An image-based, high-throughput screening assay for molecules that induce excess DNA replication in human cancer cells

Molecular Cancer Research

Volumn 9, Issue 3, 2011, Pages 294-310

  Zhu, Wenge ^a,c   Lee, Chrissie Y ^a   Johnson, Ronald L ^b   Wichterman, Jennifer ^b   Huang, Ruili ^b   DePamphilis, Melvin L ^a  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

^c GEORGE WASHINGTON UNIVERSITY SCHOOL OF MEDICINE AND HEALTH SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1 [2 (4 METHOXYPHENYL) 2 [3 (4 METHOXYPHENYL)PROPOXY]ETHYL] 1H IMIDAZOLE; 2 METHOXYESTRADIOL; 2,3 DIHYDRO 2 OXO 3 (4,5,6,7 TETRAHYDRO 1H INDOL 2 YLMETHYLENE) 1H INDOLE 5 SULFONIC ACID DIMETHYLAMIDE; 3 PHENYLPROPARGYLAMINE; AMINE; AMMONIUM PYRROLIDINEDITHIOCARBAMATE; AZACITIDINE; CAFFEIC ACID PHENETHYL ESTER; CALCIMYCIN; CGP 74514A; COLCHICINE; CORTODOXONE; CYCLOSPORIN A; DIPHENYLIODONIUM SALT; DISULFIRAM; ELLIPTICINE; EMETINE; ETOPOSIDE; MEPACRINE; NOCODAZOLE; PACLITAXEL; PODOPHYLLOTOXIN; PROCHLORPERAZINE MALEATE; RESERPINE; ROTENONE; STEAROYLCARNITINE; UNCLASSIFIED DRUG; UNINDEXED DRUG; VINBLASTINE SULFATE; VINCRISTINE SULFATE; WIN 62577;

ARTICLE; BREAST CANCER; CANCER CELL; CELL NUCLEUS; CELL PROLIFERATION; COLORECTAL CANCER; CONTROLLED STUDY; DNA REPLICATION; GENE DUPLICATION; GENE INDUCTION; GENETIC SCREENING; GENOME; GENOMICS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MICROTUBULE; PRIORITY JOURNAL;

ANTINEOPLASTIC AGENTS; CELL DEATH; CELL LINE, TUMOR; CELL PROLIFERATION; DISULFIRAM; DNA REPLICATION; DRUG SCREENING ASSAYS, ANTITUMOR; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDOLES; NEOPLASMS; PARGYLINE; SMALL MOLECULE LIBRARIES; SULFONAMIDES;

EID: 79952837088     PISSN: 15417786     EISSN: 15573125     Source Type: Journal    
DOI: 10.1158/1541-7786.MCR-10-0570     Document Type: Article

References (61)

