The mechanisms of cytotoxicity of urethane dimethacrylate to Chinese hamster ovary cells

Biomaterials

Volumn 31, Issue 27, 2010, Pages 6917-6925

  Chang, Hsiao Hua ^a   Chang, Mei Chi ^b   Lin, Li Deh ^a   Lee, Jang Jaer ^a   Wang, Tong Mei ^a   Huang, Chun Hsun ^b   Yang, Ting Ting ^c   Lin, Hsueh Jen ^d   Jeng, Jiiang Huei ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b CHANG GUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

^c Hsin Chu General Hospital   (Taiwan)

^d SHOW CHWAN MEMORIAL HOSPITAL   (Taiwan)

Author keywords

Apoptosis; Cell cycle; Cytotoxicity; Glutathione; Reactive oxygen species; Urethane dimethacrylate

Indexed keywords

APOPTOSIS; CELL CYCLE; GLUTATHIONES; REACTIVE OXYGEN SPECIES; URETHANE DIMETHACRYLATE;

CYTOTOXICITY; DENTAL MATERIALS; MONOMERS; OXYGEN; RESINS;

CELL DEATH;

ACETYLCYSTEINE; CATALASE; GLUTATHIONE; LIPOCORTIN 5; REACTIVE OXYGEN METABOLITE; TRYPAN BLUE; UNCLASSIFIED DRUG; URETHAN DERIVATIVE; URETHANE DIMETHACRYLATE; METHACRYLIC ACID DERIVATIVE; POLYURETHAN; URETHANE DIMETHACRYLATE LUTING RESIN;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CELL GROWTH; CHO CELL; CYTOTOXICITY; DRUG EFFECT; GROWTH INHIBITION; NECROSIS; NONHUMAN; PRIORITY JOURNAL; ANIMAL; CELL CYCLE; CHO CELL LINE; CRICETULUS; DRUG EFFECTS; HAMSTER; METABOLISM;

ANIMALS; APOPTOSIS; CATALASE; CELL CYCLE; CHO CELLS; CRICETINAE; CRICETULUS; GLUTATHIONE; METHACRYLATES; POLYURETHANES; REACTIVE OXYGEN SPECIES;

CRICETULUS GRISEUS;

EID: 77954382829     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2010.05.059     Document Type: Article

References (56)

