Phylogenetic relationships and morphological diversity in Neotropical Heliotropium (Heliotropiaceae)

Taxon

Volumn 60, Issue 3, 2011, Pages 663-680

  Luebert, Federico ^a,b   Brokamp, Grischa ^a   Wen, Jun ^c   Weigend, Maximilian ^a   Hilger, Hartmut H ^a  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b UNIVERSITY OF CHILE   (Chile)

^c DEPARTMENT OF PALEOBIOLOGY   (United States)

Author keywords

Andes; Boraginaceae; Boraginales; Heliotropiaceae; Heliotropium; Morphological diversity

Indexed keywords

ADAPTIVE RADIATION; HUMID ENVIRONMENT; LEAF MORPHOLOGY; NEOTROPICAL REGION; PHYLOGENETICS; PHYLOGENY; PLASTID; SPECIES DIVERSITY;

ANDES; ATACAMA DESERT; CHILE;

BORAGINACEAE; HELIOTROPIUM; TOURNEFORTIA;

EID: 79959710422     PISSN: 00400262     EISSN: None     Source Type: Journal    
DOI: 10.1002/tax.603004     Document Type: Article

References (142)

1. Ackermann, M. & Weigend, M. 2006. Nectar, floral morphology and pollination syndrome in Loasaceae subfam. Loasoideae (Cornales). Ann. Bot. (Oxford) 98: 503-514.
2. Adams, D.C., Berns, C.M., Kozak, K.H. & Wiens, J.J. 2009. Are rates of species diversification correlated with rates of morphological evolution? Proc. Roy. Soc. London, Ser. B, Biol. Sci. 276: 2729-2738.
3. Akhani, H. & Förther, H. 1994. The genus Heliotropium L. (Boraginaceae) in Flora Iranica area. Sendtnera 2: 187-276.
4. Álvarez, I. & Wendel, J.F. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Molec. Phylog. Evol. 29: 417-434.
5. Anderson, M.J. 2006. Distance-based tests for homogeneity of multivariate dispersions. Biometrics 62: 245-253.
6. Ariza-Espinar, L. 2006. 252. Boraginaceae, parte 1 (excepto Heliotropium). Pp. 3-55 in: Ariza-Espinar, L., Calviño, A., di Fulvio, T.E. & Dottori, N. (eds.), Flora fanerogámica Argentina, fasc. 97. Córdoba: Proflora CONICET. *
7. Baker, J.G. & Wright, C.H. 1906. Boragineae. Pp. 5-62 in: Thiselton-Dyer, W.T. (ed.), Flora of tropical Africa, vol. 4, sect. 2. London: Reeve. *
8. Baker, R.H. & DeSalle, R. 1997. Multiple sources of character information and the phylogeny of Hawaiian Drosophilids. Syst. Biol. 46: 654-673.
9. Blomberg, S.P., Garland, T. & Ives, A.R. 2003. Testing for phylogenetic signal in comparative data: Behavioral traits are more labile. Evolution 57: 717-745.
10. Boissier, P.E. 1879. Flora orientalis, vol. 4, Corolliflorae et Monochlamydeae. Geneva, Basel: G. Bibliopolam. *
11. Bremer, K. 1988. The limits of amino-acid sequence data in angiosperm phylogenetic reconstruction. Evolution 42: 795-803.
12. Brummitt, R.K. 1972. Heliotropium L. Pp. 84-86 in: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M. & Webb, D.S. (eds.), Flora europaea, vol. 3. Cambridge: Cambridge University Press. *
13. Cailliez, F. 1983. The analytical solution of the additive constant problem. Psychometrika 48: 305-308.
14. Candolle, A.P. de. 1845. Prodromus systematis naturalis regni vegetabilis, vol. 9. Paris: Treuttel & Würtz.
15. Collar, D.C., Near, T.J. & Wainwright, P.C. 2005. Comparative analysis of morphological diversity: Does disparity accumulate at the same rate in two lineages of centrarchid fishes? Evolution 59: 1783-1794.
