Impact of plasmid induction strategy on overall plasmid DNA yield and E. coli physiology using flow cytometry and real-time PCR

Process Biochemistry

Volumn 46, Issue 1, 2011, Pages 174-181

  Silva, Filomena ^a   Loureno, Olga ^a   Maia, Cláudio ^a   Queiroz, João A ^a   Domingues, Fernanda C ^a  

^a UNIVERSITY OF BEIRA INTERIOR   (Portugal)

Author keywords

Amino acid limitation; Flow cytometry; Plasmid DNA; Real time qPCR; Temperature up shift

Indexed keywords

CELL PHYSIOLOGY; COPY NUMBER; E. COLI; FERMENTATION PROCESS; FILAMENTATION; IN-VIVO; INDUCTION STRATEGIES; MONITORING TECHNIQUES; MULTI-PARAMETER FLOW CYTOMETRY; NAKED DNA; PLASMID DNA; PROCESS PRODUCTIVITY; REAL-TIME PCR; REAL-TIME QPCR; SPECIFIC YIELD;

AMINO ACIDS; BIOMOLECULES; CYTOLOGY; DNA; ESCHERICHIA COLI; FERMENTATION; FLOW CYTOMETRY; GENE EXPRESSION; GENE THERAPY; GENETIC ENGINEERING; METABOLISM; OPTIMIZATION; PHYSIOLOGY; PRODUCTIVITY;

POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 78650243570     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.08.001     Document Type: Article

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