The conserved L5 loop establishes the pre-powerstroke conformation of the kinesin-5 motor, Eg5

Biophysical Journal

Volumn 98, Issue 11, 2010, Pages 2619-2627

  Larson, Adam G ^a   Naber, Nariman ^b   Cooke, Roger ^b   Pate, Edward ^c   Rice, Sarah E ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RUMEX;

EID: 77952977790     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2010.03.014     Document Type: Article

References (35)

1. Mayer, T. U., T. M. Kapoor, ..., T. J. Mitchison. 1999. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 286:971-974.
2. Kapoor, T. M., T. U. Mayer, ..., T. J. Mitchison. 2000. Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J. Cell Biol. 150:975-988.
3. Skouflas, D. A., S. DeBonis, ..., F. Kozielski. 2006. S-trityl-L-cysteine is a reversible, tight binding inhibitor of the human kinesin Eg5 that specifically blocks mitotic progression. J. Biol. Chem. 281: 17559-17569.
4. Lad, L., L. Luo, ..., R. Sakowicz. 2008. Mechanism of inhibition of human KSP by ispinesib. Biochemistry. 47:3576-3585.
5. Brier, S., D. Lemaire, ..., F. Kozielski. 2004. Identification of the protein binding region, of S-trityl-L-cysteine, a new potent inhibitor of the mitotic kinesin Eg5. Biochemistry. 43:13072-13082.
6. Dagenbach, E. M., and S. A. Endow. 2004. A new kinesin tree. J. Cell Sci. 117:3-7.
7. Goodson, H. V., H. M. Warrick, and J. A. Spudich. 1999. Specialized conservation of surface loops of myosin: evidence that loops are involved in determining functional characteristics. J. Mol. Biol. 287:173-185.
8. Sweeney, H. L., S. S. Rosenfeld, ..., J. R. Sellers. 1998. Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocket. J. Biol. Chem. 273:6262-6270.
9. Siderovski, D. P., and F. S. Willard. 2005. The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits. Int. J. Biol. Sci. 1:51-66.
10. Maliga, Z., J. Xing, ..., S. S. Rosenfeld. 2006. A pathway of structural. changes produced by monastrol binding to Eg5. J. Biol. Chem. 281:7977-7982.
11. Cochran, J. C., T. C. Krzysiak, and S. P. Gilbert. 2006. Pathway of ATP hydrolysis by monomeric kinesin Eg5. Biochemistry. 45:12334-12344.
12. Cochran, J. C., C. A. Sontag, ..., S. P. Gilbert. 2004. Mechanistic analysis of the mitotic kinesin Eg5. J. Biol. Chem. 279:38861-38870.
13. Cochran, J. C., and S. P. Gilbert. 2005. ATPase mechanism of Eg5 in the absence of microtubules: insight into microtubule activation and allosteric inhibition by monastrol. Biochemistry. 44:16633-16648.
14. Rosenfeld, S. S., J. Xing, ..., P. H. King. 2005. Docking and rolling, a model of how the mitotic motor Eg5 works. J. Biol. Chem. 280:35684-35695.
15. Turner, J., R. Anderson, ..., R. Sakowicz. 2001, Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the necklinker. J. Biol. Chem. 276:25496-25502.
16. Larson, A. G., E. C. Landahl, and S. E. Rice. 2009. Mechanism of cooperative behaviour in systems of slow and fast molecular motors. Phys. Chem. Chem. Phys. 11:4890-4898.
17. Crowder, M. S., and R. Cooke. 1987. Orientation of spin-labeled nucleotides bound to myosin in glycerinated muscle fibers. Biophys. J. 51:323-333.
18. Wong, Y. L., K. A. Dietrich, ..., S. E. Rice. 2009. The kinesin-1 tail conformationally restricts the nucleotide pocket. Biophys. J. 96: 2799-2807.
19. Naber, N., T. J. Purcell, ..., R. Cooke. 2007. Dynamics of the nucleotide pocket of myosin measured by spin-labeled nucleotides. Biophys. J. 92:172-184.
20. Griffith, O. H., and P. C. Jost. 1976. Lipid Spin Labels in Biological Membranes. Academic Press, New York.
21. Alessi, D. R., J. E. Corrie, ..., D. R. Trentham. 1992. Synthesis and properties of a conformationally restricted spin-labeled analog of ATP and its interaction with, myosin and skeletal muscle. Biochemistry. 31:8043-8054.
22. Owenius, R., M. Osterlund, ..., U. Carlsson. 2001. Spin and fluorescent probing of the binding interface between tissue factor and factor Vila at multiple sites. Biophys. J. 81:2357-2369.
23. Sindelar, C. V., and K. H. Downing. 2007. The beginning of kinesin's force-generating cycle visualized at 9-A resolution. J. Cell Biol. 177:377-385.
24. Naber, N., T. J. Minehardt, ..., E. Pate. 2003. Closing of the nucleotide pocket of kinesin-family motors upon binding to microtubules. Science. 300:798-801.
25. Kaan, H. Y., V. Ulaganathan, ..., F. Kozielski. 2009. An allosteric transition trapped in an intermediate state of a new kinesin-inhibitor complex. Biochem. J. 425:55-60.
26. Krzysiak, T. C., T. Wendt, ..., A. Hoenger. 2006. A structural model for monastrol inhibition of dimeric kinesin Eg5. EMBO J. 25:2263-2273.
27. Sindelar, C. V., M. J. Budny, ..., R. Cooke. 2002. Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy. Nat. Struct. Biol. 9:844-848.
28. Maliga, Z., and T. J. Mitchison. 2006. Small-molecule and mutational analysis of allosteric Eg5 inhibition by monastrol. BMC Chem. Biol. 6:2.
29. Krzysiak, T. C., M. Grabe, and S. P. Gilbert. 2008. Getting in sync with dimeric Eg5. Initiation and regulation of the processive run. J. Biol. Chem. 283:2078-2087.
30. Rice, S., Y. Cui, ..., R. Cooke. 2003. Thermodynamic properties of the kinesin neck-region docking to the catalytic core. Biophys. J. 84; 1844-1854.
31. Yan, Y., V. Sardana, ..., L. C. Kuo. 2004. Inhibition of a mitotic motor protein: where, how, and conformational consequences. J. Mol. Biol. 335:547-554.
32. Parsegian, V. A., R. P. Rand, ..., D. C. Rau. 1986. Osmotic stress for the direct measurement of intermolecular forces. Methods Enzymol. 127:410-416.
33. Cochran, J. C., J. E. Gatial, 3rd, ..., S. P. Gilbert. 2005. Monastrol inhibition of the mitotic kinesin Eg5. J. Biol. Chem. 280:12658-12667.
34. Kim, K. S., S. Lu, ..., D. L. Roussell. 2006. Synthesis and SAR of pyrrolotriazine-4-one based Eg5 inhibitors. Bioorg. Med. Chem. Lett. 16:3937-3942.
35. Rice, S., A. W. Lin, ..., R. D. Vale. 1999. A structural change in the kinesin motor protein that drives motility. Nature. 402:778-784.