16. Collar, D.C., O'Meara, B.C., Wainwright, P.C. & Near, T.J. 2009. Piscivory limits diversification of feeding morphology in Centrarchid Fishes. Evolution 63: 1557-1573.
17. Correa, M.N. 1999. Boraginaceae. Pp. 116-145 in: Correa, M.N. (ed.), Flora patagónica, parte 6. Buenos Aires: Colección Científica del INTA. *
18. Correll, D.S. & Johnston, M.C. 1970. Manual of the vascular plants of Texas. Renner: Texas Research Foundation. *
19. Craven, L.A. 1996. A taxonomic revision of Heliotropium (Boraginaceae) in Australia. Austral. Syst. Bot. 9: 521-657.
20. Craven, L.A. 2005. Malesian and Australian Tournefortia transferred to Heliotropium and notes on delimitation of Boraginaceae. Blumea 50: 375-381.
21. Dawson, G. 1965. Boraginaceae. Pp. 100-121 in: Cabrera, A.L. (ed.), Flora de la Provincia de Buenos Aires. Buenos Aires: Colección Científica del INTA. *
22. Di Fulvio, T.E. 1969. Recuentos cromosómicos en Heliotropium (Boraginaceae). Kurtziana 5: 89-94.
23. Di Fulvio, T.E. 1978. Sobre la vasculatura floral, embriología y chromosomas de Ixorhea tschudiana (Heliotropiaceae). Kurtziana 11: 75-105.
24. Di Fulvio, T.E. 1982. Sobra la anatomía foliar y estomatogénesis de Ixorhea tschudiana (Heliotropiaceae). Kurtziana 15: 9-18.
25. Di Fulvio, T.E. 2003. Heliotropium abbreviatum (Boraginaceae), su hallazgo en Argentina. Kurtziana 30: 57-60.
26. Di Fulvio, T.E. & Ariza-Espinar, L. 2004. Novedades taxonómicas y nomenclaturales en Heliotropium (Boraginaceae) de América del Sur. Bol. Soc. Argent. Bot. 39: 115-124.
27. Diane, N., Förther, H. & Hilger, H.H. 2002. A systematic analysis of Heliotropium, Tournefortia, and allied taxa of the Heliotropiaceae (Boraginales) based on ITS1 sequences and morphological data. Amer. J. Bot. 89: 287-295.
28. Diane, N., Jacob, C. & Hilger, H.H. 2003. Leaf anatomy and foliar trichomes in Heliotropiaceae and their systematic relevance. Flora 198: 468-485.
29. Doyle, J.J. & Doyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15.
30. Dray, S., Dufour, A.B. & Chessel, D. 2007. The ade4 package-II: Two-table and K-table methods. R News 7: 47-52.
31. Edwards, S.V. 2009. Is a new and general theory of molecular systematics emerging? Evolution 63: 1-19.
32. Farris, J.S., Källersjö, M., Kluge, A.G. & Bult, C. 1994. Testing significance of incongruence. Cladistics 10: 315-319.
33. Farris, J.S., Kluge, A.G. & Eckardt, M.J. 1970. A numerical approach to phylogenetic systematics. Syst. Zool. 19: 172-191.
34. Feinbrun-Dothan, N. 1978. Flora Palestina, part 3, Ericaceae to Compositae. Jerusalem: The Israel Academy of Sciences and Humanities. *
35. Felsenstein, J. 1978. Cases in which parsimony or compatibility methods will be positively misleading. Syst. Zool. 27: 401-410.
36. Felsenstein, J. 1981. Evolutionary trees from DNA sequences: A maximum likelihood approach. J. Molec. Evol. 17: 368-376.
37. Felsenstein, J. 1985. Phylogenies and the comparative method. Amer. Naturalist 125: 1-15.
