Covalent immobilization of tobacco-etch-virus NIa protease: A useful tool for cleavage of the histidine tag of recombinant proteins

Biotechnology and Applied Biochemistry

Volumn 53, Issue 3, 2009, Pages 165-174

  Puhl, Ana Cristina ^a   Giacomini, Cecilia ^b   Irazoqui, Gabriela ^b   Batista Viera, Francisco ^b   Villarino, Andrea ^a,c   Terenzi, Hernán ^a  

^a FEDERAL UNIVERSITY OF SANTA CATARINA   (Brazil)

^b UNIVERSIDAD DE LA REPÚBLICA   (Uruguay)

^c INSTITUT PASTEUR DE MONTEVIDEO   (Uruguay)

Author keywords

Covalent immobilization; Histidine tag; Recombinant protein; Thiolsulfinate agarose; Tobacco etch virus NIa protease (TEV protease)

Indexed keywords

AMINO ACIDS; ENZYME ACTIVITY; TOBACCO; VIRUSES;

AFFINITY PURIFICATION; AGAROSE; CONTINUOUS PRODUCTION; COVALENT IMMOBILIZATION; HISTIDINE TAG; LOSS OF PERFORMANCE; PROTEIN IMMOBILIZATION; TEV PROTEASE;

RECOMBINANT PROTEINS;

AMINO ACID; CYSTEINE PROTEINASE; CYSTINE; LYSINE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN; TEV PROTEASE; THIOL GROUP; TOBACCO ETCH VIRUS NIA PROTEASE; UNCLASSIFIED DRUG; GLUTARALDEHYDE; HISTIDINE; HYBRID PROTEIN; IMMOBILIZED ENZYME; OLIGOPEPTIDE; PROTEINASE; SEPHAROSE; VIRUS PROTEIN;

ARTICLE; CONTROLLED STUDY; COVALENT BOND; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME SYNTHESIS; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN PURIFICATION; CHEMISTRY; ENZYMOLOGY; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; POTYVIRUS;

NIA; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

ENDOPEPTIDASES; ENZYME STABILITY; ENZYMES, IMMOBILIZED; GLUTARAL; HISTIDINE; KINETICS; OLIGOPEPTIDES; POTYVIRUS; RECOMBINANT FUSION PROTEINS; SEPHAROSE; VIRAL PROTEINS;

EID: 67651028031     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20080063     Document Type: Article

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