DNA damage induced by paclitaxel and DNA repair capability of peripheral blood lymphocytes as evaluated by the alkaline comet assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 560, Issue 1, 2004, Pages 11-17

  Branham, María Teresita ^a   Nadin, Silvina Beatriz ^a   Vargas Roig, Laura María ^a   Ciocca, Daniel Ramón ^a  

^a INSTITUTO ARGENTINO DE NIVOLOGÍA   (Argentina)

Author keywords

Comet assay; DNA damage; DNA repair; Paclitaxel

Indexed keywords

ANTIINFECTIVE AGENT; PACLITAXEL;

ARTICLE; BLOOD SAMPLING; CELL; CELL CULTURE; CELL ISOLATION; COMET ASSAY; CONCENTRATION (PARAMETERS); DNA DAMAGE; DNA REPAIR; HUMAN; INCUBATION TIME; LYMPHOCYTE; NORMAL HUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; VEIN PUNCTURE;

ADULT; ALKALIES; ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELLS, CULTURED; COMET ASSAY; DNA; DNA DAMAGE; FEMALE; HUMANS; LYMPHOCYTES; MALE; MIDDLE AGED; PACLITAXEL;

EID: 11344286813     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2004.01.013     Document Type: Article

References (26)

1. Burkhart C.A., Berman J.W., Swindell C.S., Horowitz S.B. Relationship between the structure of taxol and other taxanes on induction of tumor necrosis factor-α gene expression and cytotoxicity. Cancer Res. 54:1994;5779-5782.
2. Manfredi J.J., Pamess J., Horowitz S.B. Taxol binds to cellular microtubules. J. Cell Biol. 94:1982;688-696.
3. Schiff P.B., Fant J., Horowitz S.B. Promotion of microtubule assembly in vitro by taxol. Nature (Lond.). 277:1979;665-667.
4. Fuchs D.A., Johnson R.K. Cytologic evidence that paclitaxel, an antineoplasic agent from Taxus bevifolia, acts as a mitotic spindle poison. Cancer Treat. Rep. 22:1978;1219-1222.
5. Ollikainen T., Knuuttila A., Suhonen S., Taavitsainen M., Jekunen A., Mattson K., Linnainmaa K. In vitro sensitivity of normal human mesothelial and malignant mesothelioma cell line to four new chemotherapeutic agents. Anti-Cancer Drugs. 11:2000;93-99.
6. Digue L., Orsiere T., De Meo M., Mattei M.G., Depetris D., Duffaud F., Favre R., Boffa A. Evaluation of the genotoxic activity of paclitaxel by the in vitro micronucleus test in combination with fluorescent in situ hybridization of DNA centromeric probe and the alkaline single cell gel electrophoresis technique (comet assay) in human T-lymphocytes. Environ. Mol. Mutagen. 34:1999;269-278.
7. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res. 17:1988;184-191.
8. Ross G.M., McMillan T.J., Wilcox P., Collins A.R. The single cell microgel electrophoresis assay (comet assay) technical aspects and application, Report of the Fifth LH Gray Trust Workshop, Institute of Cancer Research. Mutat. Res. 337:1995;57-60.
9. Olive P.L. DNA damage and repair in individual cells: applications of the comet assay in radiobiology. Int. J. Radiat. Biol. 75:1999;395-405.
10. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.-C., Sosaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:2000;206-221.
11. Mohankumar M.N., Janani S., Karthikeya Prabhu B., Vivek Kumar P.R., Jeevanram R.K. DNA damage and integrity of UV-induced DNA repair in lymphocytes of smokers analyzed by the comet assay. Mutat. Res. 520:2002;179-187.
12. Rojas E., Valverde M., Lopez M.C., Naufal I., Sanchez I., Bizzarro P., Lopez I., Fortoul T.I., Ostrosky-Wegman P. Evaluation of DNA damage in exfoliated tear duct epithelial cells from individuals exposed to air pollution assessed by the single cell gel electrophoresis assay. Mutat. Res. 468:2000;11-17.
13. Leabilly P., Vigreux C., Lechevrel C., Ledemeney D., Godard T., Schel F., Le Talaër J.Y., Henry-Amar M., Gauduchon P. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: discussion of critical parameters and evaluation of seasonal variations in relation to pesticide exposure, Cancer Epidemiology. Biomarkers Prev. 7:1998;917-927.
14. Kopjar N., Garaj-Vrhovac V., Milas I. Assessment of chemotherapy-induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay. Teratogen. Carcinogen. Mutagen. 22:2002;13-30.
15. Olive P.L., Wlodek D., Durand R.E., Banath J.P. Factors influencing DNA migration from individual cells subject to gel electrophoresis. Exp. Cell. Res. 198:1992;259-267.
16. Nadin S.B., Vargas-Roig L.M., Ciocca D.R. A silver staining method for single-cell gel assay. J. Histochem. Cytochem. 49:2001;1183-1186.
17. Anderson D., Yu T.W., Philips B.J., Schemezer P. The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the comet assay. Mutat. Res. 307:1994;261-271.
18. Rajaee-Behbahani N., Schemezer P., Risch A., Rittgen W., Kayser K.W., Dienemann H., Schultz V., Drings P., Thiel S., Bartsch H. Altered DNA repair capacity and bleomicyn sensitivity as risk markers for non-small cell lung cancer. Int. J. Cancer. 95:2001;86-91.
19. Nadin S.B., Vargas-Roig L.M., Cuello-Carrion F.D., Ciocca D.R. Deoxyribonucleic acid damage induced by doxorubicin in peripheral blood mononuclear cells: possible roles for the stress response and the deoxyribonucleic acid repair process. Cell Stress Chaperones. 8:2003;361-372.
20. Horowitz S.B. Mechanism of action of taxol. Trends Pharmacol. Sci. 13:1992;134-136.
21. Tice R.R., Schneider E.L., Kram D., Thorne P. Cytokinetic analysis of the impaired proliferative response of peripheral lymphocytes from aged humans to phytohemagglutinin. J. Exp. Med. 00:1979;1029-1041.
22. Kaminskas E., Li J.C. Repair of DNA damage induced by oxygen radicals in human non-proliferating and proliferating lymphocytes. Mutat. Res. 274:1992;103-110.
23. Boerrigter M.E., Mullaart E., Vijg J. Induction and disappearance of DNA strand breaks in human peripheral blood lymphocytes and fibroblasts treated with methylmethanesulfonate. Exp. Cell. Res. 192:1991;61-66.
24. M. Sirover, Cell cycle regulation of DNA repair enzymes and pathways, Transformation of Human Diploid Fibroblasts, Boca Raton, CRC Press, 1990, pp. 29-54.
25. Spanswick V.J., Craddock C., Sekhar M., Mahendra P., Shankaranarayana P., Hughes R.G., Hochhauser D., Hartley J.A. Repair of DNA interstrand crosslinks as a mechanism of clinical resistance to melphalan in multiple myeloma. Blood. 100:2002;224-229.
26. Fedier A., Schwarz V.A., Walt H., Carpini R.D., Haller U., Fink D. Resistance to topoisomerase poisons due to loss of DNA mismatch repair. Int. J. Cancer. 93:2001;571-576.