Receptor-Gα fusion proteins as a tool for ligand screening

Life Sciences

Volumn 68, Issue 19-20, 2001, Pages 2319-2327

  Guo, Zheng Dong ^a   Suga, Hinako ^a   Okamura, Michiko ^a   Takeda, Shigeki ^a   Haga, Tatsuya ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Fusion proteins; G protein coupled receptors; Muscarinic acetylcholine receptors

Indexed keywords

BETA ADRENERGIC RECEPTOR; G PROTEIN COUPLED RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATE; HYBRID PROTEIN; LIGAND; MUSCARINIC RECEPTOR; PROTEIN SUBUNIT; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; CARBACHOL; ISOPRENALINE; VIRUS FUSION PROTEIN;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CHIMERA; PROTEIN EXPRESSION; SCREENING; ANIMAL; BACULOVIRUS; BIOSYNTHESIS; CATTLE; CELL LINE; CELL MEMBRANE; DRUG EFFECT; METABOLISM;

ADRENERGIC BETA-AGONISTS; ANIMALS; BACULOVIRIDAE; CARBACHOL; CATTLE; CELL LINE; CELL MEMBRANE; GTP-BINDING PROTEINS; ISOPROTERENOL; LIGANDS; RECEPTORS, MUSCARINIC; RECOMBINANT FUSION PROTEINS; VIRAL FUSION PROTEINS;

EID: 0035815078     PISSN: 00243205     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0024-3205(01)01021-9     Document Type: Conference Paper

References (16)

1. Bertin B, Freissmuth M, Jockers R, Strosberg AD, Marullo S. Cellular signaling by an agonist-activated receptor/Gs alpha fusion protein. Proc.Natl.Acad.Sci.U.S.A. 1994;91:8827-8831.
2. Milligan G. Insights into ligand pharmacology using receptor-G-protein fusion proteins. Trends Pharmacol.Sci. 2000;21:24-28.
3. Seifert R, Wenzel-Seifert K, Kobilka BK. GPCR-Galpha fusion proteins: Molecular analysis of receptor-G-protein coupling. Trends.Pharmacol.Sci. 1999;20:383-389.
4. Hayashi MK, Haga T. Purification and functional reconstitution with GTP-binding regulatory proteins of hexahistidine-tagged muscarinic acetylcholine receptors (m2 subtype). J.Biochem. 1996;120: 1232-1238.
5. Ikegaya T, Nishiyama T, Haga K, Haga T, Ichiyama A, Kobayashi A, Yamazaki N. Interaction of atrial muscarinic receptors with three kinds of GTP-binding proteins. J.Mol.Cell Cardiol. 1990;22: 343-351.
6. Shiozaki K, Haga T. Effects of magnesium on the interaction of atrial muscarinic acetylcholine receptors and GTP-binding regulatory proteins. Biochemistry 1992;31:10634-10642.
7. i1 alpha Fusion Protein. under submission.
8. q alpha expressed in the baculovirus-insect cell system. J.Biol.Chem. 1995;270:6246-6253.
9. 16 couple a wide variety of receptors to phospholipase C. J.Biol.Chem. 1995; 270:15175-15180.
10. i alpha. Nature 1993; 363: 274-276.
11. Fong CW, Milligan G. Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G-proteins. Biochem.J. 1999;342:457-463.
12. i 1alpha fusion protein. FEBS Lett. 1997;419:141-146.
13. i/o alpha fusion proteins. Biochemistry 1999;38:2272-2278.
14. 3 . J.Biol.Chem. 1999;274:33259-33266.
15. Quillan, JM, Hadley, ME. Ligand screening using melanophore cells:Frog skin to combinatorial chemistry. In: Haga T., Berstein G, Editors. G Protein-coupled Receptors, Boca Raton:CRC Press., 1999. pp. 71-96.
16. Receptor & ion channel nomenclature supplement. Trends.Pharmacol.Sci. 2000.