The use of DRAQ5 to monitor intracellular DNA in escherichia coli by flow cytometry

Journal of Fluorescence

Volumn 20, Issue 4, 2010, Pages 907-914

  Silva, Filomena ^a   Lourenço, Olga ^a   Pina Vaz, Cidália ^b   Rodrigues, Acácio G ^b   Queiroz, João A ^a   Domingues, Fernanda Conceição ^a  

^a UNIVERSITY OF BEIRA INTERIOR   (Portugal)

^b University of Porto   (Portugal)

Author keywords

DNA content; DRAQ5; Escherichia coli; Flow cytometry

Indexed keywords

CELL CYCLE; DNA CONTENT; DRAQ5; E. COLI; EUKARYOTIC CELLS; FLOW-CYTOMETRIC ANALYSIS; GLUTARALDEHYDES; GRAM-NEGATIVE BACTERIA; HIGH-CONTENT; LOW DETECTION LIMIT; MULTIPARAMETER ANALYSIS; PROKARYOTIC CELLS;

ALDEHYDES; BACTERIA; ESCHERICHIA COLI; ETHANOL; FLOW CYTOMETRY; FLUORESCENCE; GENES;

DNA;

1,5 BIS((2 (METHYLAMINO)ETHYL)AMINO) 4,8 DIHYDROXYANTHRACENE 9,10 DIONE; 1,5-BIS((2-(METHYLAMINO)ETHYL)AMINO)-4,8-DIHYDROXYANTHRACENE-9,10-DIONE; ALCOHOL; ANTHRAQUINONE DERIVATIVE; BACTERIAL DNA; FLUORESCENT DYE;

ARTICLE; CELL CYCLE; CYTOLOGY; ENZYME SPECIFICITY; ESCHERICHIA COLI; FLOW CYTOMETRY; INTRACELLULAR SPACE; METABOLISM; METHODOLOGY;

ANTHRAQUINONES; CELL CYCLE; DNA, BACTERIAL; ESCHERICHIA COLI; ETHANOL; FLOW CYTOMETRY; FLUORESCENT DYES; INTRACELLULAR SPACE; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; EUKARYOTA; NEGIBACTERIA; PROKARYOTA;

EID: 77955656727     PISSN: 10530509     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10895-010-0636-y     Document Type: Article

References (30)