38. Felsenstein, J. 1988. Phylogenies and quantitative characters. Annual Rev. Ecol. Syst. 19: 445-471.
39. Ferguson, D.M. 1999. Phylogenetic analysis and relationships in Hydrophyllaceae based on ndhF sequence data. Syst. Bot. 23: 253-268.
40. Feuillet, C. 2008. Folia Taxonomica 4. Conspectus of Myriopus (Heliotropiaceae: Boraginales) in the Guiana Shield. J. Bot. Res. Inst. Texas 2: 263-265.
41. Foote, M. 1993. Contributions of individual taxa to overall morphological disparity. Paleobiology 19: 403-419.
42. Foote, M. 1997. The evolution of morphological diversity. Annual Rev. Ecol. Syst. 28: 129-152.
43. Foote, M. 1999. Morphological diversity in the evolutionary radiation of Paleozoic and post-Paleozoic crinoids. Paleobiology 25: 1-116.
44. Förther, H. 1998. Die infragenerische Gliederung der Gattung Heliotropium L. und ihre Stellung innerhalb der subfam. Heliotropioideae (Schrad.) Arn. (Boraginaceae). Sendtnera 5: 35-241.
45. Frohlich, M.W. 1978. Systematics of Heliotropium section Orthostachys in Mexico. Ph.D. Dissertation, Harvard University, Cambridge, Massachusetts, U.S.A.
46. Frohlich, M.W. 1981. Heliotropium. Pp. 70-104 in: Gómez-Pompa, A. (ed), Flora de Veracruz, vol. 18. Xalapa: Instituto Nacional de Investigaciones sobre Recursos Bióticos. *
47. Gangui, N. 1955. Las especies silvestres de Heliotropium de la República Argentina. Revista Fac. Ci. Exact. 17: 481-560.
48. Garland, T. 1992. Rate tests for phenotypic evolution using phylogenetically independent contrasts. Amer. Naturalist 140: 509-519.
49. Gibson, D.N. 1970. Boraginaceae in Flora of Guatemala-part IX, numbers 1 and 2. Fieldiana, Bot. 24: 111-167.
50. Gottschling, M., Diane, N., Hilger, H.H. & Weigend, M. 2004. Testing hypotheses on disjunctions present in the primarily woody Boraginales: Ehretiaceae, Cordiaceae, and Heliotropiaceae, inferred from ITS1 sequence data. Int. J. Pl. Sci. 165: S123-S135.
51. Gottschling, M., Hilger, H.H., Wolf, M. & Diane, N. 2001. Secondary structure of the ITS1 transcript and its application in a reconstruction of the phylogeny of Boraginales. Pl. Biol. 3: 629-636.
52. Gower, J.C. 1971. A general coefficient of similarity and some of its properties. Biometrics 27: 857-871.
53. Gower, J.C. & Legendre, P. 1986. Metric and Euclidean properties of dissimilarity coefficients. J. Classific. 3: 5-48.
54. Graham, A. & Jarzen, D.M. 1969. Studies in Neotropical paleobotany. I. The Oligocene communities of Puerto Rico. Ann. Missouri Bot. Gard. 56: 308-357.
55. Gürke, M. 1893. Boraginaceae. Pp. 59-96 in: Engler, A. & Prantl, K. (eds), Die natürlichen Pflanzenfamilien, vol. 4. Leipzig: Engelmann.
56. Hamilton, M.B. 1999. Four primer pairs for the amplification of chloroplast intergenic regions with intraspecific variation. Molec. Ecol. 8: 521-523.
57. Harmon, L.J., Weir, J.T., Brock, C.D., Glor, R.E. & Challenger, W. 2008. GEIGER: Investigating evolutionary radiations. Bioinformatics 24: 129-131.
58. Hilger, H.H. 1992. Morphology of Heliotropium (Boraginaceae) dispersal units. Bot. Acta 105: 387-393.
