Physical association of PDK1 with AKT1 Is sufficient for pathway activation independent of membrane localization and phosphatidylinositol 3 kinase

PLoS ONE

Volumn 5, Issue 3, 2010, Pages

  Ding, Zhiyong ^a   Liang, Jiyong ^a   Li, Jin ^a   Lu, Yiling ^a   Ariyaratna, Vathsala ^a   Lu, Zhimin ^a   Davies, Michael A ^a   Westwick, John K ^b   Mills, Gordon B ^a  

^a UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

^b Odyssey Thera Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; PROTEIN SERINE THREONINE KINASE; PYRUVATE DEHYDROGENASE (ACETYL TRANSFERRING) KINASE; PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING) KINASE; SMALL INTERFERING RNA; THREONINE;

ARTICLE; CELL MEMBRANE; CELL SURVIVAL; CHEMISTRY; GENE EXPRESSION REGULATION; HELA CELL; HUMAN; METABOLISM; MUTATION; PHOSPHORYLATION; PROTEIN BINDING;

CELL MEMBRANE; CELL SURVIVAL; FLUORESCENT DYES; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; MUTATION; PHOSPHATIDYLINOSITOL 3-KINASES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS C-AKT; RNA, SMALL INTERFERING; THREONINE;

EID: 79952117054     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0009910     Document Type: Article

References (32)

