Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR

Molecular Biotechnology

Volumn 37, Issue 2, 2007, Pages 120-126

  Carapuça, Elisabete ^a   Azzoni, Adriano R ^a   Prazeres, Duarte M F ^a   Monteiro, Gabriel A ^a   Mergulhão, Filipe J M ^a,b  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^b UNIVERSITY OF PORTO   (Portugal)

Author keywords

Eukaryotic and prokaryotic cells; Plasmid copy number; Plasmid DNA decay; Plasmid DNA vectors; Real Time PCR; SYBR Green

Indexed keywords

GREEN FLUORESCENT PROTEIN (GFP) REPORTER; PLASMID COPY NUMBERS; PLASMID DNA DECAY; PLASMID DNA VECTORS; REAL TIME PCR;

BATCH CELL CULTURE; DNA; ESCHERICHIA COLI; PROTEINS; REAL TIME SYSTEMS; VACCINES;

MOLECULAR BIOLOGY;

DNA VACCINE; PROTEIN 15A; PROTEIN P15; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; DNA IMMUNIZATION; ESCHERICHIA COLI; EUKARYOTIC CELL; GENETIC ANALYSIS; GENETIC TRANSFECTION; NONHUMAN; PLASMID; PROKARYOTIC CELL; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; ESCHERICHIA COLI; GENE DOSAGE; KINETICS; PLASMIDS; POLYMERASE CHAIN REACTION; TIME FACTORS;

CRICETULUS GRISEUS; ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

EID: 34248999538     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-007-0007-3     Document Type: Article

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