Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA

Biotechnology Progress

Volumn 22, Issue 4, 2006, Pages 1179-1186

  Freitas, S S ^a   Santos, J A L ^a   Prazeres, D M F ^a  

^a INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOLS; AMMONIUM COMPOUNDS; CONCENTRATION (PROCESS); IMPURITIES; OPTIMIZATION; REMOVAL; RNA;

DOWNSTREAM PROCESSING; PDNA PELLETS; PRECIPITATION TECHNIQUES; PROCESS ECONOMICS;

DNA;

2 PROPANOL; AMMONIUM SULFATE; DNA;

ARTICLE; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; ISOLATION AND PURIFICATION; PLASMID; PRECIPITATION;

2-PROPANOL; AMMONIUM SULFATE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA; FRACTIONAL PRECIPITATION; HYDROPHOBICITY; PLASMIDS; PRECIPITATION;

ESCHERICHIA COLI;

EID: 33747166436     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp060052d     Document Type: Article

References (25)

1. Edelstein, M. L.; Abedi, M. R.; Wixon, J.; Edelstein, R. M. Gene therapy clinical trials worldwide 1989-2004-an overview. J. Gene Med. 2004, 6, 597-602.
2. Costa, S. M.; Freire, M. S.; Alves, A. M. DNA vaccine against the nonstructural 1 protein (NS1) of dengue 2 virus. Vaccine 2005, in press.
3. Zhu, D.; Jiang, S.; Luo, X. Therapeutic effects of Ag85B and MPT64 DNA vaccines in a murine model of Mycobacterium tuberculosis infection. Vaccine 2005, 23, 4619-4624.
4. Prather, K. J.; Sagar, S.; Murphy, J.; Chartrain, M. Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification. Enzyme Microb. Technol. 2003, 33, 865-883.
5. Prazeres, D. M. F.; Ferreira, G. N. M.; Monteiro, G. A.; Cooney, C. L.; Cabral, J. M. S. Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: problems and bottlenecks. Trends Biotechnol. 1999, 17, 169-174.
6. Shamlou, P. A. Scaleable processes for the manufacture of therapeutic quantities of plasmid DNA. Biotechnol. Appl. Biochem. 2003, 37, 207-218.
7. Varley, D. L.; Hitchkock, A. G.; Weiss, A. M. E.; Horler, W. A.; Cowell, R.; Peddie, L.; Sharpe, G. S.; Thatcher, D. R.; Hanak, J. A. J. Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography. Bioseparation 1998, 8, 209-217.
8. Ferreira, G. N. M.; Cabral, J. M. S.; Prazeres, D. M. F. Development of process flow sheets for the purification of plasmid vectors for gene therapy applications. Biotechnol. Prog. 1999, 15, 725-731.
9. Diogo, M. M.; Queiroz, J. A.; Monteiro, G. A.; Martins, S. A. M.; Ferreira, G. N. M.; Prazeres, D. M. F. Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography. Biotechnol. Bioeng. 2000, 68, 576-583.
10. Horn, N. A.; Meek, J. A.; Budahazi, G.; Marquet, M. Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials. Hum. Gene Ther. 1995, 6, 565-573.
11. Murphy, J. C.; Wibbenmeyer, J. A.; Fox, G. E.; Willson, R. C. Purification of plasmid DNA using selective precipitation by compacting agents. Nat. Biotechnol. 1999, 17, 822-823.
12. Lander, R. J.; Winters, M. A.; Meacle, F. J.; Buckland, B. C.; Lee, A. L. Fractional precipitation of plasmid DNA from lysate by CTAB. Biotechnol. Bioeng. 2002, 79, 776-784.
13. Eon-Duval, A.; Gumbs, K.; Ellett, C. Precipitation of RNA impurities with high salt in a plasmid DNA purification process: use of experimental design to determine reaction conditions. Biotechnol. Bioeng. 2003, 83, 544-553.
14. Diogo, M. M.; Ribeiro, S. C.; Queiroz, J. A.; Monteiro, G. A.; Tordo, N.; Perrin, P.; Prazeres, D. M. F. Production, purification and analysis of an experimental DNA vaccine against rabies. J. Gene Med. 2001, 3, 577-584.
15. Freitas, S. S.; Santos, J. A. L.; Prazeres, D. M. F. In Development of Sustainable Bioprocesses: Modeling and Assessment; Biwer, A., Heinzle, E., Cooney, C., Eds.; Wiley: New York, 2006; in press.
16. Biwer, A.; Heinzle, E. Environmental assessment in early process development. J. Chem. Technol. Biotechnol. 2004, 79, 597-609.
17. Scopes, R. K. Protein purification: principles and practice; Springer-Verlag: New York, 1982.
18. Box, G. E. P.; Hunter, W. G.; Hunter, J. S. Statistics for experiments: an introduction to design, data analysis and model building; Wiley: New York, 1978.
19. Diogo, M. M.; Queiroz, J. A.; Prazeres, D. M. F. Assessment of purity and quantification of plasmid DNA in process solutions using high performance hydrophobic interaction chromatography. J. Chromatogr., A 2003, 998, 109-117.
20. Martins, S. A.; Prazeres, D. M.; Cabral, J. M.; Monteiro, G. A. Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products. Anal. Biochem. 2003, 522, 127-129.
21. Arscott, P. G.; Ma, C.; Wenner, J. R.; Bloomfield, V. A. DNA condensation by cobalt hexaammine(III) in alcohol-water mixtures: dielectric constant and other solvent effects. Biopolymers 1995, 36, 345-364.
22. Flock, S.; Labarbe, R.; Houssier, C. Dielectric constant and ionic strength effects on DNA precipitation. Biophys. J. 1996, 70, 1456-1465.
23. Tseng, W.-C.; Ho, F.-L. Enhanced purification of plasmid DNA using Q-Sepharose by modulation of alcohol concentrations. J. Chromatogr., B 2003, 791, 263-272.
24. Tseng, W.-C.; Ho, F.-L.; Fang, T.-Y.; Suen, S.-Y. Effect of alcohol on purification of plasmid DNA using ion-exchange membrane. J. Membr. Sci. 2004, 233, 161-167.
25. Queiroz, J. A.; Tomaz, C. T.; Cabral, J. M. S. Hydrophobic interaction chromatography of proteins. J. Biotechnol. 2001, 87, 143-159.