Spin and fluorescent probing of the binding interface between tissue factor and factor VIIa at multiple sites

Biophysical Journal

Volumn 81, Issue 4, 2001, Pages 2357-2369

  Owenius, Rikard ^a   Österlund, Maria ^a   Svensson, Magdalena ^a   Lindgren, Mikael ^a,c   Persson, Egon ^b   Freskgård, Per Ola ^b,d   Carlsson, Uno ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

^b NOVO NORDISK A S   (Denmark)

^c SWEDISH DEFENCE RESEARCH AGENCY   (Sweden)

^d Maxygen APS   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

4 CARBOXYGLUTAMIC ACID; BLOOD CLOTTING FACTOR 7A; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYE; GLOBULAR PROTEIN; PROTEINASE; THROMBOPLASTIN;

ACCELERATION; ARTICLE; BINDING SITE; COMPLEX FORMATION; ELECTRON SPIN RESONANCE; EXTRACELLULAR SPACE; FLUORESCENCE SPECTROSCOPY; MOLECULAR MODEL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; TIME;

EID: 0034811157     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75882-1     Document Type: Article

References (39)

1. The crystal structure of the complex of blood coagulation factor VIIa with soluble tissue factor
2. Nonlinear-least-squares analysis of slow-motion EPR spectra in one and two dimensions using a modified Levenberg-Marquardt algorithm
3. Folding of β-sheet proteins
4. Site-directed spin labeling of membrane proteins and peptide-membrane interactions
5. Theory of slow tumbling ESR spectra for nitroxides
6. 2+ and tissue factor studied using circular dichroism spectroscopy
7. Identification of the factor VIIa binding site on tissue factor by homologous loop swap and alanine scanning mutagenesis
8. Estimating slow-motional rotational times for nitroxides by electron spin resonance
9. High resolution probing of local conformational changes in proteins by the use of multiple labeling. Unfolding and self assembly of human carbonic anhydrase II monitored by spin, fluorescent, and chemical reactivity probes
10. Structural mapping of an aggregation nucleation site in a molten globule intermediate
11. Protein substrate binding induces conformational changes in the chaperonin GroEL. A suggested mechanism for unfoldase activity
12. Crystal structure of the extracellular region of human tissue factor
13. Identifying conformational changes with site-directed spin labeling
14. Recent advances in site-directed spin labeling of proteins
15. Watching proteins move using site-directed spin labeling
16. Analysis of the factor VIIa binding site on human tissue factor: Effects of tissue factor mutations on the kinetics and thermodynamics of binding
17. Detection of internal and overall dynamics of a two-atom-tethered spin-labeled DNA
18. Study of the influence of the microsurroundings on magneto-resonance parameters of spin-labeled human serum albumin in the 2 mm ESR range
19. Crystal structures of spin labeled T4 lysozyme mutants: Implications for the interpretation of EPR spectra in terms of structure
20. Probing local mobility in carbonic anhydrase: EPR of spin-labeled SH groups introduced by site-directed mutagenesis
21. The atomic structure of protein-protein recognition sites
22. Motion of spin-labeled side chains in T4 lysozyme. Correlation with protein structure and dynamics
23. The crystal structure of the extracellular domain of human tissue factor refined to 1.7 Å resolution
24. Structure of the extracellular domain of human tissue factor: Location of the factor VIIa binding site
25. Study of the effect of the medium on the magnetic-resonance parameters of nitroxyl radicals by high-resolution EPR spectroscopy
26. Spectroscopic probing of the influence of calcium and the G1a domain on the interaction between the first EGF domain in factor VIIa and tissue factor
27. Properties of spin and fluorescent labels at a receptor-ligand interface
28. EPR mapping of interactions between spin-labeled variants of human carbonic anhydrase II and GroEL: Evidence for increased flexibility of the hydrophobic core by the interaction
29. 2+ binding to the first epidermal growth factor-like domain of factor VIIa increases amidolytic activity and tissue factor affinity
30. Energetic contributions and topographical organization of ligand binding residues of tissue factor
31. Mutational mapping of functional residues in tissue factor: Identification of factor VII determinants in both structural modules of the predicted cytokine receptor homology domain
32. Calculating slow motional magnetic resonance spectra
33. Key ligand interface residues in tissue factor contribute independently to factor VIIa binding
34. Mapping the folding intermediate of human carbonic anhydrase II. Probing substructure by chemical reactivity and spin and fluorescence labeling of engineered cysteine residues
35. Amino acid sequence and post-translational modifications of human factor VIIa from plasma and transfected baby hamster kidney cells
36. Rotational dynamics of rigid, symmetric top macromolecules. Application to circular cylinders