Generation of eight adjacent mutations in a single step using a site-directed mutagenesis kit

Clinical Chemistry and Laboratory Medicine

Volumn 42, Issue 4, 2004, Pages 384-386

  Cabré, Anna ^a   Girona, Josefa ^a   Zalba, Guillermo ^b   Moreno, María Ujué ^b   Díez, Javier ^b   Masana, Lluís ^a  

^a UNIVERSITAT ROVIRA I VIRGILI   (Spain)

^b UNIVERSITY OF NAVARRA   (Spain)

Author keywords

Oligonucleotides; PCR; Regulatory elements; Site directed mutagenesis

Indexed keywords

PRIMER DNA;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; EXON; GENE DELETION; GENE INSERTION; GENE TARGETING; INTRON; METHODOLOGY; MUTATIONAL ANALYSIS; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION;

DNA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION;

EID: 2342478578     PISSN: 14346621     EISSN: None     Source Type: Journal    
DOI: 10.1515/CCLM.2004.068     Document Type: Article

References (3)

1. Hames BD, Higgins SJ. Gene transcription: a practical appproach. New York: Oxford University Press Inc., 1993.
2. Makarova O, Kamberov E, Margolis B. Generation of deletion and point mutations with one primer in a single cloning step. BioTechniques 2000;29:970-2.
3. Steer JH, Kroeger KM, Abraham LJ, Joyce DA. Glucorticoids suppress tumor necrosis factor-a expression by human monocytic THP-1 cells by suppressing transactivation through adjacent NF-κB and c-jun-activating transcription factor-2 binding sites in the promoter. J Biol Chem 2000;275:18432-40.