Improving nature's enzyme active site with genetically encoded unnatural amino acids

Journal of the American Chemical Society

Volumn 128, Issue 34, 2006, Pages 11124-11127

  Jackson, Jennifer C ^a   Duffy, Sean P ^a   Hess, Kenneth R ^a   Mehl, Ryan A ^a  

^a FRANKLIN AND MARSHALL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROSTATICS; ENZYME KINETICS; GENETIC ENGINEERING; MOLECULAR STRUCTURE;

ELECTROSTATIC DIVERSITY; NITROREDUCTASE ACTIVITY; PRODRUG ACTIVATOR NITROREDUCTASE;

AMINO ACIDS;

AMINO ACID; NITROREDUCTASE;

ARTICLE; DNA CROSS LINKING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; GENETIC CODE; MUTAGENESIS; PROTEIN FUNCTION; PROTEIN MODIFICATION; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION;

AMINO ACIDS; BASE SEQUENCE; BINDING SITES; DNA PRIMERS; ELECTROSTATICS; ENZYMES; MODELS, MOLECULAR; TANDEM MASS SPECTROMETRY;

EID: 33748053033     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja061099y     Document Type: Article

References (13)

1. Wang, L.; Brock, A.; Herberich, A.; Schultz, P. G. Science 2001, 292, 498-00.
2. Mehl, R. A.; Anderson, J. C.; Santoro, S. W.; Wang, L.; Martin, A. B.; King, D. S.; Horn, D. M.; Schultz, P. G. J. Am. Chem. Soc. 2003, 125, 935-9.
3. Xie, J.; Schultz, P. G. Methods 2005, 36(3), 227-38.
4. Xie, J.; Schultz, P. G. Curr. Opin. Chem. Biol. 2005, 9, 548-54.
5. Judice, J. K.; Gramble, T. R.; Murphy, E. C.; de Vos, A. M.; Schultz, P. G. Science 1993, 261, 1578-81.
6. Gloss, L. M.; Kirsch, J. F. Biochemistry 1995, 34, 12323-32.
7. Grove, J. I.; Lovering, A. L.; Guise, C.; Race, P. R.; Wrighton, C. J.; White, S. A.; Hyde, E. I.; Searle, P. F. Cancer Res. 2003, 63, 5532-7.
8. Hu, L.; Yu, C.; Jiang, Y.; Han, J.; Li, Z.; Browne, P.; Race, P. R.; Knox, R. J.; Searle, P. F.; Hyde, E. I. J. Med. Chem. 2003, 46, 4818-21.
9. Lovering, A. L.; Hyde, E. I.; Searle, P. F.; White, S. A. J. Mol. Biol. 2001, 309, 203-13.
10. Chin, J. W.; Martin, A. B.; King, D. S.; Wang, L.; Schultz, P. G. Proc. Natl. Acad. Sci. USA 2002, 99, 11020-4.
11. Wang, L.; Brock, A.; Schultz, P. G. J. Am. Chem. Soc. 2002, 124, 1836.
12. Jackson, J. C.; Mehl, R. A. Site-specific incorporation of a 19F-aminoacid into proteins as an NMR probe for characterizing protein structure and reactivity. J. Am. Chem. Soc. 2006, submitted.
13. Farrell, I. S.; Toroney, R.; Hazen, J. L.; Mehl, R. A.; Chin, J. W. Nat. Methods 2005, 2, 377-84.