S-trityl-L-cysteine is a reversible, tight binding inhibitor of the human kinesin Eg5 that specifically blocks mitotic progression

Journal of Biological Chemistry

Volumn 281, Issue 26, 2006, Pages 17559-17569

  Skoufias, Dimitrios A ^a   DeBonis, Salvatore ^a   Saoudi, Yasmina ^b   Lebeau, Luc ^c   Crevel, Isabelle ^d   Cross, Robert ^d   Wade, Richard H ^a   Hackney, David ^e   Kozielski, Frank ^a,f  

^a CEA GRENOBLE   (France)

^b INSERM   (France)

^c UNIVERSITÉ DE STRASBOURG   (France)

^d MARIE CURIE RESEARCH INSTITUTE   (United Kingdom)

^e CARNEGIE MELLON UNIVERSITY   (United States)

^f INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; CELLS; CYTOLOGY; ENZYMES; GROWTH KINETICS; STEREOCHEMISTRY; TUMORS;

CELL LINE GROWTH; HUMAN KINESIN; MITOTIC PROGRESSION; STEREOSPECIFICITY;

PROTEINS;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; CYSTEINE DERIVATIVE; KINESIN; MONASTROL; PROTEIN KINESIN EG5; S TRITYL LEVO CYSTEINE; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE PROGRESSION; CELL CYCLE S PHASE; CENTROSOME; CONTROLLED STUDY; DRUG BINDING; DRUG INHIBITION; DRUG MECHANISM; DRUG STRUCTURE; HUMAN; HUMAN CELL; IN VITRO STUDY; MITOSIS; PRIORITY JOURNAL; STEREOSPECIFICITY;

ADENOSINE DIPHOSPHATE; ANIMALS; ANTHRANILIC ACIDS; BONE NEOPLASMS; CATTLE; CELL DIVISION; CYSTEINE; DRUG DESIGN; HELA CELLS; HUMANS; KINESIN; MITOSIS; MITOTIC SPINDLE APPARATUS; OSTEOSARCOMA; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; STEREOISOMERISM;

EID: 33745867225     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M511735200     Document Type: Article

References (48)

