Agonist occupation of an α(2A)-adrenoreceptor-G(i1)α fusion protein results in activation of both receptor-linked and endogenous G(i) proteins: Comparisons of their contributions to GTPase activity and signal transduction and analysis of receptor-G protein activation stoichiometry

Journal of Biological Chemistry

Volumn 273, Issue 17, 1998, Pages 10367-10375

  Burt, Andrew R ^a   Sautel, Martine ^a   Wilson, Moira A ^a   Rees, Stephen ^b   Wise, Alan ^b   Milligan, Graeme ^a,c  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENYLATE CYCLASE; ADRENALIN; ALPHA 2A ADRENERGIC RECEPTOR; BRIMONIDINE; FORSKOLIN; GUANOSINE TRIPHOSPHATASE; HYBRID PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PERTUSSIS TOXIN; ALPHA 2 ADRENERGIC RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; RECEPTOR, ADRENERGIC, ALPHA 2A; RECEPTOR, ADRENERGIC, ALPHA-2A;

AGONIST; ALPHA CHAIN; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; FIBROBLAST; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RAT; RECEPTOR AFFINITY; RECEPTOR OCCUPANCY; SIGNAL TRANSDUCTION; STOICHIOMETRY; ANIMAL; CELL LINE; COMPARATIVE STUDY; DRUG POTENTIATION; ENZYME SPECIFICITY; GENETICS; KINETICS; METABOLISM;

ANIMALS; CELL LINE; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; KINETICS; RATS; RECEPTORS, ADRENERGIC, ALPHA-2; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; SUBSTRATE SPECIFICITY;

EID: 0032562288     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.17.10367     Document Type: Article

References (37)

1. Gilman, A. G. (1987) Annu. Rev. Biochem. 56, 615-649
2. Neer, E. J. (1995) Cell 80, 249-257
3. Wise, A., Carr, I. C., and Milligan, G. (1997) Biochem. J. 325, 17-21
4. Wise, A., and Milligan, G. (1997) J. Biol. Chem. 272, 24673-24678
5. Milligan, G. (1988) Biochem. J. 255, 1-13
6. Wise, A., Watson-Koken, M.-A., Rees, S., Lee, M., and Milligan, G. (1997) Biochem. J. 321, 721-728
7. Barak., L. S., Ferguson, S. S. G., Zhang, J., Martenson, C., Meyer, T., and Caron, M. G. (1997) Mol. Pharmacol. 51, 177-184
8. Tarasova, N. I., Stauber, R. H., Choi, J. K., Hudson, E. A., Czerwinski, G., Miller, J. L., Pavlakis, G. N., Michejda, C. J., and Wank, S. A. (1997) J. Biol. Chem. 272, 14817-14824
9. Bertin, B., Jockers, R., Strosberg, A. D., and Marullo, S. (1997) Receptors Channels 5, 41-51
10. Guyer, C. A., Horstman, D. A., Wilson, A. L., Clark, J. D., Cragoe, E. J., Jr., and Limbird, L. E. (1990) J. Biol. Chem. 265, 17307-17317
11. Gillard, N. P., Linton, C. J., Milligan, G., Carr, I. C., Patmore, L., and Brown, C. M. (1996) Br. J. Pharmacol. 117, 298P
12. Wong, Y. G. (1994) Methods Enzymol. 238, 81-94
13. Mercouris, M., Mullaney, I., Georgoussi, Z., and Milligan, G. (1997) J. Neurochem. 69, 2115-2122
14. Burt, A. R., Carr, I. C., Mullaney, I., Anderson, N. G., and Milligan, G. (1996) Biochem. J. 320, 227-235
15. Wilson, M., Burt, A. R., Milligan, G., and Anderson, N. G. (1996) J. Biol. Chem. 271, 8537-8540
16. Wilson, M., Burt, A. R., Milligan, G., and Anderson, N. G. (1996) Biochem. J. 325, 217-222
17. Milligan, G., Carr, C., Gould, G. W., Mullaney, I., and Lavan, B. E. (1991) J. Biol. Chem. 266, 6447-6455
18. Wise, A., Carr, I. C., Groarke, D. A., and Milligan, G. (1997) FEBS Lett. 419, 141-146
19. Alblas, J., van Corven, E. J., Hordijk, P. L., Milligan, G., and Moolenaar, W. H. (1993) J. Biol. Chem. 268, 22235-22238
20. Anderson, N. G., and Milligan, G. (1994) Biochem. Biophys. Res. Commun. 200, 1529-1535
21. 2-adrenergic Receptors: Structure, Function and Therapeutic Implications (Lanier, S. M. and Limbird, L. E., eds) pp. 95-101, Harwood Academic Publishers, Reading, UK
22. Airriess, C. N., Rudling, J. E., Midgley, J. M., and Evans P. D. (1997) Br. J. Pharmacol. 122, 191-198
23. Eason, M. G., and Liggett, S. B. (1995) J. Biol. Chem. 270, 24753-24760
24. Eason, M. G., Kurose, H., Holt, B. D., Raymond, J. R., and Liggett, S. B. (1992) J. Biol. Chem. 267, 15795-15801
25. Yu, R., and Hinkle, P. M. (1997) Mol. Pharmacol. 51, 785-793
26. Bertin, B., Freissmuth, M., Jockers, R., Strosberg, A. D., and Marullo, S. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 8827-8831
27. Neubig, R. R. (1994) FASEB J. 8, 939-946
28. Milligan, G. (1996) Cell. Signalling 8, 87-96
29. Crespo, P., Xu, N., Simonds, W. F., and Gutkind, J. S. (1994) Nature 369, 418-420
30. Koch, W. J., Hawes, B. E., Allen, L. F., and Lefkowitz, R. J. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 12706-12710
31. van Biesen, T., Hawes, B. E., Luttrell, D. K., Krueger, K. M., Touhara, K., Porfiri, E., Sakaue, M., Luttrell, L. M., and Lefkowitz, R. J. (1995) Nature 376, 781-784
32. Chen, Y. H., Pouyssegur, J., Courtneidge, S. A., and Van Obberghen-Schilling, E. (1996) EMBO J. 15, 1037-1044
33. Kranenburg, O., Verlaan, I., Hordijk, P. L., and Moolenaar, W. H. (1997) EMBO J. 16, 3097-3105
34. Bonfini, L., Migliaccio, E., Pelicci, G., Lanfrancone, L., and Pelicci, P. (1996) Trends Biochem. Sci. 21, 257-261
35. Torres, M., and Ye, R. D. (1996) J. Biol. Chem. 271, 13244-13249
36. Wall, M. A., Coleman, D. E., Lee, E., Iniguez-Lluhi, J. A., Posner, B. A., Gilman, A. G., and Sprang, S. R. (1995) Cell 83, 1047-1058
37. Lambright, D. G., Sondek, J., Bohm, A., Skiba, N. P., Hamm, H. E., and Sigler, P. B. (1996) Nature 379, 311-319