Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase

BioTechniques

Volumn 31, Issue 1, 2001, Pages 88-92

  Geiser, M ^a   Cebe R ^a   Drewello, D ^a   Schmitz, R ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; DNA; ENZYMES; MUTAGENESIS;

AMPLIFIED FRAGMENTS;

BIOTECHNOLOGY;

DNA FRAGMENT; NUCLEOTIDE; PLASMID DNA; PLASMID VECTOR; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; RESTRICTION ENDONUCLEASE;

ARTICLE; DNA INTEGRATION; DNA SEQUENCE; GENE INSERTION; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TECHNIQUE;

EID: 0034947940     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01311st05     Document Type: Article

References (3)

1. Enzyme-free cloning: A rapid method to clone PCR products independent of vector restriction enzyme sites
2. DNA cloning without restriction enzyme and ligase
3. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange™ site-directed mutagenesis