Improving a natural enzyme activity through incorporation of unnatural amino acids

Journal of the American Chemical Society

Volumn 133, Issue 2, 2011, Pages 326-333

  Ugwumba, Isaac N ^a,b   Ozawa, Kiyoshi ^b,c   Xu, Zhi Qiang ^a,c   Ely, Fernanda ^d   Foo, Jee Loon ^b   Herlt, Anthony J ^b   Coppin, Chris ^a   Brown, Sue ^a   Taylor, Matthew C ^a   Ollis, David L ^b   Mander, Lewis N ^b   Schenk, Gerhard ^b,e   Dixon, Nicholas E ^d   Otting, Gottfried ^b   Oakeshott, John G ^a   Jackson, Colin J ^a,b  

^a CSIRO   (Australia)

^b AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^c UNIVERSITY OF WOLLONGONG   (Australia)

^d UNIVERSITY OF QUEENSLAND   (Australia)

^e NATIONAL UNIVERSITY OF IRELAND   (Ireland)

Author keywords

[No Author keywords available]

Indexed keywords

7-HYDROXYCOUMARIN; ACTIVE SITE RESIDUES; CATALYTIC CYCLES; EFFICIENT CATALYSTS; ELECTROSTATIC REPULSION; ENZYME FUNCTIONS; EVOLUTIONARY LIMITS; FUNCTIONAL SPACES; HYDROXYL GROUPS; KINETIC ANALYSIS; NATURAL AMINO ACIDS; NATURALLY OCCURRING; PARAOXON; PHOSPHOTRIESTERASES; PRODUCT RELEASE; RATE LIMITING; SUBSTRATE BINDING; TURNOVER RATE;

ENZYME ACTIVITY; ENZYMES; HYDROLYSIS;

AMINO ACIDS;

(7 HYDROXYCOUMARIN 4 YL)ETHYLGLYCINE; (7 METHYLCOUMARIN 4 YL)ETHYLGLYCINE; GLYCINE DERIVATIVE; LEUCINE; PARAOXON; PHENOL DERIVATIVE; PHENYLALANINE; PHOSPHATE; PHOSPHOTRIESTERASE; TRYPTOPHAN; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYST; COMPUTER SIMULATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENE MUTATION; HYDROLYSIS; NONHUMAN; PH; PROTEIN EXPRESSION; SCREENING; STATIC ELECTRICITY; TURNOVER TIME;

AMINO ACIDS; BIOCATALYSIS; ENZYME ACTIVATION; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; MODELS, MOLECULAR; MOLECULAR STRUCTURE; PARAOXON; PHOSPHORIC TRIESTER HYDROLASES;

EID: 78651389634     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja106416g     Document Type: Article

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