Reconstitution of β2-adrenoceptor-GTP-binding-protein interaction in Sf9 cells High coupling efficiency in a β2-adrenoceptor-G(sα) fusion protein

European Journal of Biochemistry

Volumn 255, Issue 2, 1998, Pages 369-382

  Seifert, Roland ^a   Lee, Tae Weon ^a   Lam, Van T ^a   Kobilka, Brian K ^a,b  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

2 adrenoceptor; Fusion protein; G(s) protein; Reconstitution; Sf9 cell

Indexed keywords

BETA 2 ADRENERGIC RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; HYBRID PROTEIN; 3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; ADENYLATE CYCLASE; BETA ADRENERGIC RECEPTOR; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; BETA ADRENERGIC RECEPTOR KINASE; CYCLIC AMP DEPENDENT PROTEIN KINASE; DIHYDROALPRENOLOL; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; ISOPRENALINE; PROPANOLAMINE DERIVATIVE;

ANIMAL CELL; ARTICLE; CELL LINE; CONTROLLED STUDY; GENE CONSTRUCT; IN VITRO STUDY; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; ANIMAL; ARMYWORM; BINDING COMPETITION; BIOSYNTHESIS; CELL MEMBRANE; DRUG EFFECT; GENETIC TRANSFECTION; KINETICS; METABOLISM; PHYSIOLOGY; POLYMERASE CHAIN REACTION; RAT;

ADENYLATE CYCLASE; ADRENERGIC BETA-ANTAGONISTS; ANIMALS; BETA-ADRENERGIC RECEPTOR KINASE; BINDING, COMPETITIVE; CELL LINE; CELL MEMBRANE; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DIHYDROALPRENOLOL; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; GUANOSINE 5'-O-(3-THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; ISOPROTERENOL; KINETICS; POLYMERASE CHAIN REACTION; PROPANOLAMINES; RATS; RECEPTORS, ADRENERGIC, BETA; RECOMBINANT FUSION PROTEINS; SPODOPTERA; TRANSFECTION;

EID: 0032527117     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2550369.x     Document Type: Article

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