59. Hilger, H.H. & Diane, N. 2003. A systematic analysis of Heliotropiaceae (Boraginales) based on trnL and ITS1 sequence data. Bot. Jahrb. Syst. 125: 19-51.
60. Hoogeveen, J. 2009. Global map of aridity-10 arc minutes. Rome: FAO.
61. Huelsenbeck, J.P. 1997. Is the Felsenstein zone a fly trap? Syst. Biol. 46: 69-74.
62. Hughes, C. & Eastwood, R. 2006. Island radiation on a continental scale: Exceptional rates of plant diversification after uplift of the Andes. Proc. Natl. Acad. Sci. U.S.A. 103: 10334-10339.
63. Hutcheon, J.M. & Garland, T., Jr. 2004. Are megabats big? J. Mammal. Evol. 11: 257-277.
64. Jabaily, R.S. & Sytsma, K.J. 2010. Phylogenetics of Puya (Bromeliaceae): Placement, major lineages, and evolution of Chilean species. Amer. J. Bot. 97: 337-356.
65. Jaramillo, M.A. & Manos, P.S. 2001. Phylogeny and patterns of floral diversity in the genus Piper (Piperaceae). Amer. J. Bot. 88: 706-716.
66. Johnston, I.M. 1928. Studies in the Boraginaceae VII. The South American species of Heliotropium. Contr. Gray Herb. 81: 3-73.
67. Johnston, I.M. 1929. Papers on the flora of northern Chile. Contr. Gray Herb. 85: 1-163.
68. Johnston, I.M. 1930. Studies in the Boraginaceae VIII. I. Observations on the species of Cordia and Tournefortia known from Brazil. 3. Treatment of Tournefortia. Contr. Gray Herb. 92: 66-89.
69. Johnston, I.M. 1935a. Studies in the Boraginaceae X. The Boraginaceae of Northeastern South America. J. Arnold Arbor. 16: 1-64.
70. Johnston, I.M. 1935b. Studies in the Boraginaceae XI. 1. The species of Tournefortia and Messerschmidia in the Old World. J. Arnold Arbor. 16: 145-168.
71. Johnston, I.M. 1937. Studies in the Boraginaceae XII. 2. Novelties and critical notes. J. Arnold Arbor. 18: 10-25.
72. Johnston, I.M. 1964. Boraginaceae. Pp. 123-221 in: Lundell, C.L. (ed.), Flora of Texas, vol. 1. Renner: Texas Research Foundation.
73. Katoh, K., Misawa, K., Kuma, K. & Miyata, T. 2002. MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucl. Acids Res. 30: 3059-3066.
74. Kerkhoff, A.J. & Enquist, B.J. 2009. Multiplicative by nature: Why logarithmic transformation is necessary in allometry. J. Theor. Biol. 257: 519-521.
75. Killip, E.P. Unpub. manuscript, ca. 1938. The Andean species of Tournefortia. Washington, D.C.: Smithsonian Institution Archives. *
76. Kim, S.-T. & Donoghue, M.J. 2008. Incongruence between cpDNA and nrITS trees indicates extensive hybridization within Eupersicaria (Polygonaceae). Amer. J. Bot. 95: 1122-1135.
77. Klotzsch, F. 1852. Heliotropium submolle, eine neue Art aus dem Freistaate Ecuador. Allg. Gartenzeitung 20: 89-91.
78. Kunkel, G. 1974. Flora de Gran Canaria, tomo 1. Las Palmas: Ediciones del Excmo. *
79. Kunkel, G. 1977. Endemismos canarios. Inventario de las plantas vasculares endémicas de la Provincia de Las Palmas. Madrid: Instituto Nacional para la Conservación de la Naturaleza. *
80. Lambkin, C.L., Lee, M.S.Y., Winterton, S.L. & Yeates, D.K. 2002. Partitioned Bremer support and multiple trees. Cladistics 18: 436-444.
