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One of the most advanced models to date. This E. coli model covers 28% of the genes in the genome and contains regulatory information and has been checked for thermodynamic consistency.
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Feist A.M., Henry C.S., Reed J.L., Krummenacker M., Joyce A.R., Karp P.D., Broadbelt L.J., Hatzimanikatis V., and Palsson B.O. A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information. Mol Syst Biol 3 (2007) 121. One of the most advanced models to date. This E. coli model covers 28% of the genes in the genome and contains regulatory information and has been checked for thermodynamic consistency.
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BioGRID is an online resource for biological interactions. The database contains interaction data for many industrially relevant organisms, and contains physical as well as genetic interactions.
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Tandem genes offer a preferable approach to plasmids for multicopy expression of genes. CIChE allows high genetic stability, tunable expression, and does not require a selectable marker.
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