Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography

Journal of Gene Medicine

Volumn 11, Issue 1, 2009, Pages 79-88

  Sousa, Fani ^a   Prazeres, Duarte M F ^b   Queiroz, João A ^a  

^a UNIVERSITY OF BEIRA INTERIOR   (Portugal)

^b INSTITUTO SUPERIOR TÉCNICO   (Portugal)

Author keywords

Arginine affinity chromatography; Nonviral gene vectors; Supercoiled plasmid; Transfection efficiency

Indexed keywords

ARGININE; PLASMID DNA; ENDOTOXIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CELL STRAIN COS7; CONTROLLED STUDY; DNA PURIFICATION; DNA SUPERCOILING; DRUG CONTAMINATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE TRANSFER; GENETIC TRANSFECTION; MOLECULAR RECOGNITION; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PROTEIN CONTENT; RNA GENE; SEPARATION TECHNIQUE; ANIMAL; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PLASMID;

ESCHERICHIA COLI;

ANIMALS; ARGININE; CERCOPITHECUS AETHIOPS; CHROMATOGRAPHY, AFFINITY; COS CELLS; DNA, SUPERHELICAL; ENDOTOXINS; ESCHERICHIA COLI; PLASMIDS; TRANSFECTION;

EID: 59449090459     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.1272     Document Type: Article

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