1. Ullah Z, Lee CY, Depamphilis ML. Cip/Kip cyclin-dependent protein kinase inhibitors and the road to polyploidy. Cell Div 2009;4:10.
2. DePamphilis ML, Blow JJ, Ghosh S, Saha T, Noguchi K, Vassilev A. Regulating the licensing of DNA replication origins in metazoa. Curr Opin Cell Biol 2006.
3. Sivaprasad U, Dutta A, Bell SP. Assembly of pre-replication complexes. In: DePamphilis ML, editor. DNA replication and human disease. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press; 2006. p. 63-88.
4. Zhu W, Depamphilis ML. Selective killing of cancer cells by suppression of geminin activity. Cancer Res 2009;69:4870-7.
5. Mizushina Y, Takeuchi T, Hada T, Maeda N, Sugawara F, Yoshida H, et al. The inhibitory action of SQDG (sulfoquinovosyl diacylglycerol) from spinach on Cdt1-geminin interaction. Biochimie 2008;90:947-56.
6. Mizushina Y, Takeuchi T, Takakusagi Y, Sugawara F, Sakaguchi K, Yoshida H, et al. Inhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction. Int J Mol Med 2008;21:281-90. (Pubitemid 351330478)
7. Mizushina Y, Takeuchi T, Takakusagi Y, Yonezawa Y, Mizuno T, Yanagi K, et al. Coenzyme Q10 as a potent compound that inhibits Cdt1-geminin interaction. Biochim Biophys Acta 2008;1780:203-13. (Pubitemid 351162889)
8. Inglese J, Auld DS, Jadhav A, Johnson RL, Simeonov A, Yasgar A, et al. Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries. Proc Natl Acad Sci U S A 2006;103:11473-8. (Pubitemid 44182476)
9. Bowen WP, Wylie PG. Application of laser-scanning fluorescence microplate cytometry in high content screening. Assay Drug Dev Technol 2006;4:209-21. (Pubitemid 43810925)
10. Vassilev LT, Tovar C, Chen S, Knezevic D, Zhao X, Sun H, et al. Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. Proc Natl Acad Sci U S A 2006;103:10660-5. (Pubitemid 44078033)
11. Xu H, Lv M, Tian X. A review on hemisynthesis, biosynthesis, biological activities, mode of action, and structure-activity relationship of podophyllotoxins: 2003-2007. Curr Med Chem 2009;16:327-49.
12. Zhang JH, Chung TD, Oldenburg KR. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4:67-73. (Pubitemid 29278954)
13. Hong FD, Chen J, Donovan S, Schneider N, Nisen PD. Taxol, vincristine or nocodazole induces lethality in G1-checkpoint-defective human astrocytoma U373MG cells by triggering hyperploid progression. Carcinogenesis 1999;20:1161-8. (Pubitemid 29341434)
14. Stepien A, Grzanka A, Grzanka D, Andrzej Szczepanski M, Helmin-Basa A, Gackowska L. Taxol-induced polyploidy and cell death in CHO AA8 cells. Acta Histochem 2008.
15. Caperta AD, Delgado M, Ressurreicao F, Meister A, Jones RN, Viegas W, et al. Colchicine-induced polyploidization depends on tubulin polymerization in c-metaphase cells. Protoplasma 2006;227:147-53. (Pubitemid 43807447)
16. Gustavino B, Bassani B, Pacchierotti F. Vinblastine-induced numerical chromosome changes and selection processes in mouse bone marrow cells. Mutat Res 1991;248:45-50.
17. Riffell JL, Zimmerman C, Khong A, McHardy LM, Roberge M. Effects of chemical manipulation of mitotic arrest and slippage on cancer cell survival and proliferation. Cell Cycle 2009;8:3025-38.
18. Lee J, Kim JA, Margolis RL, Fotedar R. Substrate degradation by the anaphase promoting complex occurs during mitotic slippage. Cell Cycle 2010;9:1792-801.
19. Xu D, Bai J, Duan Q, Costa M, Dai W. Covalent modifications of histones during mitosis and meiosis. Cell Cycle 2009;8:3688-94.
20. Elhajouji A, Cunha M, Kirsch-Volders M. Spindle poisons can induce polyploidy by mitotic slippage and micronucleate mononucleates in the cytokinesis-block assay. Mutagenesis 1998;13:193-8. (Pubitemid 28138305)
21. Meek DW. The role of p53 in the response to mitotic spindle damage. Pathol Biol (Paris). 2000;48:246-54. (Pubitemid 30318125)
22. Chou AP, Li S, Fitzmaurice AG, Bronstein JM. Mechanisms of rotenone-induced proteasome inhibition. Neurotoxicology 2010;31:367-72.
23. Srivastava P, Panda D. Rotenone inhibits mammalian cell proliferation by inhibiting microtubule assembly through tubulin binding. FEBS J 2007;274:4788-801. (Pubitemid 47353128)
