Substitution of active site tyrosines with tryptophan alters the free energy for nucleotide flipping by human alkyladenine DNA glycosylase

Biochemistry

Volumn 50, Issue 11, 2011, Pages 1864-1874

  Hendershot, Jenna M ^a   Wolfe, Abigail E ^a   O'Brien, Patrick J ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACTIVE SITE POCKET; BASE EXCISION REPAIRS; BASE PAIRS; DELETERIOUS EFFECTS; DNA BINDING; DNA GLYCOSYLASE; GLYCOSYLASE; KINETIC EFFECT; MUTANT PROTEINS; PURINE LESION; STOPPED FLOW; TIME-DEPENDENT CHANGES; TRYPTOPHAN FLUORESCENCE; TYROSINE RESIDUES; WILD TYPES; WILD-TYPE ENZYMES;

AMINO ACIDS; BINDING ENERGY; DNA; EQUILIBRIUM CONSTANTS; FLUORESCENCE; NUCLEIC ACIDS; NUCLEOTIDES; QUENCHING;

RATE CONSTANTS;

ALKYLADENINE DNA GLYCOSYLASE; DNA GLYCOSYLTRANSFERASE; NUCLEOTIDE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DNA BINDING; ENERGY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; FLUORESCENCE; HUMAN; KINETICS; MOLECULAR RECOGNITION; MUTATION; NONHUMAN; PRIORITY JOURNAL; SYNTHESIS; WILD TYPE;

BASE PAIRING; CATALYTIC DOMAIN; DNA; DNA GLYCOSYLASES; HUMANS; KINETICS; NUCLEOTIDES; SUBSTRATE SPECIFICITY; THERMODYNAMICS; TRYPTOPHAN; TYROSINE;

EID: 79952812338     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi101856a     Document Type: Article

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