81. Luebert, F. & Wen, J. 2008. Phylogenetic analysis and evolutionary diversification of Heliotropium sect. Cochranea (Heliotropiaceae) in the Atacama Desert. Syst. Bot. 33: 390-402.
82. Luque, T. 1996. Karyological studies on Spanish Boraginaceae. VII. Heliotropium L. Willdenowia 25: 659-668.
83. Macbride, J.F. 1916. Certain Boraginaceae, new or transferred. Proc. Amer. Acad. Arts 51: 541-548.
84. Macbride, J.F. 1960. Flora of Peru: Boraginaceae. Publ. Field. Mus. Nat. Hist., Bot. Ser. 13: 539-609.
85. Maddison, W.P. 1997. Gene trees in species trees. Syst. Biol. 46: 523-536.
86. Maechler, M., Rousseeuw, P., Struyf, A. & Hubert, M. 2005. Cluster analysis basics and extensions. http://cran.r-project.org/web/pack ages/cluster/ (accessed 14 April 2010).
87. Martins, E.S. 1990. 116. Boraginaceae. Pp. 59-114 in: Launert, E. & Pope, G.V. (eds.), Flora zambesiaca, vol. 7, part 4. London: Flora Zambesiaca Managing Committee. *
88. Martorell, C. & Ezcurra, E. 2007. The narrow-leaf syndrome: A functional and evolutionary approach to the form of fog-harvesting rosette plants. Oecologia 151: 561-573.
89. Mau, B., Newton, M.A. & Larget, B. 1999. Bayesian phylogenetic inference via Markov chain Monte Carlo methods. Biometrics 55: 1-12.
90. Melo, J.I.M. & Semir, J. 2006. Euploca rodaliae J.I.M. M elo & Semir-a new species of Euploca (Heliotropiaceae) from Brazil. Candollea 61: 453-456.
91. Melo, J.I.M. & Semir, J. 2008. Taxonomia do gênero Heliotropium L. (Heliotropiaceae) no Brasil. Acta Bot. Brasil. 22: 754-770.
92. Melo, J.I.M. & Semir, J. 2009. Two new Brazilian species and new combinations in Euploca (Heliotropiaceae). Kew Bull. 64: 285-289.
93. Melo, J.I.M. & Semir, J. 2010. Taxonomia do gênero Euploca Nutt. (Heliotropiaceae) no Brasil. Acta Bot. Brasil. 24: 111-132.
94. Middleton, N. & Thomas, D.S.G. 1992. World atlas of desertification: United Nations Environment Programme. London: Arnold.
95. Miers, J. 1868. Observations on some of the Heliotropieae. Ann. Mag. Nat. Hist., ser. 4, 4: 121-133.
96. Miller, J.S. 1988. A revised treatment of Boraginaceae for Panama. Ann. Missouri Bot. Gard. 75: 456-521.
97. Miller, J.S. 2001. Boraginaceae. Pp. 435-455 in: Stevens, W.D., Ulloa Ulloa, C., Pool, A. & Montiel, M. (eds.), Flora de Nicaragua, tomo 1. St. Louis, Missouri: Missouri Botanical Garden. *
98. Mooers, A. Ø. & Heard, S.B. 1997. Inferring evolutionary process from the phylogenetic tree shape. Quart. Rev. Biol. 72: 31-54.
99. Moore, M.J. & Jansen, R.K. 2006. Molecular evidence for the age, origin, and evolutionary history of the American desert plant genus Tiquilia (Boraginaceae). Molec. Phylog. Evol. 39: 668-687.
100. Munz, P. & Keck, D. 1965. A California flora. Berkeley: University of California Press. *
101. Nowicke, J.W. 1969. Flora of Panama, part IX, family 167. Boraginaceae. Ann. Missouri Bot. Gard. 56: 33-69.
102. Nowicke, J.W. & Miller, J.S. 1991. Boraginaceae. Pp. 3-33 in: Dassanayake, M.D. (ed.), A revised handbook of the flora of Ceylon. Washington, D.C.: Smithsonian Institution. *
