Engineering soluble proteins for structural genomics

Nature Biotechnology

Volumn 20, Issue 9, 2002, Pages 927-932

  Pédelacq, Jean Denis ^a   Piltch, Emily ^b   Liong, Elaine C ^a   Berendzen, Joel ^a   Kim, Chang Yub ^a   Rho, Beom Seop ^a   Park, Min S ^a   Terwilliger, Thomas C ^a   Waldo, Geoffrey S ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF ROCHESTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; CRYSTALLIZATION; ESCHERICHIA COLI; GENES; SOLUBILITY; X RAY CRYSTALLOGRAPHY;

NATURAL HOMOLOGS;

PROTEINS;

GREEN FLUORESCENT PROTEIN; HYPERTHERMOPHILIC PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; GENE STRUCTURE; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN VARIANT; SOLUBILIZATION; STRUCTURE ANALYSIS; THERMAL ANALYSIS; THREE DIMENSIONAL IMAGING; X RAY CRYSTALLOGRAPHY;

EVOLUTION, MOLECULAR; GENOME, BACTERIAL; GENOMICS; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MODELS, MOLECULAR; NUCLEOSIDE-DIPHOSPHATE KINASE; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY; SUBSTRATE SPECIFICITY; THERMOPROTEACEAE;

ESCHERICHIA COLI;

EID: 0036713170     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt732     Document Type: Article

References (33)

1. Structural proteomics: Prospects for high throughput sample preparation
2. Archaeal genomics
3. Strategies for achieving high-level expression of genes in Escherichia coli
4. A new protein folding screen: Application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase
5. In vitro folding of inclusion body proteins
6. Rapid protein-folding assay using green fluorescent protein
7. Polymerization of the SAM domain of TEL in leukemogenesis and transcriptional repression
8. Potential use of additivity of mutational effects in simplifying protein engineering
9. Folding and function of a T4 lysozyme containing 10 consecutive alanines illustrate the redundancy of information in an amino acid sequence
10. Immobilized metal ion affinity chromatography
11. Properties; in theory and practice; of novel gel-filtration media for standard liquid-chromatography
12. Three-dimensional structure of nucleoside diphosphate kinase
13. Rapid evolution of a protein in vitro by DNA shuffling
14. Nm23/nucleoside diphosphate kinase: Toward a structural and biochemical understanding of its biological functions
15. The catalytic mechanism of nucleoside diphosphate kinases
16. Protein structure comparison by alignment of distance matrices
17. X-ray structure of human nucleoside diphosphate kinase B complexed with GDP at 2 Å resolution
18. Real-time bioluminometric method for detection of nucleoside diphosphate kinase activity
19. X-ray structure of nucleoside diphosphate kinase complexed with thymidine diphosphate and Mg2+ at 2 Å resolution
20. Improving protein solubility through rationally designed amino acid replacements: Solubilization of the trimethoprim-resistant type S 1 dihydrofolate reductase
21. Crystal structure of Myxococcus xanthus nucleoside diphosphate kinase and its interaction with a nucleotide substrate at 2 Å resolution
22. Quaternary structure of nucleoside diphosphate kinases
23. The human Nm23/nucleoside diphosphate kinases
24. Crystallization and preliminary X-ray crystallographic analysis of the Rv2002 gene product from Mycobacterium tuberculosis, a β-ketoacyl carrier protein reductase homologue
25. Detection and assay of proteases using calf lens β-crystallin aggregate as substrate
26. Atomic structures of the human immunophilin FKBP-12 complexes with FK506 and rapamycin
27. The CCP4 suite: Programs for protein crystallography
28. Automated MAD MIR structure solution
29. Reciprocal-space solvent flattening
30. Crystallography & NMR system: A new software suite for macromolecular structure determination
31. Molscript: A program to produce both detailed and schematic plots of protein structures
32. Raster3D: Photorealistic molecular graphics
33. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons