Fluorescence anisotropy assay for pharmacological characterization of ligand binding dynamics to melanocortin 4 receptors

Analytical Biochemistry

Volumn 402, Issue 1, 2010, Pages 32-39

  Veiksina, Santa ^a   Kopanchuk, Sergei ^a   Rinken, Ago ^a  

^a UNIVERSITY OF TARTU   (Estonia)

Author keywords

Cy3B NDP MSH; Fluorescence anisotropy; G protein coupled receptors; Ligand binding kinetics; Melanocortin 4 receptor; TAMRA NDP MSH

Indexed keywords

ANISOTROPY; DYNAMICS; FLUORESCENCE; PEPTIDES; RED SHIFT; VOLTAGE MEASUREMENT;

CONCENTRATION-DEPENDENT MANNERS; FLUORESCENCE ANISOTROPY; G PROTEIN COUPLED RECEPTORS; LIGAND BINDING; MELANOCORTIN 4 RECEPTOR; OPTIMAL EXPERIMENTAL CONDITIONS; PHARMACOLOGICAL ACTIVITY; PHARMACOLOGICAL CHARACTERIZATION;

LIGANDS;

ALPHA INTERMEDIN DERIVATIVE; BUFFER; MELANOCORTIN 4 RECEPTOR;

ANIMAL CELL; ANISOTROPY; ARTICLE; CELL MEMBRANE; CHEMICAL REACTION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG DESIGN; FLUORESCENCE ANALYSIS; FLUORESCENCE ANISOTROPY ASSAY; INSECT CELL; INTERMETHOD COMPARISON; LIGAND BINDING; MOLECULAR STABILITY; NONHUMAN; ONLINE MONITORING; PERFORMANCE; PRIORITY JOURNAL; RECEPTOR BINDING; REGULATORY MECHANISM; SIGNAL NOISE RATIO;

HEXAPODA;

EID: 77952744237     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.03.022     Document Type: Article

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