1. Brazil DP, Yang ZZ, Hemmings BA (2004) Advances in protein kinase B signalling: AKTion on multiple fronts. Trends Biochem Sci 29: 233-242.
2. Manning BD, Cantley LC (2007) AKT/PKB signaling: navigating downstream. Cell 129: 1261-1274.
3. Hennessy BT, Smith DL, Ram PT, Lu Y, Mills GB (2005) Exploiting the PI3K/ AKT pathway for cancer drug discovery. Nat Rev Drug Discov 4: 988-1004.
4. Alessi DR, James SR, Downes CP, Holmes AB, Gaffney PR, et al. (1997) Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha. Curr Biol 7: 261-269.
5. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM (2005) Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307: 1098-1101.
6. Andjelkovic M, Alessi DR, Meier R, Fernandez A, Lamb NJ, et al. (1997) Role of Translocation in the Activation and Function of Protein Kinase B. J Biol Chem 272: 31515-31524.
7. Calleja V, Alcor D, Laguerre M, Park J, Vojnovic B, et al. (2007) Intramolecular and intermolecular interactions of protein kinase B define its activation in vivo. PLoS Biol 5: e95.
8. Calleja V, Laguerre M, Parker PJ, Larijani B (2009) Role of a novel PH-kinase domain interface in PKB/Akt regulation: structural mechanism for allosteric inhibition. PLoS Biol 7: e17.
9. Higuchi M, Onishi K, Kikuchi C, Gotoh Y (2008) Scaffolding function of PAK in the PDK1-Akt pathway. Nat Cell Biol 10: 1356-1364.
10. Nakamura A, Naito M, Tsuruo T, Fujita N (2008) Freud-1/Aki1, a novel PDK1-interacting protein, functions as a scaffold to activate the PDK1/Akt pathway in epidermal growth factor signaling. Mol Cell Biol 28: 5996-6009.
11. Yang WL, Wang J, Chan CH, Lee SW, Campos AD, et al. (2009) The E3 ligase TRAF6 regulates Akt ubiquitination and activation. Science 325: 1134-1138.
12. Ding Z, Liang J, Lu Y, Yu Q, Songyang Z, et al. (2006) A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners. PNAS 103: 15014-15019.
13. Giepmans BN, Adams SR, Ellisman MH, Tsien RY (2006) The fluorescent toolbox for assessing protein location and function. Science 312: 217-224.
14. Kerppola TK (2006) Visualization of molecular interactions by fluorescence complementation. Nat Rev Mol Cell Biol.
15. Macdonald ML, Lamerdin J, Owens S, Keon BH, Bilter GK, et al. (2006) Identifying off-target effects and hidden phenotypes of drugs in human cells. Nat Chem Biol.
16. Magliery TJ, Wilson CG, Pan W, Mishler D, Ghosh I, et al. (2005) Detecting protein-protein interactions with a green fluorescent protein fragment reassembly trap: scope and mechanism. J Am Chem Soc 127: 146-157.
17. Nagai T, Ibata K, Park ES, Kubota M, Mikoshiba K, et al. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20: 87-90.
18. Michnick SW, Remy I, Campbell-Valois FX, Vallee-Belisle A, Pelletier JN (2000) Detection of protein-protein interactions by protein fragment complementation strategies. Methods Enzymol 328: 208-230.
19. Anderson KE, Coadwell J, Stephens LR, Hawkins PT (1998) Translocation of PDK-1 to the plasma membrane is important in allowing PDK-1 to activate protein kinase B. Curr Biol 8: 684-691.
20. Saji M, Vasko V, Kada F, Allbritton EH, Burman KD, et al. (2005) Akt1 contains a functional leucine-rich nuclear export sequence. Biochem Biophys Res Commun 332: 167-173.
21. Scheid MP, Parsons M, Woodgett JR (2005) Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation. Mol Cell Biol 25: 2347-2363.
22. Jacinto E, Facchinetti V, Liu D, Soto N, Wei S, et al. (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127: 125-137.
23. Bellacosa A, Chan TO, Ahmed NN, Datta K, Malstrom S, et al. (1998) Akt activation by growth factors is a multiple-step process: the role of the PH domain. Oncogene 17: 313-325.
24. Knight ZA, Gonzalez B, Feldman ME, Zunder ER, Goldenberg DD, et al. (2006) A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling. Cell 125: 733-747.
25. Folkes AJ, Ahmadi K, Alderton WK, Alix S, Baker SJ, et al. (2008) The identification of 2-(1H-indazol-4-yl)-6-(4-methanesulfonyl-piperazin-1-ylmethyl)-4-morpholin-4-yl-thieno[3,2-d]pyrimidine (GDC-0941) as a potent, selective, orally bioavailable inhibitor of class I PI3 kinase for the treatment of cancer. J Med Chem 51: 5522-5532.
26. Barnett SF (2005) Identification and characterization of pleckstrin-homologydomain-dependent and isoenzyme-specific Akt inhibitors. Biochem J 385: 399-408.
27. Defeo-Jones D (2005) Tumor cell sensitization to apoptotic stimuli by selective inhibition of specific Akt/PKB family members. Mol Cancer Ther 4: 271-279.
28. Bayascas JR, Wullschleger S, Sakamoto K, Garcia-Martinez JM, Clacher C, et al. (2008) Mutation of the PDK1 PH Domain Inhibits Protein Kinase B/Akt, Leading to Small Size and Insulin Resistance. Mol Cell Biol 28: 3258-3272.
29. Lahair MM, Howe CJ, Rodriguez-Mora O, McCubrey JA, Franklin RA (2006) Molecular pathways leading to oxidative stress-induced phosphorylation of Akt. Antioxid Redox Signal 8: 1749-1756.
30. Howe CJ, LaHair MM, Maxwell JA, Lee JT, Robinson PJ, et al. (2002) Participation of the Calcium/Calmodulin-dependent Kinases in Hydrogen Peroxide-induced Ikappa B Phosphorylation in Human T Lymphocytes. J Biol Chem 277: 30469-30476.
31. Yano S, Tokumitsu H, Soderling TR (1998) Calcium promotes cell survival through CaM-K kinase activation of the protein-kinase-B pathway. Nature 396: 584-587.
32. Lu Y, Yu Q, Liu JH, Zhang J, Wang H, et al. (2003) Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/ AKT cascades. J Biol Chem 278: 40057-40066.