Micronucleus and comet assay in the peripheral blood of juvenile rat: Establishment of assay feasibility, time of sampling and the induction of DNA damage

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 700, Issue 1-2, 2010, Pages 86-94

  Mughal, A ^a   Vikram, A ^a   Ramarao, P ^a   Jena, G B ^a  

^a NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH NIPER   (India)

Author keywords

Comet; Correlation; DNA damage; Micronucleus; Peripheral blood

Indexed keywords

CHLORAMBUCIL; CISPLATIN; CYCLOPHOSPHAMIDE; DNA; METHOTREXATE; PACLITAXEL;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BLOOD; COMET ASSAY; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DAMAGE; DOSE TIME EFFECT RELATION; DRUG MECHANISM; ERYTHROCYTE; FEASIBILITY STUDY; GENOTOXICITY; GROUPS BY AGE; MICRONUCLEUS TEST; NONHUMAN; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; RAT; SAMPLING; SPRAGUE DAWLEY RAT;

ANIMALS; COMET ASSAY; DNA DAMAGE; ERYTHROCYTES; FEASIBILITY STUDIES; LYMPHOCYTES; MICRONUCLEUS TESTS; RATS; TIME FACTORS;

RATTUS;

EID: 77953911670     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2010.05.014     Document Type: Article

References (50)