103. O'Meara, B.C., Ane, C., Sanderson, M.J. & Wainwright, P.C. 2006. Testing for different rates of continuous trait evolution using likelihood. Evolution 60: 922-933.
104. Oksanen, J., Blanchet, F.G., Kindt, R., Legendre, P., O'Hara, R.G., Simpson, G.L., Solymos, P., Stevens, M.H.H. & Wagner, H. 2010. vegan: Community Ecology Package. R package version 1.17-0. http://CRAN.R-project.org/package=vegan/ (accessed 14 April 2010).
105. Pathan, A.K., Bond, J. & Gaskin, R.E. 2008. Sample preparation for scanning electron microscopy of plant surfaces-Horses for courses. Micron 39: 1049-1061.
106. Pérez-Moreau, R.L. 1963. Una nueva especie de Heliotropium de Neuquén (Boraginaceae). Darwiniana 12: 629-632.
107. Pérez-Moreau, R.L. 1994. Boraginaceae. Pp. 3-35 in: Pérez-Moreau, R.L. (ed.), Flora Chaqueña, vol. 8. Buenos Aires: Instituto Nacional de Tecnología Agropecuaria. *
108. Pérez-Moreau, R.L. & Cabrera, A.L. 1983. Boraginaceae. Pp. 247-291 in: Cabrera, A.L. (ed.), Flora de la provincia de Jujuy, República Argentina, vol. 8. Buenos Aires: Colección Científica del INTA. *
109. Perret, M., Chautems, A., Spichiger, R., Barraclough, T.G. & Savolainen, V. 2007. The geographical pattern of speciation and floral diversification in the Neotropics: The tribe Sinningieae (Gesneriaceae) as a case study. Evolution 61: 1641-1660.
110. Posada, D. & Crandall, K.A. 1998. MODELTEST: Testing the model of DNA substitution. Bioinformatics 14: 817-818.
111. Purvis, A. 2004. Evolution: How do characters evolve? Nature 432: 166.
112. R Development Core Team. 2009. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing.
113. Rambaut, A. 1996. Se-Al: Sequence Alignment Editor. http://tree.bio.ed.ac.uk/software/seal/ (accessed 12 December 2009).
114. Reiche, K. 1907. Estudios críticos sobre la flora de Chile. Boraginaceae. Anales Univ. Chile 121: 227-282.
115. Richardson, A. & King, W.K. 2009. Tournefortia hirsutissima (Boraginaceae) new to the flora of Texas. J. Bot. Res. Inst. Texas 3: 465-467.
116. Ricklefs, R.E. 2004. Cladogenesis and morphological diversification in passerine birds. Nature 430: 338-341.
117. Ricklefs, R.E. 2006. Time, species, and the generation of trait variance in clades. Syst. Biol. 55: 151-159.
118. Riedl, H. 1997. Boraginaceae. Pp. 43-144 in: Kalkman, C., Kirkup, D.W., Nooteboom, H.P., Stevens, P.F. & de Wilde, W.J.J.O. (eds.), Flora Malesiana, Series I-Seed Plants, vol. 13. Leiden: Rijksherbarium. *
119. Riedl, H. & Edwards, S. 2006. 157. Boraginaceae. Pp. 64-102 in: Hedberg, I., Kelbessa, E., Edwards, S., Demisew, S. & Persson, E. (eds.), Flora of Ethiopia and Eritrea, vol. 5. Addis Ababa: National Herbarium, Addis Ababa University; Uppsala: Dept. of Syst. Bot., Uppsala University. *
120. Ronquist, F. & Huelsenbeck, J.P. 2003. MrBayes 3: Bayesian phylogenetic inference u nder mixed models. Bioinformatics 19: 1572-1574.
121. Rusby, H.H. 1895. On the collections of Mr. Miguel Bang in Bolivia, part II. Mem. Torrey Bot. Club 4: 203-274.
122. Schliep, K. 2010. phangorn: Phylogenetic analysis in R. http://CRAN.R-project.org/package=phangorn (accessed 18 November 2010).
