Selective precipitation-assisted recovery of immunoglobulins from bovine serum using controlled-fouling crossflow membrane microfiltration

Biotechnology and Bioengineering

Volumn 101, Issue 5, 2008, Pages 957-966

  Venkiteshwaran, Adith ^a   Heider, Patrick ^a   Teysseyre, Laure ^a   Belfort, Georges ^a  

^a Rensselaer Polytechnic Institute   (United States)

Author keywords

Concentration polarization; Crossflow microfiltration; Fouling; IgG recovery; Salt precipitation; Shear rate

Indexed keywords

AFFINITY CHROMATOGRAPHY; AGGLOMERATION; AMMONIUM COMPOUNDS; ANTIGENS; BIOCHEMICAL ENGINEERING; CELL CULTURE; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; CONCENTRATION (PROCESS); FOULING; MEMBRANES; MICROFILTRATION; PRECIPITATION (CHEMICAL); REYNOLDS NUMBER; SALT REMOVAL;

AMMONIUM SULFATES; ANION EXCHANGES; BIOLOGICAL FLUIDS; BOVINE SERUM ALBUMINS; BOVINE SERUMS; CELL LYSATE; CONCENTRATION POLARIZATION; CROSSFLOW MEMBRANES; CROSSFLOW MICROFILTRATION; CULTURE SUPERNATANT; DIAFILTRATION; ECONOMIC RECOVERIES; FLOW DISTRIBUTIONS; HIGH SELECTIVITIES; HYBRID METHODS; IGG RECOVERY; IMMUNOGLOBULIN G; MEMBRANE CHROMATOGRAPHIES; MEMBRANE MICROFILTRATION; MEMBRANE PORE SIZES; PERMEATE FLUXES; PRECIPITATION PROCESSES; PROTEIN A; RECIRCULATION RATES; RESOLUBILIZATION; SCALE-UP; SELECTIVE PRECIPITATIONS; SELECTIVE REMOVALS; SHEAR RATE;

BODY FLUIDS;

AMMONIUM SULFATE; BOVINE SERUM ALBUMIN; IMMUNOGLOBULIN; IMMUNOGLOBULIN G; PROTEIN A;

ADSORPTION; AFFINITY CHROMATOGRAPHY; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BIOTECHNOLOGY; CELL LYSATE; FERMENTATION; FLOW RATE; MEMBRANE FILTER; POROSITY; PRECIPITATION; SCALE UP; SUPERNATANT;

ADSORPTION; AMMONIUM SULFATE; ANIMALS; BIOTECHNOLOGY; CATTLE; FRACTIONAL PRECIPITATION; IMMUNOGLOBULINS; SERUM; SERUM ALBUMIN, BOVINE; ULTRAFILTRATION;

BOVINAE;

EID: 56449122218     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21964     Document Type: Article

References (37)

1. Bacchin P, Aimar P, Field RW. 1996. Critical and sustainable fluxes: Theory, experiments and applications. J Memb Sci 281:42-69.
2. Baruah G, Nayak A, Winkelman E, Belfort G. 2004. Purification of monoclonal antibodies derived from transgenic goat milk by ultrafiltration. Biotechnol Bioeng 93:747-754.
3. Belfort G, Davis R, Zydney A. 1994. The behavior of suspensions and macromolecular solutions in crossflow microfiltration. J Memb Sci 96:1-58.
4. Bentham AC, Ireton MJ, Hoare M, Dunnill P. 1988. Protein precipitate recovery using microporous membranes. Biotechnol Bioeng 31:984-994.
5. Bramaud C, Aimar P, Daufin G. 1997. Whey protein fractionation: Isoelectric precipitation of a-lactalbumin under gentle heat treatment. Biotechnol Bioeng 56:391-397.
6. Chen V, Fane AG, Madaeni S, Wenten IG. 1997. Particle deposition during membrane filtration of colloids: Transition between concentration polarization and cake formation. J Memb Sci 125:109-122.
7. Devereux N, Hoare M. 1986. Membrane separation of protein precipitates: Studies with cross flow in hollow fibers. Biotechnol Bioeng 28:422-431.
8. Englard S, Seifter S. 1990. Precipitation techniques. Meth Immunol 182:285-290.
9. Field RW, Wu D, Howell JA, Gupta BB. 1995. Critical-flux concept for microfiltration fouling. J Memb Sci 100:259-272.
10. Garvery JS, Cremer NE, Sussdorf DH. 1979. Methods in immunology. 3rd edn. Reading, MA: Benjamin Inc. p 218-219.
11. Ge X, Trabbic Carlson K, Chilkoti A, Filipe C. 2006. Purification of an elastin-like fusion protein by microfiltration. Biotechnol Bioeng 95:424-432.
12. Ghosh R. 2004. Separation of human albumin and IgG by a membrane-based integrated bioseparation technique involving simultaneous precipitation, microfiltration and membrane adsorption. J Memb Sci 237:109-117.
13. Goheen S, Matson R. 1985. Purification of human serum gamma globulins by hydrophobic interaction high-performance liquid chromatography. J Chromotogr 326:235-241.
14. Higuchi A, Mishima S, Nakagawa T. 1991. Separation of proteins by surface modified polysulfone membranes. J Memb Sc 57:185-195.
15. Howell JA. 1995. Sub-critical flux operation of microfiltration. J Memb Sci 105:147-157.
16. Ito Y. 2000. Centrifugal precipitation chromatography: Principle, apparatus and optimization of key parameters for protein fractionation by ammonium sulfate precipitation. Anal Biochem 277:143-153.
17. Kluge T, Rezende C, Wood D, Belfort G. 1999. Protein transmission during dean vortex microfiltration of yeast suspensions. Biotechnol Bioeng 65:649-658.
18. Kurnik R, Yu A, Blank G, Burton A, Smith D, Athalye A, van Reis R. 1995. Buffer exchange using SEC, countercurrent dialysis, and tangential flow filtration: Models, development and industrial application. Biotechnol Bioeng 45:149-157.
19. Kwon DY, Vigneswaran S, Fane AG, Ben Aim R. 2000. Experimental determination of critical flux in corssflow microfiltration. Sep Purif Tech 19:169-181.
20. Lightfoot E. 2007. Ideal membrane cascades for downstream processing centrifugation during mammalian cell culture. 234th ACS National Meeting, Boston.
21. Maiorella B, Dorin G, Carion A, Harano D. 1991. Crossflow microfiltration of animal cells. Biotechnol Bioeng 37:121-126.
22. Manabe T, Takahashi Y, Okuyama T. 1987. Identification of fetal and adult serum/plasma proteins by two-dimensional electrophoresis and immunochemical staining. Electrophoresis 8:573-579.
23. Muller A, Chaufer B, Merin U, Daufin G. 2003. Purification of α-lactalbumin from a prepurified acid whey: Ultrafiltration or precipitation. Lait 83:439-451.
24. Nystrom M, Aimar P, Luque S, Kulovaara M, Metsamuuronen S. 1998. Fractionation of model proteins using their physiochemical properties. Coll Surf A: Physicochem Eng Aspects 138:185-205.
25. Ohno S, Koyama K, Fukuda M. 1981. US Patent 4,347,138.
26. Putnam F. 1975. Plasma proteins handbook. New York: Academic Press. 1st edn. p 58-125.
27. Saksena S, Zydney A. 1994. Effect of solution pH and ionic strength on the separation of albumin from immunoglobulins (IgG) by selective filtration. Biotechnol Bioeng 43:960-968.
28. Saksena S, Zydney AL. 1997. Influence of protein-protein interactions on bulk mass transport during ultrafiltration. J Memb Sci 125:93-108.
29. Scopes R. 1994. Protein purification - Principles and practice. 3rd edn. New York: Springer Verlag. p 76-85.
30. Sethuraman A, Han M, Kane RS, Belfort G. 2004. Effect of surface wettability of adhesion of proteins. Langmuir 20:7779-7788.
31. Taniguchi M, Belfort G. 2004. Low protein fouling synthetic membranes by UV-assisted surface grafting modification: Varying monomer type. J Memb Sci 231:147-157.
32. Tessier P, Sandler S, Lenhoff A. 2003. Self-interaction chromatography: A novel screening method for rational protein crystallization. Acta Cryst D 58:1531-1535.
33. van Reis R, Leonard L, Hsu C, Builder S. 1991. Industrial scale harvest of proteins from mammalian cell culture by tangential flow filtration. Biotechnol Bioeng 38:413-422.
34. van Reis R, Brake JM, Charkoudian J, Burns DB, Zydney AL. 1999. High performance tangential flow filtration using charged membranes. J Memb Sci 159:133-142.
35. Wang L, Sun X, Ghosh R. 2008. Purification of Equine IgG using membrane based enhanced hybrid bioseparation technique: A potential method for manufacturing hyperimmune antibody. Biotechnol Bioeng 99:625-633.
36. Zeman L, Zydney A. 1996. Microfiltration and ultrafiltration: Principles and applications. NY: Marcel Dekker. p 390-396.
37. Zhou JX, Tressel T. 2006. Basic concepts in Q membrane chromatography for large scale antibody production. Biot Prog 22:341-349.