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Methods in Molecular Biology
Volumn 634, Issue , 2010, Pages 271-283
Fine-tuning enzyme activity through saturation mutagenesis

(1)  Hogrefe, Holly H a  

a NONE
Author keywords

Degenerate codon; Degenerate primer; Quik Change Multi; Saturation mutagenesis
Indexed keywords

ENZYME;
EID: 79958023522     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60761-652-8_20     Document Type: Article
Times cited : (4)
References (7)
  • 1
    • 0033280672 scopus 로고    scopus 로고
    • Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
    • Miyazaki K, Arnold FH (1999) Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function. J Mol Evol 49: 716-720
    • (1999) J Mol Evol , vol.49 , pp. 716-720
    • Miyazaki, K.1    Arnold, F.H.2
  • 2
    • 0036387195 scopus 로고    scopus 로고
    • Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant
    • Arezi B, Hansen CJ, Hogrefe HH (2002) Efficient and high fidelity incorporation of dye-terminators by a novel archaeal DNA polymerase mutant. J Mol Biol 322: 719-729
    • (2002) J Mol Biol , vol.322 , pp. 719-729
    • Arezi, B.1    Hansen, C.J.2    Hogrefe, H.H.3
  • 3
    • 0036841628 scopus 로고    scopus 로고
    • Creating randomized amino acid libraries with the QuikChange Multi site-directed mutagenesis kit
    • Hogrefe HH, Cline J, Youngblood GL, Allen RM (2002) Creating randomized amino acid libraries with the QuikChange Multi site-directed mutagenesis kit. Biotechniques 33: 1158-1165
    • (2002) Biotechniques , vol.33 , pp. 1158-1165
    • Hogrefe, H.H.1    Cline, J.2    Youngblood, G.L.3    Allen, R.M.4
  • 4
    • 84859256582 scopus 로고    scopus 로고
    • Generate multiple mutations quickly and efficiently
    • Newsletter S (2001) Generate multiple mutations quickly and efficiently. Strategies 14 (3): 74-75
    • (2001) Strategies , vol.14 , Issue.3 , pp. 74-75
    • Newsletter, S.1
  • 5
    • 84859259427 scopus 로고    scopus 로고
    • Site-directed mutagenesis by polymerase chain reaction
    • Braman J (ed), 2nd edn, Methods in molecular biology. Humana, Totowa, NJ
    • Jeltsch A, Lanio T, Jeltsch A, Lanio T (2002) Site-directed mutagenesis by polymerase chain reaction. In: Braman J (ed) In vitro mutagenesis protocols, 2nd edn, Methods in molecular biology. Humana, Totowa, NJ
    • (2002) Vitro Mutagenesis Protocols
    • Jeltsch, A.1    Lanio, T.2    Jeltsch, A.3    Lanio, T.4
  • 7
    • 34748889007 scopus 로고    scopus 로고
    • Efficient sitedirected saturation mutagenesis using degenerate oligonucleotides
    • Steffens DL, Williams JGK (2007) Efficient sitedirected saturation mutagenesis using degenerate oligonucleotides. J Biomol Tech 18: 147-149
    • (2007) J Biomol Tech , vol.18 , pp. 147-149
    • Steffens, D.L.1    Jgk, W.2

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.