Selective antibody precipitation using polyelectrolytes: A novel approach to the purification of monoclonal antibodies

Biotechnology and Bioengineering

Volumn 102, Issue 4, 2009, Pages 1141-1151

  McDonald, Paul ^a   Victa, Corazon ^a   Carter Franklin, Jayme N ^a   Fahrner, Robert ^a  

^a GENENTECH INC   (United States)

Author keywords

Monoclonal antibody; Polyelectrolytes; Precipitation; Purification

Indexed keywords

ANION-EXCHANGE CHROMATOGRAPHIES; ANIONIC POLYELECTROLYTES; ANTIBODY FRAGMENTS; BIOLOGICAL ACTIVITIES; CHARGE NEUTRALIZATIONS; HOST CELL PROTEINS; HOST CELLS; HUMANIZED MONOCLONAL ANTIBODIES; IONIC INTERACTIONS; POLISHING STEPS; POLY-ACRYLIC ACIDS; POLYVINYLSULFONIC ACIDS; PRECIPITATION; PROTEIN A; RESOLUBILIZATION; SOLUTION PH;

ALLERGIES; CARBOXYLIC ACIDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; IMPACT STRENGTH; IMPURITIES; LEACHING; MERGERS AND ACQUISITIONS; MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES; NUCLEIC ACIDS; ORGANIC ACIDS; POLYELECTROLYTES; POLYMERS; POLYSTYRENES; PURIFICATION; SURFACE PLASMON RESONANCE;

IONIC STRENGTH;

CELL PROTEIN; DNA; GENTAMICIN; INSULIN; MONOCLONAL ANTIBODY; POLYACRYLIC ACID; POLYELECTROLYTE; POLYSTYRENESULFONIC ACID; POLYVINYL DERIVATIVE; POLYVINYLSULFONIC ACID; PROTEIN A; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG; ACRYLIC ACID RESIN; CARBOPOL 940; LYAPOLATE; POLYSTYRENE DERIVATIVE;

ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; HOST CELL; IONIC STRENGTH; MOLECULAR WEIGHT; NONHUMAN; PH; PRECIPITATION; PURIFICATION; SOLUBILIZATION; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CHO CELL; CRICETULUS; HAMSTER; ISOLATION AND PURIFICATION; METABOLISM; OSMOLARITY; PROTEIN BINDING;

ACRYLIC RESINS; ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CRICETINAE; CRICETULUS; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; OSMOLAR CONCENTRATION; POLYSTYRENES; POLYVINYLS; PRECIPITATION; PROTEIN BINDING; SULFONIC ACIDS;

EID: 60549099544     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22127     Document Type: Article

References (28)