1. HM Davey 2002 Flow cytometric techniques for the detection of microorganisms Methods Cell Sci 24 91 97 10.1023/A:1024106317540 1:CAS:528:DC%2BD3sXksFygsb4%3D 12815297 (Pubitemid 36826792)
2. Zahavy E, Heleg-Shabtai V, Zafrani Y, Marciano D, Yitzhaki S (2009) Application of fluorescent nanocrystals (q-dots) for the detection of pathogenic bacteria by flow-cytometry. J Fluoresc (in press)
3. J Roostalu A Joers H Luidalepp N Kaldalu T Tenson 2008 Cell division in Escherichia coli cultures monitored at single cell resolution BMC Microbiol 8 68 81 10.1186/1471-2180-8-68 18430255 (Pubitemid 351678119)
4. N Borth R Mitterbauer D Mattanovich W Kramer K Bayer H Katinger 1998 Flow cytometric analysis of bacterial physiology during induction of foreign protein synthesis in recombinant Escherichia coli cells Cytometry 31 125 129 10.1002/(SICI)1097-0320(19980201)31:2<125::AID-CYTO7>3.0.CO;2-L 1:CAS:528:DyaK1cXhtVCkt7c%3D 9482281 (Pubitemid 28092060)
5. CJ Hewitt H Onyeaka G Lewis IW Taylor AW Nienow 2007 A comparison of high cell density fed-batch fermentations involving both induced and non-induced recombinant Escherichia coli under well-mixed small-scale and simulated poorly mixed large-scale conditions Biotechnol Bioeng 96 495 505 10.1002/bit.21127 1:CAS:528:DC%2BD2sXhtVajtLo%3D 16902956 (Pubitemid 46232355)
6. HM Davey DB Kell 1996 Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses Microbiol Rev 60 641 696 1:CAS:528:DyaK2sXjtF2qug%3D%3D 8987359
7. MK Winson HM Davey 2000 Flow cytometric analysis of microorganisms Methods 21 231 240 10.1006/meth.2000.1003 1:CAS:528:DC%2BD3cXktlGjsrc%3D 10873477 (Pubitemid 32896747)
8. M Canovas V Garcia V Bernal T Torroglosa JL Iborra 2007 Analysis of Escherichia coli cell state by flow cytometry during whole cell catalyzed biotransformation for L-carnitine production Process Biochem 42 25 33 10.1016/j.procbio.2006.07.027 1:CAS:528:DC%2BD28Xht1agsbbF (Pubitemid 44738656)
9. BL Roth M Poot ST Yue PJ Millard 1997 Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain Appl Environ Microbiol 63 2421 2431 1:CAS:528:DyaK2sXjsFGisbk%3D 9172364
10. F Garcia-Ochoa VE Santos A Alcon 1998 Intracellular compounds quantification by means of flow cytometry in bacteria: application to xanthan production by Xanthomonas campestris Biotechnol Bioeng 57 87 94 10.1002/(SICI)1097-0290(19980105)57:1<87::AID-BIT11>3.0.CO;2-5 1:CAS:528:DyaK2sXnvVGnsLc%3D 10099182 (Pubitemid 27514704)
11. E Soriano N Borth H Katinger D Mattanovich 1999 Flow cytometric analysis of metabolic stress effects due to recombinant plasmids and proteins in Escherichia coli production strains Metab Eng 1 270 274 10.1006/mben.1999.0128 1:STN:280:DC%2BD3cvhvFGrsw%3D%3D 10937942 (Pubitemid 129499384)
12. S Velamakanni Y Yao DA Gutmann HW van Veen 2008 Multidrug transport by the ABC transporter Sav1866 from Staphylococcus aureus Biochemistry 47 9300 9308 10.1021/bi8006737 1:CAS:528:DC%2BD1cXps1Knurg%3D 18690712
13. PJ Smith N Blunt M Wiltshire T Hoy P Teesdale-Spittle MR Craven JV Watson WB Amos RJ Errington LH Patterson 2000 Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy Cytometry 40 280 291 10.1002/1097-0320(20000801)40:4<280::AID-CYTO4>3.0.CO;2-7 1:CAS:528:DC%2BD3cXlvFahtL4%3D 10918279 (Pubitemid 30643187)
14. RW Allan MA Ansari-Lari S Jordan 2008 DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry Am J Clin Pathol 129 706 713 10.1309/D2M6Q7BYHWAM1FYQ 18426729
15. Smith PJ, Wiltshire M, Errington RJ (2004) DRAQ5 labeling of nuclear DNA in live and fixed cells. Curr. Protoc. Cytom. Chapter 7, Unit 7.25
16. CM Yuan VK Douglas-Nikitin KP Ahrens GR Luchetta RC Braylan L Yang 2004 DRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties Cytometry B Clin Cytom 58 47 52 10.1002/cyto.b.20000 14994375
17. F Silva L Passarinha F Sousa JA Queiroz FC Domingues 2009 Influence of growth conditions on plasmid DNA production J Microbiol Biotechnol 19 1408 1414 1:CAS:528:DC%2BC3cXlvVWmuw%3D%3D 19996695
18. L Sun BA Jacobson BS Dien F Srienc JA Fuchs 1994 Cell cycle regulation of the Escherichia coli nrd operon: requirement for a cis-acting upstream AT-rich sequence J Bacteriol 176 2415 2426 1:CAS:528:DyaK2cXktVansLY%3D 8157611
19. R Edward 2009 Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells Mol Cells 27 391 396 10.1007/s10059-009-0066-3 1:CAS:528:DC%2BD1MXkvFChtro%3D 19390818
20. KL Njoh LH Patterson M Zloh M Wiltshire J Fisher S Chappell S Ameer-Beg Y Bai D Matthews RJ Errington PJ Smith 2006 Spectral analysis of the DNA targeting bisalkylaminoanthraquinone DRAQ5 in intact living cells Cytometry A 69 805 814 16969814
21. K Swerts N Van Roy Y Benoit G Laureys J Philippe 2007 DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies Clin Chim Acta 379 154 157 10.1016/j.cca.2006.12.008 1:CAS:528:DC%2BD2sXislKitrY%3D 17229416 (Pubitemid 46366966)
22. M Herrero C Quiros LA Garcia M Diaz 2006 Use of flow cytometry to follow the physiological states of microorganisms in cider fermentation processes Appl Environ Microbiol 72 6725 6733 10.1128/AEM.01183-06 1:CAS:528:DC%2BD28XhtFalsLjK 17021224 (Pubitemid 44582915)
23. C Quiros M Herrero LA Garcia M Diaz 2007 Application of flow cytometry to segregated kinetic modeling based on the physiological states of microorganisms Appl Environ Microbiol 73 3993 4000 10.1128/AEM.00171-07 1:CAS:528: DC%2BD2sXntVOgt7g%3D 17483273 (Pubitemid 46983246)
24. M Berney F Hammes F Bosshard HU Weilenmann T Egli 2007 Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry Appl Environ Microbiol 73 3283 3290 10.1128/AEM.02750-06 1:CAS:528:DC%2BD2sXlslSnu7o%3D 17384309 (Pubitemid 46834980)
25. H Zhao F Traganos J Dobrucki D Wlodkowic Z Darzynkiewicz 2009 Induction of DNA damage response by the supravital probes of nucleic acids Cytometry A 75 510 519 19373929
26. P Monfort B Baleux 1996 Cell cycle characteristics and changes in membrane potential during growth of Escherichia coli as determined by a cyanine fluorescent dye and flow cytometry J Microbiol Methods 25 79 86 10.1016/0167-7012(95)00089-5 (Pubitemid 26160248)
27. RM Martin H Leonhardt MC Cardoso 2005 DNA labeling in living cells Cytometry A 67 45 52 16082711
28. S Muller 2007 Modes of cytometric bacterial DNA pattern: a tool for pursuing growth Cell Prolif 40 621 639 10.1111/j.1365-2184.2007.00465.x 1:STN:280:DC%2BD2srlvV2rtQ%3D%3D 17877606 (Pubitemid 47416555)
29. O Michelsen MJ Teixeira de Mattos PR Jensen FG Hansen 2003 Precise determinations of C and D periods by flow cytometry in Escherichia coli K-12 and B/r Microbiology 149 1001 1010 10.1099/mic.0.26058-0 1:CAS:528: DC%2BD3sXjtlyqtLo%3D 12686642 (Pubitemid 36553369)
30. HE Kubitschek ML Freedman 1971 Chromosome replication and the division cycle of Escherichia coli B/r J Bacteriol 107 95 99 1:CAS:528:DyaE3MXks1Sht78%3D 4935333