A simple in vivo assay for increased protein solubility

Protein Science

Volumn 8, Issue 9, 1999, Pages 1908-1911

  Maxwell, Karen L ^a   Mittermaier, Anthony K ^a,b   Forman Kay, Julie D ^a,b   Davidson, Alan R ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Chloramphenicol acetyltransferase; HIV integrase; In vivo selection; Protein solubility

Indexed keywords

CHLORAMPHENICOL; CHLORAMPHENICOL ACETYLTRANSFERASE; HYBRID PROTEIN; PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; IN VIVO STUDY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; SOLUBILITY;

ESCHERICHIA COLI; FELIS CATUS;

EID: 0032824503     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.9.1908     Document Type: Article

References (16)

1. Dale GE, Broger C, Langen H, D'Arcy A, Stuber D. 1994. Improving protein solubility through rationally designed amino acid replacements: Solubilization of the trimethoprim-resistant type S1 dihydrofolate reductase. Protein Eng 7:933-939.
2. Dyda F, Hickman AB, Jenkins TM, Engelman A, Craigie R, Davies DR. 1994. Crystal structure of the catalytic domain of HIV-1 integrase: Similarity to other polynucleotidyl transferases. Science 266:1981-1986.
3. Hopp TP, Prickett KS, Price VL, Cerretti DP, Urdal DL, Conlon PJ. 1988. BioTechnology 6:1204-1210.
4. Jenkins TM, Hickman AB, Dyda F, Ghirlando R, Davies DR, Craigie R. 1995. Catalytic domain of human immunodeficiency virus type 1 integrase: Identification of a soluble mutant by systematic replacement of hydrophobic residues. Proc Natl Acad Sci USA 92:6057-6061.
5. Ko YH, Thomas PJ, Delannoy MR, Pedersen PL. 1993. The cystic fibrosis transmembrane conductance regulator. Overexpression, purification, and characterization of wild-type and delta F508 mutant forms of the first nucleotide-binding fold in fusion with the maltose-binding protein. J Biol Chem 268:24330-24338.
6. Leistler B, Perham RN. 1994. Solubilizing buried domains of proteins: A self-assembling interface domain from glutathione reductase. Biochemistry 33:2773-2781.
7. Murby M, Samuelsson E, Nguyen TN, Mignard L, Power U, Binz H, Uhlen M, Stahl S. 1995. Hydrophobicity engineering to increase solubility and stability of a recombinant protein from respiratory syncytial virus. Eur J Biochem 230:38-44.
8. Nieba L, Honegger A, Krebber C, Pluckthun A. 1997. Disrupting the hydrophobic patches at the antibody variable/constant domain interface: Improved in vivo folding and physical characterization of an engineered scFv fragment. Protein Eng 10:435-444.
9. Pan H, Clary D, Sadowski PD. 1991. Identification of the DNA-binding domain of the FLP recombinase. J Biol Chem 266:11347-11354.
10. Riordan JR, Rommens JM, Kerem B, Alon N, Rozmahel R, Grzelczak Z, Zielenski J, Lok S, Plavsic N, Chou JL. 1989. Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science 245:1066-10673.
11. Robben J, Massie G, Bosmans E, Wellens B, Volckaert G. 1993. An Escherichia coli plasmid vector system for high-level production and purification of heterologous peptides fused to active chloramphenicol acetyltransferase. Gene 126:109-113.
12. Rose RE. 1988. The nucleotide sequence of pACYC184. Nucleic Acids Res 16:355.
13. Schein CH. 1990. Solubility as a function of protein structure and solvent components. Biotechnology 8:308-317.
14. Sudol M, Greulich H, Newman L, Sarkar A, Sukegawa J, Yamamoto T. 1993. A novel Yes-related kinase, Yrk, is expressed at elevated levels in neural and hematopoietic tissues. Oncogene 8:823-831.
15. Waugh DS, Sauer RT. 1993. Single amino acid substitutions uncouple the DNA-binding and strand scission activities of Fok I endonuclease. Proc Natl Acad Sci USA 90:9596-9600.
16. Yanisch-Perron C, Vieira J, Messing J. 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.