Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions, using QuikChange site-directed mutagenesis.

Methods in molecular biology (Clifton, N.J.)

Volumn 182, Issue , 2002, Pages 37-43

  Wang, Wenyan ^a   Malcolm, Bruce A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; METHODOLOGY; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; SYNTHESIS;

BASE SEQUENCE; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; GENETIC ENGINEERING; INDICATORS AND REAGENTS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTAGENESIS, INSERTIONAL; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; SEQUENCE DELETION;

EID: 0036134273     PISSN: 10643745     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (0)