24. Verdier-Pinard P, Wang Z, Mohanakrishnan AK, Cushman M, Hamel E. A steroid derivative with paclitaxel-like effects on tubulin polymerization. Mol Pharmacol 2000;57:568-75. (Pubitemid 30140888)
25. Lin HL, Liu TY, Chau GY, Lui WY, Chi CW. Comparison of 2-methoxyestradiol-induced, docetaxel-induced, and paclitaxel-induced apoptosis in hepatoma cells and its correlation with reactive oxygen species. Cancer 2000;89:983-94.
26. Behrend L, MilneDM, Stoter M,DeppertW,Campbell LE, Meek DW, et al. IC261, a specific inhibitor of the protein kinases casein kinase 1-delta and -epsilon, triggers the mitotic checkpoint and induces p53-dependent postmitotic effects. Oncogene 2000;19:5303-13.
27. Ma HT, Tsang YH, Marxer M, Poon RY. Cyclin A2-cyclin-dependent kinase 2 cooperates with the PLK1-SCFbeta-TrCP1-EMI1-anaphase-promoting complex/cyclosome axis to promote genome reduplication in the absence of mitosis. Mol Cell Biol 2009;29:6500-14.
28. Ullah Z, Kohn MJ, Yagi R, Vassilev LT, DePamphilis ML. Differentiation of trophoblast stem cells into giant cells is triggered by p57/Kip2 inhibition of CDK1 activity. Genes Dev 2008;22:3024-36.
29. Dai Y, Dent P, Grant S. Induction of apoptosis in human leukemia cells by the CDK1 inhibitor CGP74514A. Cell Cycle 2002;1:143-52.
30. Reiners JJ Jr, Kleinman M, Joiakim A, Mathieu PA. The chemotherapeutic agents XK469 (2-{4-[(7-chloro-2-quinoxalinyl)oxy]phenoxy} propionic acid) and SH80 (2-{4-[(7-bromo-2-quinolinyl)oxy]phenoxy} propionic acid) inhibit cytokinesis and promote polyploidy and induce senescence. J Pharmacol Exp Ther 2009;328:796-806.
31. Rello-Varona S, Gamez A, Moreno V, Stockert JC, Cristobal J, Pacheco M, et al. Metaphase arrest and cell death induced by etoposide on HeLa cells. Int J Biochem Cell Biology 2006;38:2183-95. (Pubitemid 44397625)
32. Sumner AT. Induction of diplochromosomes in mammalian cells by inhibitors of topoisomerase II. Chromosoma 1998;107:486-90. (Pubitemid 29057542)
33. Santidrian AF, Cosialls AM, Coll-Mulet L, Iglesias-Serret D, de Frias M, Gonzalez-Girones DM, et al. The potential anticancer agent PK11195 induces apoptosis irrespective of p53 and ATM status in chronic lymphocytic leukemia cells. Haematologica 2007;92:1631-8. (Pubitemid 350248242)
34. Shoukrun R, Veenman L, Shandalov Y, Leschiner S, Spanier I, Karry R, et al. The 18-kDa translocator protein, formerly known as the peripheral-type benzodiazepine receptor, confers proapoptotic and antineoplastic effects in a human colorectal cancer cell line. Pharmacogenet Genomics 2008;18:977-88.
35. Chen D, Cui QC, Yang H, Dou QP. Disulfiram, a clinically used antialcoholism drug and copper-binding agent, induces apoptotic cell death in breast cancer cultures and xenografts via inhibition of the proteasome activity. Cancer Res 2006;66:10425-33. (Pubitemid 44799763)
36. Iljin K, Ketola K, Vainio P, Halonen P, Kohonen P, Fey V, et al. Highth-roughput cell-based screening of 4910 known drugs and drug-like small molecules identifies disulfiram as an inhibitor of prostate cancer cell growth. Clin Cancer Res 2009;15:6070-8.
37. Martin SS, Leder P. Human MCF10A mammary epithelial cells undergo apoptosis following actin depolymerization that is independent of attachment and rescued by Bcl-2. Mol Cell Biol 2001;21:6529-36. (Pubitemid 32927420)
38. Ishizawar R, Parsons SJ. c-Src and cooperating partners in human cancer. Cancer cell 2004;6:209-14. (Pubitemid 39222033)
39. Thomas SM, Brugge JS. Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 1997;13:513-609. (Pubitemid 28034534)
40. Blake RA, Broome MA, Liu X, Wu J, Gishizky M, Sun L, et al. SU6656, a selective src family kinase inhibitor, used to probe growth factor signaling. Mol Cell Biol 2000;20:9018-27. (Pubitemid 32245931)
41. Bain J, Plater L, Elliott M, Shpiro N, Hastie CJ, McLauchlan H, et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 2007;408:297-315.
