Mutation of outer-shell residues modulates metal ion co-ordination strength in a metalloenzyme

Biochemical Journal

Volumn 429, Issue 2, 2010, Pages 313-321

  Foo, Jee Loon ^a   Jackson, Colin J ^b   Carr, Paul D ^a   Kim, Hye Kyung ^a   Schenk, Gerhard ^c   Gahan, Lawrence R ^c   Ollis, David L ^a  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b CNRS   (France)

^c UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Directed evolution; Metal ion binding; Metal ion co ordination; Phosphotriesterase (PTE)

Indexed keywords

ACTIVE SITE; AGROBACTERIUM RADIOBACTER; BIOCHEMICAL PROPERTIES; CONFORMATIONAL STATE; DIRECTED EVOLUTION; ESSENTIAL SUPPORT; METAL ION BINDING; METAL ION INTERACTIONS; METALLO-PROTEINS; METALLOENZYMES; MODEL SYSTEM; OPTIMAL ORIENTATION; ORTHOLOGUES; PHOSPHOTRIESTERASES; PROTEIN FOLDS; PSEUDOMONAS DIMINUTA; SMALL MOLECULES; SPECTROSCOPIC TECHNIQUE; STABILIZING EFFECTS; STRUCTURAL DATA; STRUCTURAL DETERMINANTS;

METALS; PROTEIN FOLDING; X RAY CRYSTALLOGRAPHY;

METAL IONS;

METAL ION; PHOSPHOTRIESTERASE; METALLOPROTEIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DISSOCIATION; IONIC STRENGTH; METAL BINDING; MUTATION; PRIORITY JOURNAL; PROTEIN FOLDING; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY; CHEMICAL STRUCTURE; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PROTEIN CONFORMATION; PSEUDOMONAS; RHIZOBIUM;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS); BREVUNDIMONAS DIMINUTA;

CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; KINETICS; METALLOPROTEINS; MODELS, MOLECULAR; MUTATION; PHOSPHORIC TRIESTER HYDROLASES; PROTEIN CONFORMATION; PSEUDOMONAS; RHIZOBIUM;

EID: 77954739001     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20100233     Document Type: Article

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