Single-molecule enzymology of chymotrypsin using water-in-oil emulsion

Biophysical Journal

Volumn 88, Issue 6, 2005, Pages 4303-4311

  Lee, Alan I ^a   Brody, James P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHYMOTRYPSIN A; SILICONE OIL; WATER;

ARTICLE; EMULSION; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE; KINETICS; MICROSCOPE; REFERENCE VALUE; STANDARD;

EID: 22244467998     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.104.055053     Document Type: Article

References (36)

1. Allen, S., S. M. Rigby-Singleton, H. Harris, M. C. Davies, and P. O'Shea. 2003. Measuring and visualizing single molecular interactions in biology. Biochem. Soc. Trans. 31:1052-1057.
2. Dyck, A. C., and D. B. Craig. 2002. Individual molecules of thermostable alkaline phosphatase support different catalytic rates at room temperature. Luminescence. 17:15-18.
3. Edman, L., Z. Földes-Papp, S. Wennmalm, and R. Rigler. 1999. The fluctuating enzyme: a single molecule approach. Chem. Phys. 247:11-22.
4. Edman, L., and R. Rigler. 2000. Memory landscapes of single-enzyme molecules. Proc. Natl. Acad. Sci. USA. 97:8266-8271.
5. Edwardson, J. M., and R. M. Henderson. 2004. Atomic force microscopy and drug discovery. Drug Discov. Today. 9:64-71.
6. Engler, N., A. Ostermann, N. Niimura, and F. G. Parak. 2003. Hydrogen atoms in proteins: positions and dynamics. Proc. Natl. Acad. Sci. USA. 100:10243-10248.
7. Földes-Papp, Z., U. Demel, and G. P. Tilz. 2004. A new concept for ultrasensitive fluorescence measurements of molecules in solution and membrane: 1. Theory and a first application. J. Immunol. Methods. 286:1-11.
8. Frauenfelder, H., B. H. McMahon, and P. W. Fenimore. 2003. Myoglobin: the hydrogen atom of biology and a paradigm of complexity. Proc. Natl. Acad. Sci. USA. 100:8615-8617.
9. Greulich, K. O. 2004. Single molecule techniques for biomedicine and pharmacology. Curr. Pharm. Biotechnol. 5:243-259.
10. Ha, T. 2001. Single-molecule fluorescence resonance energy transfer. Methods. 25:78-86,
11. Hinterdorfer, P., G. Schutz, F. Kienberger, and H. Schindler. 2001. Detection and characterization of single biomolecules at surfaces. J. Biotechnol. 82:25-35.
12. Ishii, Y., K. Kitamura, H. Tanaka, and T. Yanagida. 2003. Molecular motors and single-molecule enzymology. Methods Enzymol. 361:228-245.
13. Ishii, Y., and T. Yanagida. 2000. Single molecule detection in life science. Single Molecule. 1:5-16.
14. Ishijima, A., T. Doi, K. Sakurada, and T. Yanagida. 1991. Sub-piconewton force fluctuations of actomyosin in vitro. Nature. 352:301-306.
15. Ishijima, A., and T. Yanagida. 2001. Single molecule nanobioscience. Trends Biochem. Sci. 26:438-444.
16. Jares-Erijman, E. A., and T. M. Jovin. 2003. FRET imaging. Nat. Biotechnol. 21:1387-1395.
17. Kulzer, F., and M. Orrit. 2004. Single-molecule optics. Annu. Rev. Phys. Chem. 55:585-611.
18. Liem, L. K., J. M. Simard, Y. Song, and K. Tewari. 1995. The patch clamp technique. Neurosurgery. 36:382-392.
19. Lu, H. P., L. Xun, and X. S. Xie. 1998. Single-molecule enzymatic dynamics. Science. 282:1877-1882.
20. Medina, M. A., and P. Schwille. 2002. Fluorescence correlation spectroscopy for the detection and study of single molecules in biology. Bioessays. 24:758-764.
21. Muller, D. J., H. Janovjak, T. Lehto, L. Kuerschner, and K. Anderson. 2002. Observing structure, function and assembly of single proteins by AFM. Prog. Biophys. Mol. Biol. 79:1-43.
22. Nie, S., and R. N. Zare. 1997. Optical detection of single molecules. Annu. Rev. Biophys. Biomol. Struct. 26:567-596.
23. Noji, H., R. Yasuda, M. Yoshida, and K. Kinosita, Jr. 1997. Direct observation of the rotation of F1-ATPase. Nature. 386:299-302.
24. Park, M. K., A. V. Tepikin, and O. H. Petersen. 2002. What can we learn about cell signalling by combining optical imaging and patch clamp techniques? Pflugers Arch. 444:305-316.
25. Qian, H., and E. L. Elson. 2002. Single-molecule enzymology: stochastic Michaelis-Menten kinetics. Biophys. Chem. 101-102:565-576.
26. Rueda, D., G. Bokinsky, M. M. Rhodes, M. J. Rust, X. Zhuang, and N. G. Walter. 2004. Single-molecule enzymology of RNA: Essential functional groups impact catalysis from a distance. Proc. Natl. Acad. Sci. USA. 101: 10066-10071.
27. Sakmann, B., and E. Neher. 1984. Patch clamp techniques for studying ionic channels in excitable membranes. Annu. Rev. Physiol. 46:455-472.
28. Tan, Y. C., J. Collins, and A. P. Lee. 2003. Nanojet controlled droplet emulsion in microfluidic channels. Proc. MicroTAS Conference, Squaw Valley, CA. 963-966.
29. van den Engh, G., and C. Farmer. 1992. Photo-bleaching and photon saturation in flow cytometry. Cytometry. 13:669-677.
30. Weiss, S. 1999. Fluorescence spectroscopy of single biomolecules. Science. 283:1676-1683.
31. Weiss, S. 2000. Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy. Nat. Struct. Biol. 7:724-729.
32. Werner, J. H., H. Cai, R. A. Keller, and P. M. Goodwin. 2005. Exonuclease I hydrolyzes DNA with a distribution of rates. Biophys. J. 88:1403-1412.
33. Whitesides, G. M., E. Ostuni, S. Takayama, X. Jiang, and D. E. Ingber. 2001. Soft lithography in biology and biochemistry. Mini. Rev. Biomed. Eng. 3:335-373.
34. Xie, X. S., and H. P. Lu. 1999. Single-molecule enzymology. J. Biol. Chem. 274:15967-15970.
35. Xie, X. S., and J. K. Trautman. 1998. Optical studies of single molecules at room temperature. Annu. Rev. Phys. Chem. 49:441-480.
36. Yang, H., G. Luo, P. Kamchanaphanurach, T. M. Louie, I. Rech, S. Cova, L. Xun, and X. S. Xie. 2003. Protein conformational dynamics probed by single-molecule electron transfer. Science. 302:262-266.