42. Dussault N, Simard C, Neron S, Cote S. Human B lymphocytes and non-Hodgkin's lymphoma cells become polyploid in response to the protein kinase inhibitor SU6656. Blood Cells Mol Dis 2007;39:130-4. (Pubitemid 46754925)
43. Lannutti BJ, Blake N, Gandhi MJ, Reems JA, Drachman JG. Induction of polyploidization in leukemic cell lines and primary bone marrow by Src kinase inhibitor SU6656. Blood 2005;105:3875-8. (Pubitemid 40656131)
44. Talos F, Moll UM. Role of the p53 family in stabilizing the genome and preventing polyploidization. Adv Exp Med Biol 2010;676:73-91.
45. Casenghi M, Mangiacasale R, Tuynder M, Caillet-Fauquet P, Elhajouji A, Lavia P, et al. p53-independent apoptosis and p53-dependent block of DNA rereplication following mitotic spindle inhibition in human cells. Exp Cell Res 1999;250:339-50. (Pubitemid 29367445)
46. Di Leonardo A, Khan SH, Linke SP, Greco V, Seidita G, Wahl GM. DNA rereplication in the presence of mitotic spindle inhibitors in human and mouse fibroblasts lacking either p53 or pRb function. Cancer Res 1997;57:1013-9. (Pubitemid 27138591)
47. Wahl AF, Donaldson KL, Fairchild C, Lee FY, Foster SA, Demers GW, et al. Loss of normal p53 function confers sensitization to Taxol by increasing G2/M arrest and apoptosis. Nat Med 1996;2:72-9. (Pubitemid 26029571)
48. Tsuiki H, Nitta M, Tada M, Inagaki M, Ushio Y, Saya H. Mechanism of hyperploid cell formation induced by microtubule inhibiting drug in glioma cell lines. Oncogene 2001;20:420-9. (Pubitemid 32158222)
49. Yan W, Liu G, Scoumanne A, Chen X. Suppression of inhibitor of differentiation 2, a target of mutant p53, is required for gain-offunction mutations. Cancer Res 2008;68:6789-96.
50. Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown JP, et al. Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science 1998;282:1497-501. (Pubitemid 28538613)
51. Kavallaris M. Microtubules and resistance to tubulin-binding agents. Nat Rev Cancer 2010;10:194-204.
52. Ullah Z, Lee CY, Lilly MA, DePamphilis ML. Developmentally programmed endoreduplication in animals. Cell Cycle 2009;8:1501-9.
53. Matei D, Schilder J, Sutton G, Perkins S, Breen T, Quon C, et al. Activity of 2 methoxyestradiol (Panzem NCD) in advanced, platinumresistant ovarian cancer and primary peritoneal carcinomatosis: a Hoosier Oncology Group trial. Gynecol Oncol 2009;115:90-6.
54. Tevaarwerk AJ, Holen KD, Alberti DB, Sidor C, Arnott J, Quon C, et al. Phase I trial of 2-methoxyestradiol NanoCrystal dispersion in advanced solid malignancies. Clin Cancer Res 2009;15:1460-5.
55. Reiner T, de las Pozas A, Gomez LA, Perez-Stable C. Low dose combinations of 2-methoxyestradiol and docetaxel block prostate cancer cells in mitosis and increase apoptosis. Cancer Lett 2009;276:21-31.
56. Brar SS, Grigg C, Wilson KS, Holder WD Jr, Dreau D, Austin C, et al. Disulfiram inhibits activating transcription factor/cyclic AMP-responsive element binding protein and human melanoma growth in a metaldependent manner in vitro, in mice and in a patient with metastatic disease. Mol Cancer Ther 2004;3:1049-60. (Pubitemid 39295002)
57. Lipsky JJ, Shen ML, Naylor S. Overview - in vitro inhibition of aldehyde dehydrogenase by disulfiram and metabolites. Chem Biol Interact 2001;130-132:81-91. (Pubitemid 32295805)
58. Yakisich JS, Siden A, Eneroth P, Cruz M. Disulfiram is a potent in vitro inhibitor of DNA topoisomerases. Biochem Biophys Res Commun 2001;289:586-90. (Pubitemid 33143819)
59. Brahemi G, Kona FR, Fiasella A, Buac D, Soukupova J, Brancale A, et al. Exploring the structural requirements for inhibition of the ubiquitin E3 ligase breast cancer associated protein 2 (BCA2) as a treatment for breast cancer. J Med Chem 2010;53:2757-65.
60. Lovborg H, Oberg F, Rickardson L, Gullbo J, Nygren P, Larsson R. Inhibition of proteasome activity, nuclear factor-KappaB translocation and cell survival by the antialcoholism drug disulfiram. Int J Cancer 2006;118:1577-80.
61. Yamaguchi R, Dutta A. Proteasome inhibitors alter the orderly progression of DNA synthesis during S-phase in HeLa cells and lead to rereplication of DNA. Exp Cell Res 2000;261:271-83.