1. Wood, K. W., Cornwell, W. D., and Jackson, J. R. (2001) Curr. Opin. Pharmacol. 1, 370-377
2. Miyamoto, D. T., Perlman, Z. E., Mitchison, T. J., and Shirasu-Hiza, M. (2003) Prog. Cell Cycle Res. 5, 349-360
3. Bergnes, G., Brejc, K., and Belmont, L. (2005) Curr. Top Med. Chem. 5, 127-145
4. Lawrence, C. J., Dawe, R. K., Christie, K. R., Cleveland, N., Dawson, S. C., Endow, S. A., Goldstein, L. S., Goodson, H. V., Hirokawa, N., Howard, J., Malmberg, R. L., McIntosh, J. R., Miki, H., Mitchison, T. J., Okada, Y., Reddy, A. S., Saxton, W. M., Schliwa, M., Scholey, J. M., Vale, R. D., Walczak, C. E., and Wordeman, L. (2004) J. Cell Biol. 167, 19-22
5. Blangy, A., Lane, H. A., d'Herin, P., Harper, M., Kress, M., and Nigg, E. A. (1995) Cell 83, 1159-1169
6. Weil, D., Garcon, L., Harper, M., Dumenil, D., Dautry, F., and Kress, M. (2002) Bio-Techniques 33, 1244-1248
7. Mayer, T. U., Kapoor, T. M., Haggarty, S. J., King, R. W., Schreiber, S. L., and Mitchison, T. J. (1999) Science 286, 971-974
8. Nakazawa, J., Yajima, J., Usui, T., Ueki, M., Takatsuki, A., Imoto, M., Toyoshima, Y. Y., and Osada, H. (2003) Chem. Biol. 10, 131-137
9. Hotha, S., Yarrow, J. C., Yang, J. G., Garrett, S., Renduchintala, K. V., Mayer, T. U., and Kapoor, T. M. (2003) Angew. Chem. Int. Ed. Engl. 42, 2379-2382
10. Sakowicz, R., Finer, J. T., Beraud, C., Crompton, A., Lewis, E., Fritsch, A., Lee, Y., Mak, J., Moody, R., Turincio, R., Chabala, J. C., Gonzales, P., Roth, S., Weitman, S., and Wood, K. W. (2004) Cancer Res. 64, 3276-3280
11. Cox, C. D., Breslin, M. J., Mariano, B. J., Coleman, P. J., Buser, C. A., Walsh, E. S., Hamilton, K., Huber, H. E., Kohl, N. E., Torrent, M., Yan, Y., Kuo, L. C., and Hartman, G. D. (2005) Bioorg. Med. Chem. Lett. 15, 2041-2045
12. DeBonis, S., Skoufias, D., Robin, G., Lebeau, L., Lopez, R., Margolis, R., Wade, R. H., and Kozielski, F. (2004) Mol. Cancer Ther. 3, 1079-1090
13. Canman, J. C., Cameron, L. A., Maddox, P. S., Straight, A., Tirnauer, J. S., Mitchison, T. J., Fang, G., Kapoor, T. M., and Salmon, E. D. (2003) Nature 424, 1074-1078
14. DeBonis, S., Simorre, J. P., Crevel, I., Lebeau, L., Skoufias, D. A., Blangy, A., Ebel, C., Gans, P., Cross, R., Hackney, D. D., Wade, R. H., and Kozielski, F. (2003) Biochemistry 42, 338-349
15. Maliga, Z., Kapoor, T. M., and Mitchison, T. J. (2002) Chem. Biol. 9, 989-996
16. Luo, L., Carson, J. D., Dhanak, D., Jackson, J. R., Huang, P. S., Lee, Y., Sakowicz, R., and Copeland, R. A. (2004) Biochemistry 43, 15258-15266
17. Cochran, J. C., Gatial, J. E., III, Kapoor, T. M., and Gilbert, S. P. (2004) J. Biol. Chem. 280, 12658-12667
18. Brier, S., Lemaire, D., DeBonis, S., Forest, E., and Kozielski, F. (2004) Biochemistry 43, 13072-13082
19. Paull, K. D., Lin, C. M., Malspeis, L., and Hamel, E. (1992) Cancer Res. 52, 3892-3900
20. Crevel, I., Lockhart, A., and Cross, R. A. (1997) J. Mol. Biol. 273, 160-170
21. Kozielski, F., Svergun, D. I., Zaccai, G., Wade, R. H., and Koch, M. H. J. (2001) J. Biol. Chem. 276, 1267-1275
22. Garcia-Saez, I., Yen, T., Wade, R. H., and Kozielski, F. (2004) J. Mol. Biol. 340, 1107-1116
23. Robley, C., Williams, J. R., and Lee, J. C. (1982) Methods Enzymol. 85, 376-385
24. Hackney, D. D., and Jiang, W. (2001) Methods Mol. Biol. 164, 65-71
25. Morrison, J. F. (1969) Biochim. Biophys. Acta 185, 269-286
26. Davis, F. M., Tsao, T. Y., Fowler, S. K., and Rao, P. N. (1983) Proc. Natl. Acad. Sci. U. S. A. 80, 2926-2930
27. Andreassen, P. R., Skoufias, D. A., and Margolis, R. L. (2004) Methods Mol. Biol. 281, 213-225
28. Copeland, R. A. (2000) Enzymes, 2nd Ed., pp. 305-317, John Wiley & Sons, Inc., New York
29. Vale, R. D., Reese, T. S., and Sheetz, M. P. (1985) (1985) Cell 42, 39-50
30. Brady, S. T. (1985) Nature 317, 73-75
31. Yen, T. J., Li, G., Schaar, B. T., Szilak, I., and Cleveland, D. W. (1992) Nature 359, 536-539
32. Nislow, C., Lombillo, V. A., Kuriyama, R., and McIntosh, J. R. (1992) Nature 359, 543-547
33. Lai, F., Fernald, A. A., Zhao, N., and Le Beau, M. M. (2000) Gene (Amst.) 248, 117-125
34. Mountain, V., Simerly, C., Howard, L., Ando, A., Schatten, G., and Compton, D. A. (1999) J. Cell Biol. 147, 351-366
35. Tokai, N., Fujimoto-Nishiyama, A., Toyoshima, Y., Yonemura, S., Tsukita, S., Inoue, J., and Yamamota, T. (1996) EMBO J. 15, 457-467
36. Kim, I. G., Jun, D. Y., Sohn, U., and Kim, Y. H. (1997) Biochim. Biophys. Acta 1359, 181-186
37. Sharp, D. J., Yu, K. R., Sisson, J. C., Sullivan, W., and Scholey, J. M. (1999) Nat. Cell Biol. 1, 51-54
38. Sharp, D. J., Brown, H. M., Kwon, M., Rogers, G. C., Holland, G., and Scholey, J. M. (2000) Mol. Biol. Cell 11, 241-253
39. Goshima, G., Wollman, R., Stuurman, N., Scholey, J. M., and Vale, R. D. (2005) Curr. Biol. 15, 1979-1988
40. Alley, M. C., Scudiero, D. A., Monks, A., Hursey, M. L., Czerwinski, M. J., Fine, D. L., Abbott, B. J., Mayo, J. G., Shoemaker, R. H., and Boyd, M. R. (1989) Cancer Res. 48, 589-601
41. Grever, M. R., Schepartz, S. A., and Chabner, B. A. (1992) Semin. Oncol. 19, 622-638
42. Boyd, M. R., and Paull, K. D. (1995) Drug Dev. Res. 34, 91-109
43. Geran, R. I., Greenberg, N. H., Macdonald, M. M., Schumacher, A. M., and Abbott, B. J. (1972) Cancer Chemother. Rep. 3, 1-87
44. Zhu, C., Zhao, J., Bibikova, M., Leverson, J. D., Bossy-Wetzel, E., Fan, J. B., Abraham, R. T., and Jiang, W. (2005) Mol. Biol. Cell 16, 3187-3199
45. Ganem, N. J., and Compton, D. A. (2004) J. Cell Biol. 166, 473-478
46. Marcus, A. I., Peters, U., Thomas, S. L., Garrett, S., Zelnak, A., Kapoor, T., and Giannakakou, P. (2005) J. Biol. Chem. 280, 11569-11577
47. Kelner, M. J., McMorris, T. C., Rojas, R. J., Trani, N. A., Velasco, T. R., Estes, L. A., and Suthipinijtham, P. (2002) Investig. New Drugs 20, 271-279
48. Xiao, D., Pinto, J. T., Gundersen, G. G., and Weinstein, I. B. (2005) Mol. Cancer Ther. 4, 1388-1398