Cleavage-resistant fusion proteins of the M2 muscarinic receptor and Gαi1. Homotropic and heterotropic effects in the binding of ligands

Biochimica et Biophysica Acta - General Subjects

Volumn 1810, Issue 6, 2011, Pages 592-602

  Ma, Amy W S ^a   Dong, John Y ^a   Ma, Dengbo ^a   Wells, James W ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Cooperativity; Fusion protein; G protein coupled receptor; G subunit; Oligomers; Radioligand binding studies

Indexed keywords

CHOLIC ACID; DIGITONIN; GUANINE NUCLEOTIDE; GUANYLYLIMIDODIPHOSPHATE; HYBRID PROTEIN; METHYLSCOPOLAMINE; MUSCARINIC M2 RECEPTOR INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA 1 FUSION PROTEIN; OXOTREMORINE M; QUINUCLIDINYL BENZILATE; UNCLASSIFIED DRUG;

ARTICLE; CELL STRAIN; CELL STRAIN SF9; CHO CELL; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN STABILITY; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; CELLS, CULTURED; CHO CELLS; CRICETINAE; CRICETULUS; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; HUMANS; LIGANDS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RECEPTOR, MUSCARINIC M2; RECOMBINANT FUSION PROTEINS; SEQUENCE ALIGNMENT;

EID: 79957501119     PISSN: 03044165     EISSN: 18728006     Source Type: Journal    
DOI: 10.1016/j.bbagen.2011.03.003     Document Type: Article

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