1. Dearfield K.L., Cimino M.C., McCarroll N.E., Mauer I., Valcovic L.R. Genotoxicity risk assessment: a proposed classification strategy. Mutat. Res. 2002, 521:121-135.
2. Jena G.B., Kaul C.L., Ramarao P. Genotoxicity testing, a regulatory requirement for drug discovery and development: impact of ICH guidelines. Ind. J. Pharmacol. 2002, 34:86-99.
3. Vikram A., Ramarao P., Jena G.B. Prior bleeding enhances the sensitivity of peripheral blood and bone marrow micronucleus tests in rats. Mutagenesis 2007, 22:287-291.
4. He J.L., Chen W.L., Jin L.F., Jin H.Y. Comparative evaluation of the in vitro micronucleus test and the comet assay for the detection of genotoxic effects of X-ray radiation. Mutat. Res. 2000, 469:223-231.
5. Krishna G., Hayashi M. In vivo rodent micronucleus assay: protocol, conduct and data interpretation. Mutat. Res. 2000, 455:155-166.
6. Tripathi D.N., Pawar A.A., Vikram A., Ramarao P., Jena G.B. Use of the alkaline comet assay for the detection of transplacental genotoxins in newborn mice. Mutat. Res. 2008, 653:134-139.
7. Vikram A., Tripathi D.N., Pawar A.A., Ramarao P., Jena G.B. Pre-bled-young-rats in genotoxicity testing: a model for peripheral blood micronucleus assay. Regul. Toxicol. Pharmacol. 2008, 52:147-157.
8. Brendler-Schwaab S., Hartmann A., Pfuhler S., Speit G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 2005, 20:245-254.
9. Heuser V.D., de Andrade V.M., Peres A., Gomes de Macedo Braga L.M., Bogo Chies J.A. Influence of age and sex on the spontaneous DNA damage detected by micronucleus test and comet assay in mice peripheral blood cells. Cell Biol. Int. 2008, 32:1223-1229.
10. Tripathi D.N., Jena G.B. Ebselen attenuates cyclophosphamide-induced oxidative stress and DNA damage in mice. Free Radic. Res. 2008, 42:966-977.
11. Tripathi D.N., Jena G.B. Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage: a study in mice. Chem. Biol. Interact. 2009, 180:398-406.
12. Laffon B., Pasaro E., Mendez J. Genotoxic effects of styrene-7,8-oxide in human white blood cells: comet assay in relation to the induction of sister-chromatid exchanges and micronuclei. Mutat. Res. 2001, 491:163-172.
13. Robbiano L., Carrozzino R., Puglia C.P., Corbu C., Brambilla G. Correlation between induction of DNA fragmentation and micronuclei formation in kidney cells from rats and humans and tissue-specific carcinogenic activity. Toxicol. Appl. Pharmacol. 1999, 161:153-159.
14. Van Goethem F., Lison D., Kirsch-Volders M. Comparative evaluation of the in vitro micronucleus test and the alkaline single cell gel electrophoresis assay for the detection of DNA damaging agents: genotoxic effects of cobalt powder, tungsten carbide and cobalt-tungsten carbide. Mutat. Res. 1997, 392:31-43.
15. Franke S.I., Pra D., Erdtmann B., Henriques J.A., da Silva J. Influence of orange juice over the genotoxicity induced by alkylating agents: an in vivo analysis. Mutagenesis 2005, 20:279-283.
16. Suzuki H., Takasawa H., Kobayashi K., Terashima Y., Shimada Y., Ogawa I., Tanaka J., Imamura T., Miyazaki A., Hayashi M. Evaluation of a liver micronucleus assay with 12 chemicals using young rats (II): a study by the Collaborative Study Group for the Micronucleus Test/Japanese Environmental Mutagen Society-Mammalian Mutagenicity Study Group. Mutagenesis 2009, 24:9-16.
17. Tripathi D.N., Jena G.B. Astaxanthin intervention ameliorates cyclophosphamide-induced oxidative stress, DNA damage and early hepatocarcinogenesis in rat: role of Nrf2, p53, p38 and phase-II enzymes. Mutat. Res. 2010, 696:69-80.
18. Witt K.L., Livanos E., Kissling G.E., Torous D.K., Caspary W., Tice R.R., Recio L. Comparison of flow cytometry- and microscopy-based methods for measuring micronucleated reticulocyte frequencies in rodents treated with nongenotoxic and genotoxic chemicals. Mutat. Res. 2008, 649:101-113.
19. Criswell K.A., Krishna G., Zielinski D., Urda G.A., Juneau P., Bulera S., Bleavins M.R. Validation of a flow cytometric acridine orange micronuclei methodology in rats. Mutat. Res. 2003, 528:1-18.
20. Al-Ali S.Y., Hassan I.M., Sadek S. Ultrastructural changes in rat livers perfused in vitro and in vivo with a high dose of methotrexate. Histol. Histopathol. 2005, 20:1131-1145.
21. Madhyastha S., Prabhu L.V., Saralaya V., Rai R. A comparison of vitamin A and leucovorin for the prevention of methotrexate-induced micronuclei production in rat bone marrow. Clinics 2008, 63:821-826.
22. Chirino Y.I., Sanchez-Gonzalez D.J., Martinez-Martinez C.M., Cruz C., Pedraza-Chaverri J. Protective effects of apocynin against cisplatin-induced oxidative stress and nephrotoxicity. Toxicology 2008, 245:18-23.
23. MacGregor J.T., Bishop M.E., McNamee J.P., Hayashi M., Asano N., Wakata A., Nakajima M., Saito J., Aidoo A., Moore M.M., Dertinger S.D. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes. II. An efficient method of monitoring chromosomal damage in the rat. Toxicol. Sci. 2006, 94:92-107.
24. Yoshida M., Iizuka K., Terada A., Hara M., Nishijima H., Shimada A., Nakada K., Satoh Y., Akama Y. Prevention of nephrotoxicity of cisplatin by repeated oral administration of ebselen in rats. Tohoku J. Exp. Med. 2000, 191:209-220.
25. Kadota T., Chikazawa H., Kondoh H., Ishikawa K., Kawano S., Kuroyanagi K., Hattori N., Sakakura K., Koizumi S., Hiraiwa E., et al. Toxicity studies of paclitaxel. (II)-One-month intermittent intravenous toxicity in rats. J. Toxicol. Sci. 1994, 19:11-34.
