Enhancement of oxidative stability of the subtilisin nattokinase by site-directed mutagenesis expressed in Escherichia coli

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1794, Issue 11, 2009, Pages 1566-1572

  Weng, MeiZhi ^a   Zheng, ZhongLiang ^a   Bao, Wei ^a   Cai, YongJun ^a   Yin, Yan ^a   Zou, GouLin ^a  

^a School of Pharmaceutical Sciences   (China)

Author keywords

E. coli; Nattokinase; Oxidative stability; Site directed mutagenesis; Subtilisin NAT

Indexed keywords

ALANINE; ASPARAGINE; HYDROGEN; HYDROGEN PEROXIDE; METHIONINE; NATTOKINASE; SERINE; SUBTILISIN; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; COMPARATIVE STUDY; CONTROLLED STUDY; CYTOPLASM; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STABILITY; ESCHERICHIA COLI; FEASIBILITY STUDY; FIBRINOLYSIS; HYDROGEN BOND; MOLECULAR DYNAMICS; MUTANT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; SIMULATION; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY; WILD TYPE;

ENZYME STABILITY; ESCHERICHIA COLI; FIBRINOLYSIS; KINETICS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; OXIDATION-REDUCTION; SUBTILISINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 70249093896     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2009.07.007     Document Type: Article

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