1. Bakopoulou A., Papadopoulos T., Garefis P. Molecular toxicology of substances released from resin-based dental restorative materials. Int J Mol Sci 2009, 10:3861-3899.
2. Rueggeberg F.A. From vulcanite to vinyl, a history of resins in restorative dentistry. J Prosthet Dent 2002, 87:364-379.
3. Yesilyurt C., Yoldas O., Altintas S.H., Kusgoz A. Effects of food-simulating liquids on the mechanical propertoes of a silorane-based dental composite. Dent Mater J 2009, 28:362-367.
4. Bagls Y.H., Baltacloglu I.H., Kahyaogullari S. Comparing microleakage and the layering methods of silorane-based resin composite in wide Class II MOD cavities. Oper Dent 2009, 34:578-585.
5. Hume W.R., Gerzina T.M. Bioavailability of components of resin-based materials which are applied to teeth. Crit Rev Oral Biol Med 1996, 7:172-179.
6. Barszczewska-Rybarek I.M. Structure-property relationships in dimethacrylate networks based on Bis-GMA, UDMA and TEGDMA. Dent Mater 2009, 25:1082-1089.
7. Polydorou O., Konig A., Hellwig E., Kummerer K. Urethane dimethacrylate: a molecule that may cause confusion in dental research. J Biomed Mater Res B Appl Biomater 2009, 91:1-4.
8. Spahl W., Budzikiewicz H., Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrometry. J Dent 1998, 26:137-145.
9. Sideridou I.D., Achilias D.S. Elution study of unreacted Bis-GMA, TEGDMA, UDMA and Bis-EMA from light-cured dental resins and resin composites using HPLC. J Biomed Mater Res Part B Appl Biomater 2005, 74(B):617-626.
10. Geurtsen W. Biocompatibility of resin-modified filling materials. Crit Rev Oral Biol Med 2000, 11:333-355.
11. Hamid A., Hume W.R. The effect of dentin thickness on diffusion of resin monomers in vitro. J Oral Rehabit 1997, 24:20-25.
12. Ovist V., Stoltze K., Ovist J. Human pulp reactions to resin restorations performed with different acid etch restorative procedures. Acta Odontol Scand 1989, 47:253-267.
13. Fuks A.B., Cleaton-Jones P. Pulp response to a composite resin inserted in deep cavities with and without surface seal. J Prosthet Dent 1990, 63:129-134.
14. Urcan E., Haertel U., Styllou M., Hickel R., Scherthan H., Reichl F.X. Real-time xCELLigence impedance analysis of the cytotoxicity of dental composite components on human gingival fibroblasts. Dent Mater 2010, 26:51-58.
15. Reichl F.X., Simon S., Esters M., Seiss M., Kehe K., Kleinsasser N., et al. Cytotoxicity of dental composite (co)monomers and the amalgam component Hg(2+) in human gingival fibroblasts. Arch Toxicol 2006, 80:465-472.
16. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res 1997, 37:517-524.
17. Schwengberg S., Bohlen H., Kleinsasser N., Kehe K., Seiss M., Walther U.I., et al. In vitro embryotoxicity assessment with dental restorative materials. J Dent 2005, 33:49-55.
18. About I., Camps J., Mitsiadis T.A., Bottero M.J., Butler W., Franquin J.C. Influence of resinous monomers on the differentiation in vitro of human pulp cells into odontoblasts. J Biomed Mater Res 2002, 63:418-423.
19. Nocca G., Martorana G.E., De Sole P., De Palma F., Calla C., Corsale P., et al. Effects of 1,4-butanediol dimethacrylate and urethane dimethacrylate on HL-60 cell metabolism. Eur J Oral Sci 2009, 117:175-181.
20. Rakich D.R., Wataha J.C., Lefebvre C.A., Weller R.N. Effect of dentin bonding agents on the secretion of inflammatory mediators from macrophages. J Endod 1999, 25:114-117.
21. Schweikl H., Schmalz G., Spruss T. The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res 2001, 80:1615-1620.
22. Schweikl H., Schmalz G., Rackebrandt K. The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res 1998, 415:119-130.
23. Heil J., Reifferscheid G., Waldmann P., Leyhausen G., Geurtsen W. Genotoxicity of dental materials. Mutat Res 1996, 368:181-194.
24. Kleinsasser N.H., Schmid K., Sassen A.W., Harreus U.A., Staudenmaier R., Folwaczny M., et al. Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay. Biomaterials 2006, 27:1762-1770.
25. Chang M.C., Lin L.D., Chan C.P., Chang H.H., Chen L.I., Lin H.J., et al. The effect of BisGMA on cyclooxygenase-2 expression, PGE2 production and cytotoxicity via reactive oxygen species- and MEK/ERK-dependent and -independent pathways. Biomaterials 2009, 30:4070-4077.
26. Chang H.H., Guo M.K., Kasten F.H., Chang M.C., Huang G.F., Wang Y.L., et al. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 2005, 26:745-753.
27. Schweikl H., Spagnuolo G., Schmalz G. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006, 85:870-877.
28. Schweikl H., Hartmann A., Hiller K.A., Spagnuolo G., Bolay C., Brockhoff G., et al. Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater 2007, 23:688-695.
29. Spagnuolo G., D'Anto V., Cosentino C., Schmalz G., Schweikl H., Rengo S. Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. Biomaterials 2006, 27:1803-1809.
30. Calabrese E.J., Canada A.T. Catalase: its role in xenobiotic detoxification. Pharmacol Ther 1989, 44:297-307.
31. Yeh H.W., Chang M.C., Lin C.P., Tseng W.Y., Chang H.H., Wang T.M., et al. Comparative cytotoxicity of five current dentin bonding agents: role of cell cycle deregulation. Acta Biomater 2009, 5:3404-3410.