1. Carlsson F, Malmsten M, Linse P. 2003. Protein-polyelectrolyte cluster formation and redissolution: A Monte Carlo study. J Am Chem Soc 125:3140-3149.
2. Champion KM, Arnott D, Henzel WJ, Hermes S, Weikert S, Stults J, Vanderlaan M, Krummen L. 1999. A two-dimensional protein map of Chinese hamster ovary cells. Electrophoresis 20:994-1000.
3. Dainiak MB, Izumrudov VA, Muronetz VI, Galaev IY, Mattiasson B. 1999. Affinity precipitation of monoclonal antibodies by nonstoichiometric polyelectrolyte complexes. Bioseparation 7:231-240.
4. Dubin PL, Gao J, Mattison KW. 1994. Protein purification by selective phase separation with polyelectrolytes. Sep Purif Methods 23:1-16.
5. Fahrner RL, Knudsen HL, Basey CD, Galan W, Feuerhelm D, Vanderlaan M, Blank GS. 2001. Industrial purification of pharmaceutical antibodies: Development, operation, and validation of chromatography processes. Biotechnol Genet Eng Rev 18:302-327.
6. Ge X, Trabbbic-Carlson K, Chilkoti A, Filipe CDM. 2006. Purification of an elastin-like fusion protein by microfiltration. Biotechnol Bioeng 95:424-432.
7. Hallberg RK, Dubin PL. 1998. Effect of pH on the binding of (β-lactoglobulin to sodium polystyrenesulfonate. J Phys Chem B 102:8629-8633.
8. Hattori T, Hallberg R, Dubin PL. 2000. Roles of electrostatic interaction and polymer structure in the binding of (β-lactoglobulin to anionic poly-electrolytes: Measurement of binding constants by frontal analysis continuous capillary electrophoresis. Langmuir 16:9738-9743.
9. Hilbrig F, Freitag R. 2003. Protein purification by affinity precipitation. J Chromatogr B 790:79-90.
10. Kaibara K, Okazaki T, Bohidar HB, Dubin PL. 2000. pH-induced coacervation in complexes of bovine serum albumin and cationic polyelectrolytes. Biomacromolecules 1:100-107.
11. Kelley B, Tobler SA, Brown P, Coffman J, Godavarti R, Iskra T, Switzer M, Vunnum S. 2008. Weak partitioning chromatography for anion exchange purification of monoclonal antibodies. Biotechnol Bioeng 101:553-566.
12. Kim JY, Mulchandani A, Chen W. 2005. Temperature-triggered purification of antibodies. Biotechnol Bioeng 90:373-379.
13. Kumar A, Wahlund PO, Kepka C, Galaev IY. 2003. Purification of histidine-tagged single-chain Fv-antibody fragments by metal chelate affinity precipitation using thermoresponsive copolymers. Biotechnol Bioeng 84:494-503.
14. Low D, O'Leary R, Pujar RN. 2007. Future of antibody purification. J Chromatogr B 848:48-63.
15. Mattiasson B, Dainyak MB, Galaev IY. 1998a. Smart polymers and protein purification. Polym-Plast Technol Eng 37:303-308.
16. Mattiasson B, Kumar A, Galaev IY. 1998b. Affinity precipitation of proteins: Design criteria for an efficient polymer. J Mol Recognit 11:211-216.
17. Mattison KW, Dubin PL, Brittain IJ. 1998. Complex formation between bovine serum albumin and strong polyelectrolytes: Effect of polymer charge density. J Phys Chem B 102:3830-3836.
18. Milletti F, Storchi L, Sforna G, Cruciani G. 2007. New and original pKa prediction method using grid molecular interaction fields. J Chem Inf Mod 47:2172-2181.
19. Powers DD, Carbonell RG, Kilpatrick PK. 1994. Affinity precipitation of an antibody by ligand-modified phospholipids. Biotechnol Bioeng 44:509-522.
20. Shukla AA, Hubbard B, Tressel T, Guhan S, Low D. 2007. Downstream processing of monoclonal antibodies-application of platform approaches. J Chromatogr B 848:28-39.
21. Smith BD, Soellner MB, Raines RT. 2003. Potent inhibition of ribonuclease A by oligo(vinylsulfonic acid). J Biol Chem 278:20934-20938.
22. Taipa MA, Kaul R, Mattiasson B, Cabral JMS. 1998. Preliminary studies on the purification of a monoclonal antibody by affinity precipitation with Eudragit S-100. J Mol Recognit 11:240-242.
23. Taipa MA, Kaul RH, Mattiasson B, Cabral JMS. 2001. Recovery of a monoclonal antibody from hybridoma culture supernatant by affinity precipitation with Eudragit S-100. Bioseparation 9:291-298.
24. Thommes J, Etzel M. 2007. Alternatives to chromatography separations. Biotechnol Prog 23:42-45.
25. Venkiteshwaran A, Heider P, Teysseyre L, Belfort G. 2008. Selective precipitation-assisted recovery of immunoglobulins from bovine serum using controlled-fouling crossflow membrane microfiltration. Biotechnol Bioeng 101:957-966.
26. Wang Y, Gao JY, Dubin PL. 1996. Protein separation via polyelectrolyte coacervation: Selectivity and efficiency. Biotechnol Prog 12:356-362.
27. Wurm FM. 2004. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:1393-1398.
28. Xia J, Dubin PL. 1994. Protein-polyelectrolyte complexes. In: Dubin PL, Davis RM, Bock J, Schulz D, Thies C, editors. Macromolecular complexes in chemistry and biology. Springer Verlag. Berlin: p 247-271.