr Sci 39:187-194.
40. Siddiqi SF, Titchener-Hooker NJ, Shamlou A. 1996. Simulation of particle size distribution changes occurring during high-pressure disruption of bakers? yeast. Biotechnol Bioeng 50:145-150. (Pubitemid 26095148)
41. Takahara M, Hibler DW, Barr PJ, Gerlt JA, Inouye M. 1985. The ompA signal peptide directed secretion of Staphylococcal nuclease A by Escherichia coli. J Biol Chem 260:2670-2674. (Pubitemid 15139319)
42. Tarleton ES, Wakeman RJ. 1994. Understanding flux decline in crossflow microfiltration:Part II-Effects of process parameters. Chem Eng Res Des 72:431-440.
43. Tran R, Joseph JR, Sinclair A, Bracewell DG, Zhou Y, Titchener-Hooker NJ. 2007. A framework for the prediction of scale-up when using compressible chromatographic packings. Biotechnol Prog 23:413-422. (Pubitemid 46626316)
44. Tustian AD, Salte H, Willoughby N, Hassan I, Rose M, Baganz F, Hoare M, Titchener-Hooker NJ. 2007. Adapted ultra scale-down approach for predicting the centrifugal separation behavior of high cell density cultures. Biotechnol Prog 23:1404-1410. (Pubitemid 350261876)
45. Zhang J, Fang H, Dai H, Xie D, Chen H. 2008. Constructing modified protein-producing Escherichia coli capable of autohydrolysing host nucleic acid during cell lysis. Chinese J Biotech 24:46-52. (Pubitemid 351215158)