32. Lin C.C., Chang M.C., Chang H.H., Wang T.M., Tseng W.Y., Tai T.F., et al. Areca nut-induced micronuclei and cytokinesis failure in Chinese hamster ovary cells is related to reactive oxygen species production and actin filament deregulation. Environ Mol Mutagen 2009, 50:367-374.
33. Seow H.A., Perketh P.G., Belcourt M.F., Tomasz M., Rockwell S., Sarterelli A.C. Nuclear overexpression of NAD(P)H:quinone oxidoreductase 1 in Chinese hamster ovary cells increases the cytotoxicity of mitomycin C under aerobic and hypoxic condictions. J Biol Chem 2004, 279:31606-31612.
34. Crea F., Sarti D., Falciani F., Al-Rubeal M. Over-expression of hTERT in CHO K1 results in decreased apoptosis and reduced serum dependency. J Biotechnol 2006, 121:109-123.
35. Jeng J.H., Hahn L.J., Lu F.J., Wang Y.J., Kuo M.Y. Eugenol triggers different pathobiological effects on human oral mucosal fibroblasts. J Dent Res 1994, 73:1050-1055.
36. Chang M.C., Chan C.P., Wang Y.J., Lee P.H., Chen L.I., Tsai Y.L., et al. Induction of necrosis and apoptosis to KB cancer cells by sanguinarine is associated with reactive oxygen species production and mitochondrial membrane depolarization. Toxicol Appl Pharmacol 2007, 218:143-151.
37. Lee P.H., Chang M.C., Chang W.H., Wang T.M., Wang Y.J., Hahn L.J., et al. Prolonged exposure to arecoline arrested human KB epithelial cell growth: regulatory mechanisms of cell cycle and apoptosis. Toxicology 2006, 220:81-89.
38. Gomes A., Fernandes E., Lima J.L.F.C. Fluorescence probes used for detection of reactive oxygen species. J Biochem Biophys Methods 2005, 65:45-80.
39. Eastman A., Rigas J.R. Modulation of apoptosis signaling pathways and cell cycle regulation. Semin Oncol 1999, 26:7-16.
40. Reichl F.X., Esters M., Simon S., Seiss M., Kehe K., Kleinsasser N., et al. Cell death effects of resin-based dental material compounds and mercurials in human gingival fibroblasts. Arch Toxicol 2006, 80:370-377.
41. Shinzawa K., Tsujimoto Y. PLA2 activity is required for nuclear shrinkage in caspase-independent cell death. J Cell Biol 2003, 163:1219-1230.
42. Geurtsen W., Lehmann F., Spahl W., Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res 1998, 41:474-480.
43. Nassiri M.R., Hanks C.T., Cameron M.J., Strawn S.E., Craig R.G. Application of flow cytometry to determine the cytotoxicity of urethane dimethacrylate in human cells. J Biomed Mater Res 1994, 28:153-158.
44. Lukas J., Lukas C., Bartek J. Mammalian cell cycle checkpoints: signalling pathways and their organization in space and time. DNA Repair (Amst) 2004, 3:997-1007.
45. Valko M., Leibfritz D., Moncol J., Cronin M.T., Mazur M., Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007, 39:44-84.
46. Stanislawski L., Lefeuvre M., Bourd K., Soheili-Majd E., Goldberg M., Perianin A. TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 2003, 66:476-482.
47. Reichl F.X., Seiss M., Marquardt W., Kleinsasser N., Schweikl H., Kehe K., et al. Toxicity potentiation by H2O2 with components of dental restorative materials on human oral cells. Arch Toxicol 2008, 82:21-28.
48. Nocca G., D'Anto V., Desiderio C., Rossetti D.V., Valletta R., Baguala A.M., et al. N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation. Biomaterials 2010, 31:2508-2516.
49. Paranjpe A., Cacalano N.A., Hume W.R., Jewett A. N-acetylcysteine protects dental pulp stromal cells from HEMA-induced apoptosis by inducing differentiation of the cells. Free Radic Biol Med 2007, 43:1394-1408.
50. Jiang Z.Q., Chen C., Yang B., Hebbar V., Kong A.N. Differential responses from seven mammalian cell lines to the treatments of detoxifying enzyme inducers. Life Sci 2003, 72:2243-2253.
51. Duthie S.J., Collins A.R. The influence of cell growth, detoxifying enzymes and DNA repair on hydrogen peroxide-mediated DNA damage (measured using the comet assay) in human cells. Free Radic Biol Med 1997, 22:717-724.
52. Poot M., Kavanagh T.J., Kang H.C., Haugland R.P., Rabinovitch P.S. Flow cytometric analysis of cell cycle-dependent changes in cell thiol level by combining a new laser dye with Hoechst 333421. Cytometry 1991, 12:184-187.
53. Sarkar A., Mandal G., Singl N., Sundar S., Chatterjee M. Flow cytometric determination of intracellular non-protein thiols in Leishmania promastigotes using 5-chloromethyl fluorescein diacetate. Exp Parasitol 2009, 122:299-305.
54. Chang M.C., Ho Y.S., Lee P.H., Chan C.P., Lee J.J., Hahn L.J., et al. Areca nut extract and arecoline induced the cell cycle arrest but not apoptosis of cultured oral KB epithelial cells: association of glutathione, reactive oxygen species and mitochondrial membrane potential. Carcinogenesis 2001, 22:1527-1535.
55. Chang M.C., Uang B.J., Wu H.L., Lee J.J., Hahn L.J., Jeng J.H. Inducing the cell cycle arrest and apoptosis of oral KB carcinoma cells by hydroxychavicol: roles of glutathione and reactive oxygen species. Br J Pharmacol 2002, 135:619-630.
56. Engelmann J., Janke V., Volk J., Leyhausen G., von Neuhoff N., Schlegelberger B., et al. Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro. Biomaterials 2004, 25:4573-4580.