Closing of the nucleotide pocket of kinesin-family motors upon binding to microtubules

Science

Volumn 300, Issue 5620, 2003, Pages 798-801

  Naber, Nariman ^a   Minehardt, Todd J ^c,e   Rice, Sarah ^a   Chen, Xiaoru ^d   Grammer, Jean ^d   Matuska, Marija ^a   Vale, Ronald D ^a   Kollman, Peter A ^b   Car, Roberto ^c   Yount, Ralph G ^d   Cooke, Roger ^a,b   Pate, Edward ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c PRINCETON UNIVERSITY   (United States)

^d WASHINGTON STATE UNIVERSITY   (United States)

^e UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL BONDS; CONFORMATIONS; FREE ENERGY; HYDROLYSIS; NUCLEIC ACIDS; PARAMAGNETIC RESONANCE; PHOSPHATES; SPECTROSCOPIC ANALYSIS;

MICROTUBULES; NUCLEOTIDES;

ESTERS;

ADENOSINE DIPHOSPHATE; GUANINE NUCLEOTIDE BINDING PROTEIN; KINESIN; NUCLEOTIDE;

MICROBIOLOGY; PROTEIN;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ELECTRON SPIN RESONANCE; HYDROLYSIS; MICROTUBULE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE; REACTION ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS;

ADENINE NUCLEOTIDES; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; ELECTRON SPIN RESONANCE SPECTROSCOPY; HUMANS; HYDROGEN BONDING; HYDROLYSIS; KINESIN; MICROTUBULES; MODELS, MOLECULAR; MOLECULAR MOTOR PROTEINS; MOLECULAR PROBES; PROTEIN CONFORMATION; SPIN LABELS;

EID: 0038079445     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1082374     Document Type: Article

References (30)

1. F. J. Kull, R. D. Vale, R. J. Fletterick, J. Muscle Res. Cell Motil. 19, 877 (1998).
2. R. D. Vale, J. Cell Biol. 135, 291 (1996).
3. S. Sack, F. J. Kull, E. Mandelkow, Eur. J. Biochem. 262, 1 (1999).
4. W. R. Schief, J. Howard, Curr. Opin. Cell Biol. 13, 19 (2001).
5. E. P. Sablin, R. F. Fletterick, Curr. Opin. Struct. Biol. 11, 716 (2001).
6. F. J. Kull, S. A. Endow, J. Cell Sci. 115, 15 (2002).
7. S. R. Sprang, Curr. Opin. Struct. Biol. 7, 849 (1997).
8. W. L. Hubbell, H. S. McHaourab, C. Altenbach, M. A. Lietzow, Structure 4, 779 (1996).
9. D. D. Thomas, Annu. Rev. Physiol. 49, 691 (1987).
10. Materials and methods are available as supporting material on Science Online.
11. M. S. Crowder, R. Cooke, Biophys. J. 51, 323 (1987).
12. O. H. Griffith, P. C. Jost, in Spin Labeling Theory and Applications, L. J. Berliner, Ed. (Academic Press, NY, 1976), pp. 454-523.
13. F. J. Kull, E. P. Sablin, R. Lau, R.J. Fletterick, R. D. Vale, Nature 380, 550 (1996).
14. E. P. Sablin, F. J. Kull, R. Cooke, R. D. Vale, R. J. Fletterick, Nature 380, 555 (1996).
15. F. Kozielski et al., Cell 7, 1407 (1997).
16. S. Sack et al., Biochemistry 36, 16155 (1997).
17. A. M. Gulick, H. Song, S. A. Endow, I. Rayment, Biochemistry 37, 1769 (1998).
18. M. Kikkawa et al., Nature 411, 439 (2001).
19. Y. H. Song et al., EMBO J. 20, 6213 (2001).
20. J. Turner et al., J. Biol. Chem. 276, 25496 (2001).
21. M. Yun, X. Zhang, C. G. Park, H. W. Park, S. A. Endow, EMBO J. 20, 2611 (2001).
22. R. G. Yount, D. Lawson, I. Rayment, Biophys. J. 68, 44S (1995).
23. E. Pate, N. Naber, M. Matuska, K. Franks-Skiba, R. Cooke, Biochemistry 36, 12155 (1997).
24. D. E. Coleman et al., Science 265, 1405 (1994).
25. J. Goldberg, Cell 95, 237 (1998).
26. T. J. Minehardt, R. Cooke, E. Pate, P. A. Kollman, Biophys. J. 80, 1151 (2001).
27. N. Naber et al., Biophys. J. 84, 3190 (2003).
28. Coordinates have been deposited in the Protein Data Bank (10ZX). They may also be obtained from the authors.
29. Y. Z. Ma, E. W. Taylor, J. Biol. Chem. 272, 717 (1997).
30. This work was supported by NIH grants AR39643 (E.P.), AR42895 (R.C., R.D.V.), DK05915 (R.G.Y.), and GM29072 (P.A.K.). T.J.M. was a Princeton University Council on Science and Technology Postdoctoral Fellow. Molecular visualizations were done using the UCSF Computer Graphics Laboratory, supported by NIH grant RR1081 to T. Ferrin.