26. Kadota T., Kondoh H., Chikazawa H., Kuroyanagi K., Hattori N., Ishikawa K., Kawano S., Sakakura K., Koizumi S., Hiraiwa E., et al. Toxicity studies of paclitaxel. (III)-Six-month intermittent intravenous toxicity in rats. J. Toxicol. Sci. 1994, 19:35-55.
27. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 1988, 175:184-191.
28. Giannotti E., Vandin L., Repeto P., Comelli R. A comparison of the in vitro comet assay with the in vitro chromosome aberration assay using whole human blood or Chinese hamster lung cells: validation study using a range of novel pharmaceuticals. Mutagenesis 2002, 17:163-170.
29. Dertinger S.D., Bishop M.E., McNamee J.P., Hayashi M., Suzuki T., Asano N., Nakajima M., Saito J., Moore M., Torous D.K., Macgregor J.T. Flow cytometric analysis of micronuclei in peripheral blood reticulocytes. I. Intra- and interlaboratory comparison with microscopic scoring. Toxicol. Sci. 2006, 94:83-91.
30. Kim I.Y., Hyun C.K. Comparative evaluation of the alkaline comet assay with the micronucleus test for genotoxicity monitoring using aquatic organisms. Ecotoxicol. Environ. Saf. 2006, 64:288-297.
31. Pfuhler S., Kirkland D., Kasper P., Hayashi M., Vanparys P., Carmichael P., Dertinger S., Eastmond D., Elhajouji A., Krul C., Rothfuss A., Schoening G., Smith A., Speit G., Thomas C., van Benthem J., Corvi R. Reduction of use of animals in regulatory genotoxicity testing: identification and implementation opportunities-report from an ECVAM workshop. Mutat. Res. 2009, 680:31-42.
32. Hayashi M., MacGregor J.T., Gatehouse D.G., Blakey D.H., Dertinger S.D., Abramsson-Zetterberg L., Krishna G., Morita T., Russo A., Asano N., Suzuki H., Ohyama W., Gibson D. In vivo erythrocyte micronucleus assay. III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit test. Mutat. Res. 2007, 627:10-30.
33. Suzuki H., Ikeda N., Kobayashi K., Terashima Y., Shimada Y., Suzuki T., Hagiwara T., Hatakeyama S., Nagaoka K., Yoshida J., Saito Y., Tanaka J., Hayashi M. Evaluation of liver and peripheral blood micronucleus assays with 9 chemicals using young rats. A study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/Japanese Environmental Mutagen Society (JEMS)-Mammalian Mutagenicity Study Group (MMS). Mutat. Res. 2005, 583:133-145.
34. Imaoka S., Fujita S., Funae Y. Age-dependent expression of cytochrome P-450s in rat liver. Biochim. Biophys. Acta 1991, 1097:187-192.
35. Tripathi D.N., Jena G.B. Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamide in mice germ cells. Toxicology 2008, 248:96-103.
36. Keshava C., Keshava N., Whong W.Z., Nath J., Ong T.M. Inhibition of methotrexate-induced chromosomal damage by folinic acid in V79 cells. Mutat. Res. 1998, 397:221-228.
37. Gonzalez V.M., Fuertes M.A., Alonso C., Perez J.M. Is cisplatin-induced cell death always produced by apoptosis?. Mol. Pharmacol. 2001, 59:657-663.
38. Wang T.H., Wang H.S., Soong Y.K. Paclitaxel-induced cell death: where the cell cycle and apoptosis come together. Cancer 2000, 88:2619-2628.
39. Higashikuni N., Sutou S. An optimal, generalized sampling time of 30 +/- 6h after double dosing in the mouse peripheral blood micronucleus test. Mutagenesis 1995, 10:313-319.
40. Hirai O., Miyamae Y., Fujino Y., Izumi H., Miyamoto A., Noguchi H. Prior bleeding enhances the sensitivity of the in vivo micronucleus test. Mutat. Res. Lett. 1991, 264:109-114.
41. Poot M., Schuster A., Hoehn H. Cytostatic synergism between bromodeoxyuridine, bleomycin, cisplatin and chlorambucil demonstrated by a sensitive cell kinetic assay. Biochem. Pharmacol. 1991, 41:1903-1909.
42. Branham M.T., Nadin S.B., Vargas-Roig L.M., Ciocca D.R. DNA damage induced by paclitaxel and DNA repair capability of peripheral blood lymphocytes as evaluated by the alkaline comet assay. Mutat. Res. 2004, 560:11-17.
43. Digue L., Orsiere T., De Meo M., Mattei M.G., Depetris D., Duffaud F., Favre R., Botta A. Evaluation of the genotoxic activity of paclitaxel by the in vitro micronucleus test in combination with fluorescent in situ hybridization of a DNA centromeric probe and the alkaline single cell gel electrophoresis technique (comet assay) in human T-lymphocytes. Environ. Mol. Mutagen. 1999, 34:269-278.
44. Lee M., Kwon J., Chung M.K. Enhanced prediction of potential rodent carcinogenicity by utilizing comet assay and apoptotic assay in combination. Mutat. Res. 2003, 541:9-19.
45. Host H. Regeneration of bone marrow cells in rats following cyclophosphamide or total body irradiation. Acta Radiol. Ther. Phys. Biol. 1966, 4:337-352.
46. Hamada S., Yamasaki K.i., Nakanishi S., Omori T., Serikawa T., Hayashi M. Evaluation of the general suitability of the rat for the micronucleus assay: the effect of cyclophosphamide in 14 strains. Mutat. Res. 2001, 495:127-134.
47. Kasahara Y., Wakata A., Nakai Y., Yuno K., Miura D., Yagi K., Hirabayashi K., Makita T. The micronucleus test using peripheral blood reticulocytes from methotrexate-treated mice. Mutat. Res. 1992, 278:145-151.
48. Kumaravel T.S., Vilhar B., Faux S.P., Jha A.N. Comet assay measurements: a perspective. Cell Biol. Toxicol. 2009, 25:53-64.
49. Collins A.R., Oscoz A.A., Brunborg G., Gaivao I., Giovannelli L., Kruszewski M., Smith C.C., Stetina R. The comet assay: topical issues. Mutagenesis 2008, 23:143-151.
50. Burlinson B., Tice R.R., Speit G., Agurell E., Brendler-Schwaab S.Y., Collins A.R., Escobar P., Honma M., Kumaravel T.S., Nakajima M., Sasaki Y.F., Thybaud V., Uno Y., Vasquez M., Hartmann A. Fourth International Workgroup on Genotoxicity testing: results of the in vivo comet assay workgroup. Mutat. Res. 2007, 627:31-35.