Display of active subtilisin 309 on phage: Analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors

Journal of Molecular Biology

Volumn 296, Issue 1, 2000, Pages 87-102

  Legendre, Daniel ^a   Laraki, Nezha ^a   Gräslund, Torbjörn ^b   Bjørnvad, Mads E ^c   Bouchet, Michèle ^a   Nygren, Per Åke ^b   Borchert, Torben V ^c   Fastrez, Jacques ^a  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^c NOVO NORDISK A S   (Denmark)

Author keywords

Biotinylated inhibitors; Enzyme selection; Phage display; Specificity; Subtilisin

Indexed keywords

DETERGENT; ENZYME INHIBITOR; ESTER DERIVATIVE; LYSINE; STREPTAVIDIN; SUBTILISIN; TETRAPEPTIDE;

ARTICLE; BIOTINYLATION; CONTROLLED STUDY; EGGERTHELLA LENTA; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME RELEASE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BACILLUS LENTUS; EGGERTHELLA LENTA;

EID: 0034635339     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3437     Document Type: Article

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