123. Schluter, D. 2000. The ecology of adaptive radiation. Oxford: Oxford University Press.
124. Schönfelder, P. & Schönfelder, I. 1997. Die Kosmos-Kanarenflora. Stuttgart: Franckh-Kosmos. *
125. Shaw, J., Lickey, E.B., Beck, J.T., Farmer, S.B., Liu, W., Miller, J., Siripun, K.C., Winder, C.T., Schilling, E.E. & Small, R.L. 2005. The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Amer. J. Bot. 92: 142-166.
126. Shimodaira, H. & Hasegawa, M. 1999. Multiple comparisons of loglikelihoods with applications to phylogenetic inference. Molec. Biol. Evol. 16: 1114-1116.
127. Sidlauskas, B. 2007. Testing for unequal rates of morphological diversification in the absence of a detailed phylogeny: A case study from characiform fishes. Evolution 61: 299-316.
128. Slowinski, J. & Page, R.D.M. 1999. How should species phylogenies be inferred from sequence data? Syst. Biol. 48: 814-825.
129. Smith, S.A. & Donoghue, M.J. 2008. Rates of molecular evolution are linked to life history in flowering plants. Science 322: 86-89.
130. Smith, S.D. & Baum, D.A. 2006. Phylogenetics of the florally diverse Andean clade Iochrominae (Solanaceae). Amer. J. Bot. 93: 1140-1153.
131. Sorenson, M.D. & Franzosa, E.A. 2007. TreeRot. Version 3. Boston: Boston University.
132. Spegazzini, C.L. 1902. Nova addenda ad floram Patagonicam II. Anales Soc. Ci. Argent. 53: 66-80.
133. Struwe, L., Albert, V.A., Calio, M.F., Frasier, C., Lepis, K.B., Mathews, K.G. & Grant, J.R. 2009. Evolutionary patterns in Neotropical Helieae (Gentianaceae): Evidence from morphology, chloroplast and nuclear DNA sequences. Taxon 58: 479-499.
134. Swofford, D.L. 2003. PAUP*: Phylogenetic analysis using parsimony (*and other methods). Version 4.0 beta 10. Sunderland, Massachusetts: Sinauer.
135. Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Pl. Molec. Biol. 17: 1105-1109.
136. Taton, A. 1971. Boraginaceae. Pp. 1-80 in: Bamps, P. (ed.), Flore du Congo du Rwanda et du Burundi. Brussels: Jardin Botanique National de Belgique. *
137. Verdcourt, B. 1991. Boraginaceae. Pp. 1-124 in: Polhill, R.M. (ed.), Flora of tropical East Africa, vol. 152. Kew: Royal Botanic Gardens. *
138. Von Hagen, K.B. & Kadereit, J.W. 2001. The phylogeny of Gentianella (Gentianaceae) and its colonization of the southern hemisphere as revealed by nuclear and chloroplast DNA sequence variation. Org. Divers. Evol. 1: 61-79.
139. Wagner, P.J. 1997. Patterns of morphologic diversification among the Rostroconchia. Paleobiology 23: 115-150.
140. Weigend, M., Brokamp, G., Kirbach, A. & Förther, H. 2003. Notas sobre Heliotropium krauseanum Fedde, la ú nica especie de Heliotropium sect. Cochranea del Perú (Heliotropiaceae = Boraginaceae subfam. Heliotropioideae). Arnaldoa 10: 61-74.
141. Weigend, M. & Gottschling, M. 2006. Evolution of funnel-revolver flowers and ornithophily in Nasa (Loasaceae). Pl. Biol. 8: 120-142.
142. Zwickl, D.J. 2006. Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. Dissertation, University of Texas at Austin, Texas, U.S.A.